Cow dung is an ideal fermentation medium for amylase production in solid-state fermentation by Bacillus cereus

Amylase is an amylolytic enzyme used in food industry which is generally produced by Aspergillus spp. under solid state fermentation. The present study is concerned with the isolation, screening and selection of suitable strains of Aspergillus spp. and optimization of cultural conditions for the biosynthesis of amylase. Rice and wheat brans were used as substrates which are readily available inexpensive raw materials for amylase production. From 85 samples of rice and wheat grains, 55 colonies obtained on potato dextrose agar (PDA) were suspected to be Aspergillus oryzae and only 35 colonies possessed the morphological characteristics similar to that of A. oryzae indicating the isolates were most likely the strains of A. oryzae. Of all the fungal isolates of Aspergillus spps., Asp.31 gave maximum production of amylase (720.782 IUgds-1) in solid state fermentation media. This strain was selected as a parental strain for optimization for cultural conditions. The obtained data were analyzed using SPSS- 11.5 program. Of all the substrates (rice bran, wheat bran and their mixture), rice bran was the best for producing amylase of highest activity 611.614 IUgds-1.The highest enzyme activity of 698.749 IUgds-1 was observed at 50% initial moisture level of the substrate. The optimum temperature was 25°C for producing the crude amylase enzyme with amylase activity of 577.757 IUgds-1. Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 67-74 DOI: http://dx.doi.org/10.3126/njst.v14i1.8924

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Alpha-amylase production byBacillus subtilis CM3 in solid state fermentation using cassava fibrous residue

Journal of Basic Microbiology ◽

10.1002/jobm.200710132 ◽

2007 ◽

Vol 47 (5) ◽

pp. 417-425 ◽

Cited By ~ 17

Author(s):

M.R. Swain ◽

R.C. Ray

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Alpha Amylase ◽

Amylase Production ◽

Fibrous Residue

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Cultural conditions optimization for production of β-galactosidase from Bacillus licheniformis ATCC 12759 under solid-state fermentation

Turkish Journal of Biochemistry ◽

10.1515/tjb-2017-0153 ◽

2018 ◽

Vol 43 (3) ◽

pp. 240-247 ◽

Cited By ~ 1

Author(s):

Nurullah Akcan

Keyword(s):

Solid State ◽

Moisture Content ◽

Bacillus Licheniformis ◽

Solid State Fermentation ◽

Incubation Temperature ◽

Fermentation Medium ◽

Inoculum Level ◽

Cultural Conditions ◽

Initial Ph ◽

Fermentation Parameters

AbstractObjective:The aim of this work was to study the optimal cultivation conditions for β-galactosidase production byBacillus licheniformisATCC 12759.Materials and methods:The screening of β-galactosidase production fromB. licheniformisATCC 12759 was performed by solid state fermentation method on media rich with rice bran (RB). Different factors were tested for the optimization of β-galactosidase production.Results:Certain fermentation parameters involving incubation time, incubation temperature, inoculum level, moisture content, initial pH, agitation speed, size of fermentation medium and optimum temperature of β-galactosidase activity were studied separately. Maximal amount of β-galactosidase production was obtained when solid-state fermentation (SSF) was carried out using RB, having inoculum level 35%, moisture content of 20%, initial pH 7.5 at 37°C for 48 h.Conclusion:Results indicated that optimal fermentation conditions play a key role in the maximum production of β-galactosidase fromB. licheniformisATCC 12759. This study shows the potential of the studied enzymes to be promoting candidates for the degradation of lactose and production of important bioproducts.

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Solid-state fermentation with orange waste: optimization of Laccase production from Pleurotus pulmonarius CCB-20 and decolorization of synthetic dyes

Acta Scientiarum Biological Sciences ◽

10.4025/actascibiolsci.v42i1.52699 ◽

2020 ◽

Vol 42 ◽

pp. e52699 ◽

Cited By ~ 3

Author(s):

Alex Graça Contato ◽

Fabíola Dorneles In´ácio ◽

Tatiane Brugnari ◽

Caroline Aparecida Vaz de Araújo ◽

Giselle Maria Maciel ◽

...

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Laccase Activity ◽

Fermentation Medium ◽

Laccase Production ◽

Synthetic Dyes ◽

Pleurotus Pulmonarius ◽

Biotechnological Potential ◽

Orange Waste ◽

Oxidoreductase Enzymes

Laccases are oxidoreductase enzymes that have the ability to oxidize phenolic substrates. Its biotechnological potential has been greatly explored in many areas as biotechnology industry, bioremediation of dyes, food industry and environmental microbiology. The aim of this study was maximize the laccase production by Pleurotus pulmonarius (Fr.) Quélet in solid-state fermentation (SSF) using orange waste as substrate. After optimization the capability of the crude laccase to decolorize dyes was analyzed. The fermentation medium in the solid-state was optimized by applying a factorial design. After statistics optimization, laccase activity increased two times. The laccase activity appears to be correlated with the ability of crude extract to decolorize some industrial dyes. The optimized laccase was characterized with respect to optimum pH, influence of temperature and salts. Our results demonstrate that P. pulmonarius was an efficient producer of an important industrial enzyme, laccase, in a cheap solid-state system using orange waste as substrate.

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