scholarly journals Genome Sequences of Antibiotic-Resistant Escherichia coli Isolated from Veal Calves in the United States

2021 ◽  
Author(s):  
Seon Woo Kim ◽  
Jo Ann S. Van Kessel ◽  
Bradd J. Haley
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
The United States ◽  
Genome Sequences ◽  
Antibiotic Resistant ◽  
Veal Calves

scholarly journals Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli, Including Sequence Type 131 (ST131), from Retail Chicken Breasts in the United States in 2013

2017 ◽  
Vol 83 (6) ◽  
Author(s):  
James R. Johnson ◽  
Stephen B. Porter ◽  
Brian Johnston ◽  
Paul Thuras ◽  
Sarah Clock ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Virulence Genes ◽  
Meat Products ◽  
The United States ◽  
Sequence Type ◽  
The Other ◽  
Antibiotic Resistant ◽  
Content Type ◽  
E Coli

ABSTRACT Chicken meat products are hypothesized to be vehicles for transmitting antimicrobial-resistant and extraintestinal pathogenic Escherichia coli (ExPEC) to consumers. To reassess this hypothesis in the current era of heightened concerns about antimicrobial use in food animals, we analyzed 175 chicken-source E. coli isolates from a 2013 Consumer Reports national survey. Isolates were screened by PCR for ExPEC-defining virulence genes. The 25 ExPEC isolates (12% of 175) and a 2:1 randomly selected set of 50 non-ExPEC isolates were assessed for their phylogenetic/clonal backgrounds and virulence genotypes for comparison with their resistance profiles and the claims on the retail packaging label (“organic,” “no antibiotics,” and “natural”). Compared with the findings for non-ExPEC isolates, the group of ExPEC isolates had a higher prevalence of phylogroup B2 isolates (44% versus 4%; P < 0.001) and a lower prevalence of phylogroup A isolates (4% versus 30%; P = 0.001), a higher prevalence of multiple individual virulence genes, higher virulence scores (median, 11 [range, 4 to 16] versus 8 [range, 1 to 14]; P = 0.001), and higher resistance scores (median, 4 [range, 0 to 8] versus 3 [range, 0 to 10]; P < 0.001). All five isolates of sequence type 131 (ST131) were ExPEC (P = 0.003), were as extensively resistant as the other isolates tested, and had higher virulence scores than the other isolates tested (median, 12 [range, 11 to 13] versus 8 [range, 1 to 16]; P = 0.005). Organic labeling predicted lower resistance scores (median, 2 [range, 0 to 3] versus 4 [range, 0 to 10]; P = 0.008) but no difference in ExPEC status or virulence scores. These findings document a persisting reservoir of extensively antimicrobial-resistant ExPEC isolates, including isolates from ST131, in retail chicken products in the United States, suggesting a potential public health threat. IMPORTANCE We found that among Escherichia coli isolates from retail chicken meat products purchased across the United States in 2013 (many of these isolates being extensively antibiotic resistant), a minority had genetic profiles suggesting an ability to cause extraintestinal infections in humans, such as urinary tract infection, implying a risk of foodborne disease. Although isolates from products labeled “organic” were less extensively antibiotic resistant than other isolates, they did not appear to be less virulent. These findings suggest that retail chicken products in the United States, even if they are labeled “organic,” pose a potential health threat to consumers because they are contaminated with extensively antibiotic-resistant and, presumably, virulent E. coli isolates.

scholarly journals High-Quality Whole-Genome Sequences for 21 Enterotoxigenic Escherichia coli Strains Generated with PacBio Sequencing

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Peyton Smith ◽  
Rebecca L. Lindsey ◽  
Lori A. Rowe ◽  
Dhwani Batra ◽  
Devon Stripling ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
The United States ◽  
Enterotoxigenic Escherichia Coli ◽  
Whole Genome ◽  
Cruise Ship ◽  
Genome Sequences ◽  
Pacbio Sequencing ◽  
High Quality ◽  
Surveillance Studies

