Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling pathway

2020 ◽  
Vol 263 ◽  
pp. 113158 ◽  
Author(s):  
Shan Gao ◽  
Xiaoxue Xue ◽  
Jia Yin ◽  
Lina Gao ◽  
Zhu Li ◽  
...  
2019 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Issei Tomita ◽  
Shinji Kume ◽  
Kosuke Yamahara ◽  
Mako Yasuda-Yamahara ◽  
Naoko Takeda ◽  
...  

Author(s):  
Mei Zheng ◽  
Lizhuo Li ◽  
Yuqian Liu ◽  
Yun Liang ◽  
Xiaoyong Qi

This study was conducted to investigate the roles of ferritin in atherosclerosis. The mouse model of atherosclerosis was established by feeding ApoE knockout mice with a high-fat diet. The mice were then treated with ferritin-overexpressing and -silencing constructs, and assessed for interleukins (ILs) and matrix metalloproteinases (MMPs) levels using ELISA and Western blot analysis. After being fed with a high-fat diet, the ApoE knockout mice developed pro-atherogenic lipid profiles with elevated total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C). They also showed increased atherosclerotic lesions including narrowed lumen diameter, reduced lumen area, and increased plaque size. Following injection of the overexpression and silencing constructs, mRNA levels of ferritin were increased and decreased, respectively, and at the same time the atherosclerotic lesions were aggravated and alleviated, respectively. Further analysis indicated that silencing of ferritin gene reduced IL-1β and IL-10 levels while overexpressing ferritin increased them. On other hand, the TNF-α levels showed an opposite trend. MMP8, MMP12 and MMP13 levels were increased or decreased significantly after the mice were injected with ferritin over-expression or silencing vectors, respectively. Western blot analysis showed that compared to the control, overexpressing ferritin resulted in increased expression of p-JNK while silencing ferritin decreased the expression. Meanwhile, the levels of pc-Jun remained unchanged. Our work demonstrates that ferritin can regulate the progress of atherosclerosis via regulating the expression levels of MMPs and interleukins. Silencing ferritin inhibits the development of atherosclerosis and is, therefore, worth being further investigated as a potential therapeutic approach for this disease.


Heart ◽  
2012 ◽  
Vol 98 (Suppl 2) ◽  
pp. E117.2-E117
Author(s):  
Jiang Jin-jin ◽  
Wang Hui-feng ◽  
Jiang Jin-jin

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Qunfu Kang ◽  
Weihong Liu ◽  
Hongxu Liu ◽  
Mingxue Zhou

Compound Chuanxiong Capsule (CCC), a Chinese herbal compound, can exhibit antiatherosclerotic effect; however, its mechanism is still unclear. This study is designed to study the mechanism of CCC on atherosclerosis in the ApoE-knockout (ApoE−/−) mice fed with a high-fat diet. After 6 weeks of high-fat feeding, 40 ApoE−/−mice were randomized (n=10) and treated with lipitor, high-dose or low-dose CCC, or distilled water (ApoE−/−group) for 7 weeks. The blood lipids in serum and the plaque areas of the mice were measured and the mRNA expressions of phosphatidylinositol-3-kinases (PI3K), Akt, nuclear factor-kappa B (NF-κB), tumor necrosis factor-α(TNF-α), and interleukin-6 (IL-6) of the aortae were determined. The data showed that CCC can significantly decrease the levels of blood lipids, atherosclerosis index, and plaque areas and increase collagen proportion in plaques as compared with the untreated mice (p<0.05,p<0.01). In addition, CCC can significantly reduce the mRNA expressions of PI3K, Akt, NF-κB, IL-6, and TNF-αin the mice fed with a high-fat diet (p<0.001). Thus, we concluded that CCC can inhibit inflammatory reaction in the ApoE−/−mice fed with a high-fat diet. This mechanism may be attributed to regulating PI3K/Akt/NF-κB signaling pathway.


2021 ◽  
Vol 320 (1) ◽  
pp. H52-H65
Author(s):  
Shana R. Watson ◽  
Kara M. Cooper ◽  
Piaomu Liu ◽  
Nazli Gharraee ◽  
Liya Du ◽  
...  

The following major observations were made in this study: 1) aortic adventitial collagen fibers become more longitudinally oriented with aging in apolipoprotein E knockout mice fed a chow diet; 2) conversely, adventitial collagen fibers become more circumferentially oriented with aging in apoE knockout mice fed a high-fat diet; 3) adventitial collagen content increases significantly with age in mice on a high-fat diet; 4) these alterations in collagen organization occur largely in the absence of hemodynamic changes; and 5) circumferential reorientation of collagen is associated with decreased active force generation (contractility) in aged mice on a high-fat diet.


PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0155307 ◽  
Author(s):  
Carola I. F. Janssen ◽  
Diane Jansen ◽  
Martina P. C. Mutsaers ◽  
Pieter J. W. C. Dederen ◽  
Bram Geenen ◽  
...  

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