Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

The present study is aimed at the isolation and characterization of betulin from Entada africana. A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

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ANTIBACTERIAL ACTIVITY OF XANTHONE FROM ETHYL ACETATE EXTRACT OF THE STEAM BARK OF Garcinia picrorrhiza Miq

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.13.2.4346.250-261 ◽

2017 ◽

Vol 13 (2) ◽

pp. 250 ◽

Cited By ~ 1

Author(s):

Muharni Muharni ◽

Elfita Elfita ◽

Emil Pertiwi

Keyword(s):

Antibacterial Activity ◽

Bacillus Subtilis ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Stem Bark ◽

Salmonella Typhi ◽

Separation And Purification ◽

Chromatography Method ◽

Staphyloccocus Aureus ◽

Isolated Compound

A compound was isolated from the ethyl acetate extract of stem bark Garcinia picrorrhiza. The extraction was conducted by maseration. Separation and purification were done by chromatography method. The structure of compound was established using UV, IR, and 1H NMR spectroscopy. The antibacteria activity of the isolated compound was tested by paper disk difusion method and minimum inhibitory concentration (MIC) value was determined by using well difusion method examined against bacteria Escherechia coli, Salmonella typhi, Bacillus subtilis and Staphyloccocus aureus. The isolated compound was a yellow solids (43.8 mg) with melting point 171 – 172 oC. Based on spectroscopy data compared with data from the literature, the isolated compound is a known compound of oxygenated xanthone group with structure 1,4,5-trihydroxy-2-(3methylbut-2-enyl)xanthone. The compound exhibited an antibacterial activity against Bacillus subtilis only with MIC of 62.5 µg/mL.

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ISOLATION AND CHARACTERIZATION OF TERPENOID COMPOUNDS FROM LEAVES OF Macaranga beccariana Merr.

JURNAL KIMIA MULAWARMAN ◽

10.30872/jkm.v16i1.670 ◽

2018 ◽

Vol 16 (1) ◽

pp. 22 ◽

Cited By ~ 1

Author(s):

Doni Prio Atmoko ◽

Eva Marliana ◽

Erwin Erwin

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Fraction ◽

Gradient Elution ◽

Isolation And Characterization ◽

Rf Values ◽

Ft Ir ◽

Elution Method ◽

Layer Chromatography ◽

Chloroform Methanol

Isolation and characterization of terpenoid compounds from ethyl acetate fraction of Macaranga beccariana Merr. have been done. The separation process at ethyl acetate fraction using gravity column chromatography with gradient elution method. The obtained isolates were tested for purity using thin layer chromatography with various eluents namely n-hexane, chloroform, methanol, acetone and ethyl acetate with Rf values 0; 0.75; 0.80; 0.87 and 0.97 respectively. The terpenoids which were successfully isolated were white crystals with a mass of 0.0119 grams. UV analysis produced 1 peak at l 207.03 nm. Analysis of compounds with FT-IR indicated the functional groups O-H, aliphatic C-H, aliphatic C=C, C-O and C=O. Based on the results of the UV and FT-IR spectrum, the isolate compounds were thought to contain terpenoids.

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Isolation and Characterisation of Hydrolysable Tannin from Ethyl Acetate Portion of the Aerial Part of Phyllanthus amarus Schum. & Thonn

Asian Journal of Applied Chemistry Research ◽

10.9734/ajacr/2018/v2i3-430079 ◽

2019 ◽

pp. 1-10

Author(s):

Halima A. Umar ◽

Hamidu Usman ◽

Mustapha B. Abubakar ◽

Baba F. Mohammed ◽

Mohammed Babakura ◽

...

Keyword(s):

Ethyl Acetate ◽

Aerial Part ◽

Methanol Extract ◽

Phyllanthus Amarus ◽

Hydrolysable Tannin ◽

Isolation And Characterization ◽

Hydrolysable Tannins ◽

Isolated Compound ◽

Layer Chromatography

The present work involves extraction of phytochemicals from aerial part of Phyllanthus amarus Schum. & Thonn with n-hexane and 85% methanol. The isolation and characterization of Phytoconstituents was done from the methanol extract through portioning with chloroform and ethyl acetate. Fractionation and isolation (using column and thin layer chromatography respectively) of ethyl acetate column pooled portion afforded a compound coded as E-3.3C. The structure of the isolated compound was established on spectroscopic evidences (IR, 1HNMR, MS), which revealed the compound as 1-de (oxygalloyl)-2ˈ,3ˈ,-di-methoxy-amariin a hydrolysable tannins.