ABSTRACT Enterotoxigenic Escherichia coli (ETEC) is an important diarrheagenic pathogen. We report here the high-quality whole-genome sequences of 21 ETEC strains isolated from patients in the United States, international diarrheal surveillance studies, and cruise ship outbreaks.

scholarly journals Detection of Multidrug-Resistant Salmonella entericaSerotype typhimurium DT104 Based on a Gene Which Confers Cross-Resistance to Florfenicol and Chloramphenicol

1999 ◽  
Vol 37 (5) ◽  
pp. 1348-1351 ◽  
Author(s):  
Lance F. Bolton ◽  
Lynda C. Kelley ◽  
Margie D. Lee ◽  
Paula J. Fedorka-Cray ◽  
John J. Maurer
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Drug Resistance ◽  
The United States ◽  
Multidrug Resistant ◽  
Cross Resistance ◽  
Antibiotic Resistant ◽  
Chloramphenicol Resistance ◽  
Presumptive Identification ◽  
Drug Resistance Profile

Salmonella enterica serotype typhimurium(S. typhimurium) DT104 (DT104) first emerged as a major pathogen in Europe and is characterized by its pentadrug-resistant pattern. It has also been associated with outbreaks in the United States. The organism typically carries resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline. The mechanism of chloramphenicol resistance in DT104 was determined by producing antibiotic-resistant Escherichia coli host strain clones from DT104 DNA. DNA from chloramphenicol-resistant clones was sequenced, and probes specific for the genes floS. typhimurium (floSt ),int, invA, and spvC were produced for colony blot hybridizations. One hundred nine Salmonellaisolates, including 44 multidrug-resistant DT104 isolates, were tested to evaluate the specificities of the probes. The genefloSt , reported in this study, confers chloramphenicol and florfenicol resistance on S. typhimurium DT104. Florfenicol resistance is unique to S. typhimurium DT104 and multidrug-resistant S. typhimurium isolates with the same drug resistance profile among all isolates evaluated. Of 44 DT104 isolates tested, 98% were detected based on phenotypic florfenicol resistance and 100% had the floSt -positive genotype. Resistances to florfenicol and chloramphenicol are conferred by the genefloSt , described in this paper. Presumptive identification of S. typhimurium DT104 can be made rapidly based on the presence of the floSt gene or its resulting phenotype.

scholarly journals Genome Sequences of Eight Shiga Toxin-Producing Escherichia coli Strains Isolated from a Produce-Growing Region in California

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Craig T. Parker ◽  
Kerry K. Cooper ◽  
Steven Huynh ◽  
Timothy P. Smith ◽  
James L. Bono ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Shiga Toxin ◽  
Water Samples ◽  
The United States ◽  
Foodborne Illness ◽  
Genome Sequences ◽  
Content Type ◽  
Leafy Greens ◽  
Growing Region

Produce contaminated with Shiga toxin-producing Escherichia coli (STEC) is a continuing source of foodborne illness in the United States. This report documents the complete genome sequences of eight STEC strains isolated from livestock and water samples taken from a major agricultural region for leafy greens in California.

scholarly journals PacBio Genome Sequences of Eight Escherichia albertii Strains Isolated from Humans in the United States

2019 ◽  
Vol 8 (9) ◽  
Author(s):  
Rebecca L. Lindsey ◽  
Lori A. Rowe ◽  
Dhwani Batra ◽  
Peyton Smith ◽  
Nancy A. Strockbine
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Virulence Genes ◽  
The United States ◽  
Genome Sequences ◽  
Emerging Pathogen ◽  
Content Type ◽  
E Coli

Escherichia albertii is an emerging pathogen that is closely related to Escherichia coli and can carry some of the same virulence genes as E. coli. Here, we report the release of Illumina-corrected PacBio sequences for eight E. albertii genomes.

