Mapping and functional analysis of high-copper accumulation mutant oshc1 in rice

ABSTRACTCopper is an essential transition metal for all eukaryotes. In mammals, intestinal copper absorption is mediated by the ATP7A copper transporter, whereas copper excretion occurs predominantly through the biliary route and is mediated by the paralog ATP7B. Both transporters have been shown to be recycled actively between the endosomal network and the plasma membrane by a molecular machinery known as the COMMD/CCDC22/CCDC93 or CCC complex. In fact, mutations in COMMD1 can lead to impaired biliary copper excretion and liver pathology in dogs and in mice with liver-specific Commd1 deficiency, recapitulating aspects of this phenotype. Nonetheless, the role of the CCC complex in intestinal copper absorption in vivo has not been studied, and the potential redundancy of various COMMD family members has not been tested. In this study, we examined copper homeostasis in enterocyte-specific and hepatocyte-specific COMMD gene-deficient mice. We found that, in contrast to effects in cell lines in culture, COMMD protein deficiency induced minimal changes in ATP7A in enterocytes and did not lead to altered copper levels under low- or high-copper diets, suggesting that regulation of ATP7A in enterocytes is not of physiological consequence. By contrast, deficiency of any of three COMMD genes (Commd1, Commd6 or Commd9) resulted in hepatic copper accumulation under high-copper diets. We found that each of these deficiencies caused destabilization of the entire CCC complex and suggest that this might explain their shared phenotype. Overall, we conclude that the CCC complex plays an important role in ATP7B endosomal recycling and function.

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Excessive Hepatic Copper Accumulation in Jaundiced Rats Fed a High-Copper Diet

Biological Trace Element Research ◽

10.1385/bter:88:3:255 ◽

2002 ◽

Vol 88 (3) ◽

pp. 255-270

Author(s):

A. C. Beynen ◽

S. Yu ◽

R. Van Der Meer

Keyword(s):

Copper Accumulation ◽

Hepatic Copper ◽

High Copper

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Differential behavior of the summer cover crops in the absorption and translocation of copper

Ciência Rural ◽

10.1590/0103-8478cr20180005 ◽

2018 ◽

Vol 48 (12) ◽

Cited By ~ 3

Author(s):

Diogo Vendruscolo ◽

Natielo Almeida Santana ◽

Kelen Müller Souto ◽

Paulo Ademar Avelar Ferreira ◽

George Wellington Bastos de Melo ◽

...

Keyword(s):

Cover Crops ◽

Contaminated Soils ◽

Pennisetum Glaucum ◽

Dry Mass ◽

Paspalum Notatum ◽

Copper Accumulation ◽

Canavalia Ensiformis ◽

Plant Parts ◽

Phosphorus Absorption ◽

High Copper

ABSTRACT: Phytoremediation is an alternative technique used to treat copper-contaminated soils. The objective of this research was to explore the behavior of nine summer cover crops regarding the growth, absorption and translocation of copper in soils with contamination levels exceeding the Value of Prevention, with a view to selecting plants for phytoremediation programs. In the experiments the Cambisol was contaminated with copper, added in doses of 0, 100, 200, 400, 500 and 600mg kg-1, in which the following nine plants were cultivated under greenhouse conditions: Canavalia ensiformis, Cajanus cajan, Dolichos lablad, Mucuna cinereum, Mucuna aterrima, Crotalaria juncea, Crotalaria spectabilis, Pennisetum glaucum and Paspalum notatum. At 90 days after sowing the plants were evaluated for dry mass of shoot and root, Cu, N, P, K, Ca, Zn, and Fe levels in the shoot and Cu in the roots. High soil Cu levels induced a decline in the phosphorus absorption by the plants. Canavalia ensiformis displayed high potential for phytoextraction as these plants could translocate high copper concentrations to the aerial plant parts, while the Mucuna cinereum and M. aterrima are indicated for the copper phytostabilization programs, due to the high copper accumulation in their root systems.

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Changes in copper, zinc and cadmium distributions in the liver of Formosan squirrels with characteristic high copper accumulation

Metallomics ◽

10.1039/c9mt00204a ◽

2019 ◽

Vol 11 (10) ◽

pp. 1753-1758

Author(s):

Yoshinari Suzuki ◽

Yasumitsu Ogra ◽

Noboru Machida ◽

Izumi Watanabe

Keyword(s):

Copper Toxicity ◽

Copper Accumulation ◽

High Copper ◽

Copper Zinc

We investigated the copper toxicity and the role of metallothionein in Formosan squirrels, which are newly discovered copper-accumulating animals.

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Clinical Issues: What’s in a Word? A Functional Analysis of Word Learning

Perspectives on Language Learning and Education ◽

10.1044/lle12.3.4 ◽

2005 ◽

Vol 12 (3) ◽

pp. 4-8 ◽

Cited By ~ 7

Author(s):

Prahlad Gupta

Keyword(s):

Functional Analysis ◽

Word Learning ◽

Clinical Issues

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Review for "High‐throughput single‐cell sequencing of paired TCRα and TCRβ genes for the direct expression‐cloning and functional analysis of murine T cell receptors"

10.1002/eji.201848030/v1/review1 ◽

2019 ◽

Keyword(s):

Functional Analysis ◽

T Cell ◽

Single Cell ◽

High Throughput ◽

T Cell Receptors ◽

Expression Cloning ◽

Cell Receptors ◽

Single Cell Sequencing

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Case Formulation and Design of Behavioral Treatment Programs

European Journal of Psychological Assessment ◽

10.1027//1015-5759.19.3.164 ◽

2003 ◽

Vol 19 (3) ◽

pp. 164-174 ◽

Cited By ~ 27

Author(s):

Stephen N. Haynes ◽

Andrew E. Williams

Keyword(s):

Functional Analysis ◽

Behavior Problems ◽

Behavioral Treatment ◽

Clinical Case ◽

Relative Magnitude ◽

Mechanisms Of Action ◽

Treatment Programs ◽

Case Formulation ◽

Functional Relations ◽

Causal Variables

Summary: We review the rationale for behavioral clinical case formulations and emphasize the role of the functional analysis in the design of individualized treatments. Standardized treatments may not be optimally effective for clients who have multiple behavior problems. These problems can affect each other in complex ways and each behavior problem can be influenced by multiple, interacting causal variables. The mechanisms of action of standardized treatments may not always address the most important causal variables for a client's behavior problems. The functional analysis integrates judgments about the client's behavior problems, important causal variables, and functional relations among variables. The functional analysis aids treatment decisions by helping the clinician estimate the relative magnitude of effect of each causal variable on the client's behavior problems, so that the most effective treatments can be selected. The parameters of, and issues associated with, a functional analysis and Functional Analytic Clinical Case Models (FACCM) are illustrated with a clinical case. The task of selecting the best treatment for a client is complicated because treatments differ in their level of specificity and have unequally weighted mechanisms of action. Further, a treatment's mechanism of action is often unknown.

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