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Isolation and Characterization of New Compound and its Antibacterial Activities from Stem-Bark Extract of Barringtonia Asiatica

Annals of Advanced Biomedical Sciences ◽

10.23880/aabsc-16000140 ◽

2019 ◽

Vol 2 (2) ◽

Author(s):

Isaac John Umaru

Keyword(s):

Spectral Analysis ◽

Ethyl Acetate ◽

1H Nmr ◽

Chemical Constituents ◽

Radical Scavenging ◽

Stem Bark ◽

Chromatographic Technique ◽

Isolation And Characterization ◽

Column Chromatographic

Objective: This investigation involves the extraction, isolation, and characterization of chemical constituents from a Barringtonia family plant, Barringtonia asiatica followed by Antibacterial, cytotoxicity and evaluation of its antioxidant principles. Methods: The dried stem-bark powders were subjected to sequential soaking with polar and nonpolar solvents and extraction using rota-vap. Dichloromethane extract reveals the presence of significant amount of phytochemicals. The dichloromethane extract was subjected to isolation using column chromatographic analysis with solvents such as, dichloromethane, chloroform, hexane, ethyl acetate and methanol. Further, the isolated compound was subjected to thin layer chromatography technique and spectral analysis such as infrared, 1H-NMR, 13C-NMR, FT-IR, and mass spectroscopy. The antibacterial using agar disc method. Results: The compound was isolated in hexane: ethyl acetate (9:1) and dichloromethane: ethyl acetate in the solvent system in the ratio of 9:1 and 7:3, respectively using column chromatographic technique. On the basis of phytochemical, chromatographic, and spectral analysis, the isolated compounds were identified as Nerolidiol and Heneicosane. Conclusion: This compound was isolated for the first time from the stem-bark of Barringtonia asiatica. The in vitro antioxidant assay of isolated compounds has shown a dose-dependent increase in free radical scavenging activity using DPPH, the antibacterial and artemia salina cytotoxicity testing showed a significant result. The chromatographic separation led to the isolation of Nerolidiol (1) and Heneicosane (2). Their structures were determined by 1H-NMR, 13CNMR, IR and MS data analysis as well as by comparison of their data with the published values.

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Isolation, Characterization and Antibacterial Activity of ECDY Steroid from Roots of Polycarpaea corymbosa LAM

International Journal of Pharmaceutical Sciences Review and Research ◽

10.47583/ijpsrr.2021.v68i02.014 ◽

2021 ◽

Vol 68 (2) ◽

Author(s):

I. Carolin Nimila ◽

K.G. Lalitha ◽

R.Sambath kumar

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Petroleum Ether ◽

13C Nmr ◽

Bacterial Activity ◽

Pure Compound ◽

Simultaneous Extraction ◽

Soxhlet Apparatus ◽

Isolated Compound ◽

Benzene Extract

A new phytoecdysteroid (2- deoxy 20 hydroxy 22 cinnamoyl ecdysone 3, 25 diacetate) was isolated from the roots of Polycarpaea corymbosa Lam (family: caryophyllaceae).The powdered roots were extracted with petroleum ether ( 60-80? C), benzene, ethyl acetate and ethyl alcohol in soxhlet apparatus by simultaneous extraction. The highest yield of benzene extract was chosen for the isolation of ecdysteroid. The white solid pure compound was isolated and tested by TLC and respond positively for Liebermann - burchard reaction. Their structure has been established by UV, IR, 1H, 13C-NMR DEPT -90, DEPT -135 and EI- MS experiments. All the extracts and isolated compound were screened for anti-bacterial activity against B.subtilis, P.vulgaris, E.coli, P.aeruginosa by Kirbybauer method.

