Efficient mRNA electroporation of peripheral blood mononuclear cells to detect memory T cell responses for immunomonitoring purposes

2010 ◽  
Vol 354 (1-2) ◽  
pp. 1-10 ◽  
Author(s):  
K. Van Camp ◽  
N. Cools ◽  
B. Stein ◽  
A. Van de Velde ◽  
H. Goossens ◽  
...  
Keyword(s):  
T Cell ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
T Cell Responses ◽  
Peripheral Blood Mononuclear ◽  
Memory T Cell ◽  
Cell Responses ◽  
Blood Mononuclear Cells ◽  
Mrna Electroporation

scholarly journals Whole blood can be used as an alternative to isolated peripheral blood mononuclear cells to measure in vitro specific T-cell responses in human samples

2021 ◽  
pp. 112940
Author(s):  
Philippe Moris ◽  
Aurélie Bellanger ◽  
Opokua Ofori-Anyinam ◽  
Erik Jongert ◽  
Juan-Pablo Yarzabal Rodriguez ◽  
...  
Keyword(s):  
T Cell ◽  
Peripheral Blood Mononuclear Cells ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
T Cell Responses ◽  
Peripheral Blood Mononuclear ◽  
Cell Responses ◽  
Blood Mononuclear Cells

Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina)

1993 ◽  
Vol 37 (3-4) ◽  
pp. 217-230 ◽  
Author(s):  
Rik L. de Swart ◽  
Ruud M.G. Kluten ◽  
Carla J. Huizing ◽  
Lies J. Vedder ◽  
Peter J.H. Reijnders ◽  
...  
Keyword(s):  
T Cell ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
T Cell Responses ◽  
Phoca Vitulina ◽  
Harbour Seal ◽  
Peripheral Blood Mononuclear ◽  
Cell Responses ◽  
Blood Mononuclear Cells

scholarly journals Dengue‐Specific T Cell Responses in Peripheral Blood Mononuclear Cells Obtained prior to Secondary Dengue Virus Infections in Thai Schoolchildren

2002 ◽  
Vol 185 (12) ◽  
pp. 1697-1703 ◽  
Author(s):  
Marlou M. Mangada ◽  
Timothy P. Endy ◽  
Ananda Nisalak ◽  
Supamit Chunsuttiwat ◽  
David W. Vaughn ◽  
...  
Keyword(s):  
T Cell ◽  
Dengue Virus ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
T Cell Responses ◽  
Virus Infections ◽  
Peripheral Blood Mononuclear ◽  
Cell Responses ◽  
Blood Mononuclear Cells

Immunization With Melan-A Peptide-Pulsed Peripheral Blood Mononuclear Cells Plus Recombinant Human Interleukin-12 Induces Clinical Activity and T-Cell Responses in Advanced Melanoma

2003 ◽  
Vol 21 (12) ◽  
pp. 2342-2348 ◽  
Author(s):  
Amy C. Peterson ◽  
Helena Harlin ◽  
Thomas F. Gajewski
Keyword(s):  
T Cell ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
T Cell Responses ◽  
Advanced Melanoma ◽  
Interleukin 12 ◽  
Clinical Activity ◽  
Peripheral Blood Mononuclear ◽  
Cell Responses ◽  
Blood Mononuclear Cells

Purpose: Preclinical studies showed that immunization with peripheral blood mononuclear cells (PBMC) loaded with tumor antigen peptides plus interleukin-12 (IL-12) induced CD8+ T-cell responses and tumor rejection. We recently determined that recombinant human (rh) IL-12 at 30 to 100 ng/kg is effective as a vaccine adjuvant in patients. A phase II study of immunization with Melan-A peptide-pulsed PBMC + rhIL-12 was conducted in 20 patients with advanced melanoma. Patients and Methods: Patients were HLA-A2–positive and had documented Melan-A expression. Immunization was performed every 3 weeks with clinical re-evaluation every three cycles. Immune responses were measured by ELISpot assay before and after treatment and through the first three cycles, and were correlated with clinical outcome. Results: Most patients had received prior therapy and had visceral metastases. Nonetheless, two patients achieved a complete response, five patients achieved a minor or mixed response, and four patients had stable disease. The median survival was 12.25 months for all patients and was not yet reached for those with a normal lactate dehydrogenase. There were no grade 3 or 4 toxicities. Measurement of specific CD8+ T-cell responses by direct ex vivo ELISpot revealed a significant increase in interferon gamma–producing T cells against Melan-A (P = .015) after vaccination, but not against an Epstein-Barr virus control peptide (P = .86). There was a correlation between the magnitude of the increase in Melan-A–specific cells and clinical response (P = .046). Conclusion: This immunization approach may be more straightforward than dendritic cell strategies and seems to have clinical activity that can be correlated to a biologic end point.

scholarly journals Candida albicans and Candida parapsilosis Induce Different T-Cell Responses in Human Peripheral Blood Mononuclear Cells

2013 ◽  
Vol 208 (4) ◽  
pp. 690-698 ◽  
Author(s):  
Adél Tóth ◽  
Katalin Csonka ◽  
Cor Jacobs ◽  
Csaba Vágvölgyi ◽  
Joshua D. Nosanchuk ◽  
...  
Keyword(s):  
Candida Albicans ◽  
T Cell ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Candida Parapsilosis ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Cell Responses ◽  
Blood Mononuclear Cells
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