Isolation and Characterization of Phytochemical Constituents and its Antibacterial Activity of Brassica oleracea var acephala

2019 ◽  
Vol 12 (1) ◽  
pp. 297
Author(s):  
U. S. Mahadeva Rao ◽  
C. Shanmuga Sundaram ◽  
J. Sivakumar
Keyword(s):  
Antibacterial Activity ◽  
Brassica Oleracea ◽  
Isolation And Characterization ◽  
Phytochemical Constituents

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

scholarly journals ISOLASI DAN KARAKTERISASI ENZIM b-1,3-ENDOGLUKANASE DARI TANAMAN KUBIS (Brassica oleracea cv. Capitata L.)

2010 ◽  
Vol 15 (2) ◽  
pp. 99-105
Author(s):  
Y. Sri Manuhara
Keyword(s):  
Ion Exchange ◽  
Brassica Oleracea ◽  
Hydrophobic Interaction Chromatography ◽  
Inhibition Zone ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Isolation And Characterization ◽  
Sds Page ◽  
Optimum Ph

Isolation and characterization of β-1,3-endoglucanase from cabbage (Brassica oleracea var. capitata L.) have been done. It showed 40° C of optimum temperature, and optimum pH is 7. After the purification with hydrophobic interaction chromatography and ion exchange chromatography, it’s activity was increased. Based on SDS-PAGE analysis, β-1,3-endoglucanase have molecular weight around 48 kD. Antifungal activity of β-1,3-endoglukanase show that it has best inhibition zone on Fusarium solanii at extract from ion exchange chromatography.

Isolation and characterization of a J domain protein that interacts with ARC1 from ornamental kale (Brassica oleracea var. acephala)

2015 ◽  
Vol 34 (5) ◽  
pp. 817-829 ◽  
Author(s):  
Xingguo Lan ◽  
Jia Yang ◽  
Mingming Cao ◽  
Yanhong Wang ◽  
Saneyuki Kawabata ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Isolation And Characterization ◽  
Ornamental Kale ◽  
J Domain ◽  
Domain Protein

scholarly journals Isolation and Characterization of Phenolic Compounds and Anthocyanins from Murta (Ugni molinae Turcz.) Fruits. Assessment of Antioxidant and Antibacterial Activity

Molecules ◽  
2015 ◽  
Vol 20 (4) ◽  
pp. 5698-5713 ◽  
Author(s):  
Maria Junqueira-Gonçalves ◽  
Lina Yáñez ◽  
Carolina Morales ◽  
Muriel Navarro ◽  
Rodrigo A. Contreras ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Isolation And Characterization ◽  
Ugni Molinae

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 3 (2) ◽  
pp. 28 ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

scholarly journals OPTIMASI SINTESIS DAN KARAKTERISASI SABUN CAIR DARI KEMIRI (Aleurites moluccana) DENGAN BANTUAN GELOMBANG ULTRASONIC

2021 ◽  
Vol 6 (1) ◽  
pp. 1-10
Author(s):  
Aman Santoso ◽  
◽  
Syafiuddin Syafiuddin ◽  
Dedek Sukarianingsih ◽  
Sumari Sumari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Fatty Acid Content ◽  
Chemical Properties ◽  
Ultrasonic Waves ◽  
Raw Material ◽  
Hazelnut Oil ◽  
Liquid Soap ◽  
Liquid Form ◽  
Isolation And Characterization

Liquid soap has become a basic necessity, especially during the Covid pandemic. The quality of soap is influenced by the type of raw material used. Candlenut oil saponification with potassium hydroxide produces liquid soap which has the potential to be anti-bacterial. Ultrasonic waves (US) produce cavitation which speeds up the reaction. This study aims: (1) Synthesis and characterization of liquid hazelnut oil soap, (2) to determine the antibacterial activity of liquid soap. The stages in this study were: (1) isolation and characterization of hazelnut oil, (2) making liquid soap at various alkaline concentrations with US (3) characterizing liquid soap (4) testing for antibacterial activity. The results showed that liquid soap can be made by saponification with KOH hazelnut oil, obtained physical properties of viscous liquid form, yellowish white color, distinctive aroma. The chemical properties of liquid soap have a pH of 8.46, a density of 1.10 g/mL, a free fatty acid content of 0.34%, a free alkaline content of 0.05%, a viscosity of 1,460 cSt and a surface tension of 4.33 x 10-4 N/m. Candlenut liquid soap has antibacterial activity in the weak category against E. coli bacteria and in the moderate category against S. aureus bacteria

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