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Antibacterial Activity of Scopoletin from Stem Bark of Aleurites moluccana Against Salmonella typhi

Journal Of Tropical Pharmacy And Chemistry ◽

10.25026/jtpc.v5i1.218 ◽

2020 ◽

Vol 5 (1) ◽

pp. 29-32 ◽

Cited By ~ 1

Author(s):

Wisnu Cahyo Prabowo ◽

Risna Agustina

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Fraction ◽

Stem Bark ◽

Salmonella Typhi ◽

East Kalimantan ◽

Antibacterial Screening ◽

Microdilution Method ◽

The People ◽

Aleurites Moluccana

Background: Based on a previous survey of ethnopharmaceutical studies (local culture), it was known that the use of decoction of candlenut stem bark (Aleurites Moluccana) was used as typhoid medicine in the treatment tradition of the people of East Kalimantan. In antibacterial screening by diffusion test, methanol extract of stem bark A.moluccana showed excellent growth inhibition of Salmonella thypimurium. Working method: Simplicia of A.moluccana stem bark was extracted by maceration using methanol. Maserate was extracted liquid-liquid using n-hexane and ethyl acetate solvents. The hexane and ethyl acetate fraction was monitored for active spots with TLC bioautography. The active compound is separated using vacuum liquid chromatography and radial chromatography. Results: The fraction J of KCV and J2 subfraction results from radial chromatography provides antibacterial activity of S. thpimurium. From the J2 subfraction obtained pure isolates in the form of yellowish needle crystals. The isolate was tested for antibacterial S.thypimurium using the microdilution method with a value of MIC is 250 µg/ml. Based on spectroscopic data and comparing the published spectra of the compound, the elucidation of the isolate is Scopoletin (7-hydroxy-6-methoxycoumarin).

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TO STUDY THE ANTIBACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF CLAUSENA DENTATA (WILLD.) ROEM.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i1.35553 ◽

2019 ◽

pp. 69-73

Author(s):

ANNAMALAI MADURAM ◽

RAJU KAMARAJ

Keyword(s):

Antibacterial Activity ◽

Tamil Nadu ◽

Pathogenic Bacteria ◽

Volatile Oil ◽

Stem Bark ◽

Salmonella Typhi ◽

Chloroform Extract ◽

Klebsiella Pneumonia ◽

Human Pathogens ◽

Human Pathogenic Bacteria

Objectives: The objectives of the study were to study the antibacterial activity for the various extracts of Clausena dentata against human pathogens. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of C. dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study of microbiological properties is very scarce. Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India, and authenticated by Centre for Advanced Study in Botany, University of Madras, Chennai. The dry powder of stem bark was extracted with hexane, chloroform, and methanol. The extracts were subjected to qualitative phytochemical screening and antibacterial activity against human pathogenic bacteria such as Escherichia coli, Salmonella Typhi, Klebsiella pneumonia, Vibrio cholerae, and Staphylococcus aureus and compared with ciprofloxacin. Results: Qualitative chemical tests revealed the presence of various phytochemicals such as alkaloids, glycosides, carbohydrate, proteins and amino acids, phytosterols, and volatile oil. The antibacterial activity result reveals that all the extracts were are more active against V. cholerae. The activity against Pseudomonas aeruginosa was mild. Conclusion: The activity against V. cholerae was comparable with that of 5 μg/mL ciprofloxacin at the concentration of C. dentata 40 μg/mL. The orders of antibacterial activity against human pathogenic bacteria are hexane, methanol, and chloroform extract of C. dentata.

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Isolation and Characterization of Phages Active against Paenibacillus larvae Causing American Foulbrood in Honeybees in Poland

Viruses ◽

10.3390/v13071217 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1217

Author(s):

Ewa Jończyk-Matysiak ◽

Barbara Owczarek ◽

Ewa Popiela ◽

Kinga Świtała-Jeleń ◽

Paweł Migdał ◽

...

Keyword(s):

Biological Properties ◽

Field Application ◽

Paenibacillus Larvae ◽

American Foulbrood ◽

Isolation And Characterization ◽

Safety Studies ◽

Spreading Disease ◽

Intended Use ◽

Antibacterial Potential

The aim of this study was the isolation and characterization, including the phage effect on honeybees in laboratory conditions, of phages active against Paenibacillus larvae, the causative agent of American Foulbrood—a highly infective and easily spreading disease occurring in honeybee larva, and subsequently the development of a preparation to prevent and treat this dangerous disease. From the tested material (over 2500 samples) 35 Paenibacillus spp. strains were obtained and used to search for phages. Five phages specific to Paenibacillus were isolated and characterized (ultrastructure, morphology, biological properties, storage stability, and genome sequence). The characteristics were performed to obtain knowledge of their lytic potential and compose the final phage cocktail with high antibacterial potential and intended use of future field application. Preliminary safety studies have also been carried out on healthy bees, which suggest that the phage preparation administered is harmless.

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