Factors affecting the geographic variability of antibiotic-resistant healthcare-associated infections in the United States using the CDC Antibiotic Resistance Patient Safety Atlas

2019 ◽  
Vol 40 (05) ◽  
pp. 597-599 ◽  
Author(s):  
Julianne N. Kubes ◽  
Scott K. Fridkin
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Healthcare Facility ◽  
The United States ◽  
Antibiotic Prescribing ◽  
Healthcare Associated Infections ◽  
Geographic Variability ◽  
Antibiotic Resistant ◽  
Factors Affecting ◽  
Healthcare Associated

AbstractWe utilized publicly available data from the Centers for Disease Control to explore possible causes of state-to-state variability in antibiotic-resistant healthcare-associated infections. Outpatient antibiotic prescribing rates of fluoroquinolones and cephalosporins explained some variability in extended-spectrum cephalosporin-resistant Escherichia coli after adjusting for differences in age and healthcare facility composition.

scholarly journals Draft Genome Sequences of Three European Laboratory Derivatives from Enterohemorrhagic Escherichia coli O157:H7 Strain EDL933, Including Two Plasmids: TABLE 1 

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Lea Fellner ◽  
Christopher Huptas ◽  
Svenja Simon ◽  
Anna Mühlig ◽  
Siegfried Scherer ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Draft Genome ◽  
The United States ◽  
Enterohemorrhagic Escherichia Coli ◽  
Original Strain ◽  
Escherichia Coli O157 ◽  
Genome Sequences ◽  
E Coli ◽  
European Laboratory

Escherichia coli O157:H7 EDL933, isolated in 1982 in the United States, was the first enterohemorrhagic E. coli (EHEC) strain sequenced. Unfortunately, European labs can no longer receive the original strain. We checked three European EDL933 derivatives and found major genetic deviations (deletions, inversions) in two strains. All EDL933 strains contain the cryptic EHEC-plasmid, not reported before.

scholarly journals Complete Genome Sequences of Two Shiga Toxin-Producing Escherichia coli Strains from Serotypes O119:H4 and O165:H25

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Rebecca L. Lindsey ◽  
Kristen Knipe ◽  
Lori Rowe ◽  
Lisley Garcia-Toledo ◽  
Vladimir Loparev ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Shiga Toxin ◽  
Complete Genome ◽  
Foodborne Pathogen ◽  
The United States ◽  
Whole Genome ◽  
Genome Sequences ◽  
Clinical Cases

Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen. Here, we report complete whole-genome sequences for two STEC strains of serotypes O119:H4 and O165:H25 isolated from clinical cases in the United States.

scholarly journals Sequences of Seven Complete Genomes of Human Parvovirus B19

2018 ◽  
Vol 7 (11) ◽  
Author(s):  
Yuhuan Qiu ◽  
Zehui Zhao ◽  
Jianming Qiu
Keyword(s):  
United States ◽  
Human Plasma ◽  
Parvovirus B19 ◽  
The United States ◽  
Human Parvovirus B19 ◽  
Genome Sequences ◽  
Complete Genomes ◽  
Inverted Terminal Repeats ◽  
Terminal Repeats

We are reporting the sequences of seven complete genomes of parvovirus B19, which were extracted from human plasma specimens collected in the United States. The seven B19 genome sequences, which are 5,596 nucleotides long and include the inverted terminal repeats (ITRs), share an identity of 96.73%.

scholarly journals Corrected Genome Sequence of Acinetobacter baumannii Strain AB0057, an Antibiotic-Resistant Isolate from Lineage 1 of Global Clone 1

2017 ◽  
Vol 5 (35) ◽  
Author(s):  
Mohammad Hamidian ◽  
Pratap Venepally ◽  
Ruth M. Hall ◽  
Mark D. Adams
Keyword(s):  
United States ◽  
Acinetobacter Baumannii ◽  
Genome Sequence ◽  
The United States ◽  
Targeted Sequencing ◽  
Resistant Isolate ◽  
Illumina Hiseq ◽  
Antibiotic Resistant ◽  
Content Type ◽  
Pcr Products

ABSTRACT Extensively antibiotic-resistant Acinetobacter baumannii isolate AB0057 recovered in the United States in 2004 was one of the first global clone 1 isolates to be completely sequenced. Here, the complete 4.05-Mb genome sequence (chromosome and one plasmid) has been revised using Illumina HiSeq data and targeted sequencing of PCR products.

Sign in / Sign up

Export Citation Format

Share Document