Efficient duplex solid-phase fluorescent assay (dFISA) for the simultaneous detection of specific anti-T. gondii IgG and IgM due to refined conjugates

2015 ◽  
Vol 420 ◽  
pp. 11-17 ◽  
Author(s):  
J.P. Rodrigues ◽  
H.F. Andrade
Keyword(s):  
Solid Phase ◽  
Simultaneous Detection ◽  
Fluorescent Assay

scholarly journals DETERMINATION OF SULFONAMIDES IN CHICKEN MEAT BY SOLID PHASE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

1970 ◽  
Vol 63 ◽  
Author(s):  
C. T. Matea ◽  
C. Bele ◽  
F. Dulf
Keyword(s):  
High Performance ◽  
Ammonium Acetate ◽  
Solid Phase ◽  
Simultaneous Detection ◽  
Gradient Elution ◽  
Normal Phase ◽  
Chicken Meat ◽  
Liquid Liquid Extraction ◽  
Halogenated Solvents

This paper describes a method for the simultaneous detection and quantification of six sul-fonamides in chicken meat using normal phase cartridge clean-up and HPLC with UV detection . A liquid – liquid extraction and Sep- Pak silica clean-up procedure which minimizes the presence of halogenated solvents was used for sample preparation . The HPLC determination was performed using a RP C 18 column and sulfonamides were detected at 266 nm. Mobile phase was 0.01 M ammonium acetate pH 4.6 ( A ) and methanol ( B). Chromatographic separation was obtained by gradient elution ( 22 % B to 50 % within 17 min , back to 22 % in 2 min, equilibration for 5 min).Average recoveries of analytes from spiked meat were higher than 74 % .

Simultaneous detection of fifteen biogenic amines in animal derived products by HPLC-FLD with solid-phase extraction after derivatization with dansyl chloride

2016 ◽  
Vol 8 (18) ◽  
pp. 3747-3755 ◽  
Author(s):  
Huaping Zhu ◽  
Shanshan Yang ◽  
Yan Zhang ◽  
Guozhen Fang ◽  
Shuo Wang
Keyword(s):  
Solid Phase Extraction ◽  
Biogenic Amines ◽  
Solid Phase ◽  
Simultaneous Detection ◽  
Dansyl Chloride ◽  
Phase Extraction

After extraction, derivatization and purification, fifteen kinds of biogenic amines in meat were separated and quantitated by HPLC-FLD.

A simple solid phase, peptide-based fluorescent assay for the efficient and universal screening of HRV 3C protease inhibitors

2012 ◽  
Vol 22 (15) ◽  
pp. 5018-5024 ◽  
Author(s):  
Katrin Schünemann ◽  
Stephen Connelly ◽  
Renata Kowalczyk ◽  
Jonathan Sperry ◽  
Ian A. Wilson ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Universal Screening ◽  
Solid Phase ◽  
Fluorescent Assay ◽  
3C Protease

Determination of PCB Residues in Vegetables by GC-MS

2012 ◽  
Vol 487 ◽  
pp. 724-729 ◽  
Author(s):  
Tie Bing Liu ◽  
Hai Yun Tu ◽  
Li Ming Han ◽  
Qiu Ping Yuan ◽  
Greg Duns
Keyword(s):  
High Speed ◽  
Polychlorinated Biphenyl ◽  
Solid Phase ◽  
Simultaneous Detection ◽  
Qualitative And Quantitative ◽  
Relative Standard ◽  
Combination Of Methods ◽  
Linear Concentration Range ◽  
Linear Concentration

This paper examines a method for the simultaneous detection of 12 different dioxin-like polychlorinated biphenyl (PCB) residues in vegetables. A qualitative and quantitative determination of PCB residues in vegetables was undertaken by a combination of high-speed freezing centrifugation, solid phase extraction, purification, concentration, and GC- MS and GC-ECD. From this combination of methods, 12 different PCBs were detected in vegetables over a linear concentration range of 0.001 to 2 µ g/L (ppb), with detection limits ranging from 0.4ppb to 0.5 ppb, a linear correlation coefficient of 0.99, recoveries ranging from 96.25%-117.5%, and a relative standard deviation ≤ 21%(RSD) .

scholarly journals New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 406 ◽  
Author(s):  
Leticia Díez-Quijada ◽  
Remedios Guzmán-Guillén ◽  
Ana Prieto Ortega ◽  
María Llana-Ruíz-Cabello ◽  
Alexandre Campos ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Simultaneous Detection ◽  
Ultra Performance Liquid Chromatography ◽  
Limit Of Quantification ◽  
Worldwide Distribution ◽  
Exposure Estimation ◽  
Liquid Chromatography Tandem Mass

Cyanotoxins are a large group of noxious metabolites with different chemical structure and mechanisms of action, with a worldwide distribution, producing effects in animals, humans, and crop plants. When cyanotoxin-contaminated waters are used for the irrigation of edible vegetables, humans can be in contact with these toxins through the food chain. In this work, a method for the simultaneous detection of Microcystin-LR (MC-LR), Microcystin-RR (MC-RR), Microcystin-YR (MC-YR), and Cylindrospermopsin (CYN) in lettuce has been optimized and validated, using a dual solid phase extraction (SPE) system for toxin extraction and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for analysis. Results showed linear ranges (5–50 ng g−1 f.w.), low values for limit of detection (LOD) (0.06–0.42 ng g−1 f.w.), and limit of quantification (LOQ) (0.16–0.91 ng g−1 f.w.), acceptable recoveries (41–93%), and %RSDIP values for the four toxins. The method proved to be robust for the three variables tested. Finally, it was successfully applied to detect these cyanotoxins in edible vegetables exposed to cyanobacterial extracts under laboratory conditions, and it could be useful for monitoring these toxins in edible vegetables for better exposure estimation in terms of risk assessment.

scholarly journals Simultaneous detection of three antineoplastic drugs on gloves by liquid chromatography with diode array detector

2010 ◽  
Vol 46 (4) ◽  
pp. 731-740 ◽  
Author(s):  
Adélia Maria Pimenta de Pádua Alcântara ◽  
Ricardo Vilela Vitor ◽  
Elisabeth Pizzamiglio Vieira ◽  
Isarita Martins
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase ◽  
Simultaneous Detection ◽  
Array Detector ◽  
Diode Array ◽  
Antineoplastic Drugs ◽  
Diode Array Detector ◽  
Care Workers ◽  
Prolonged Contact ◽  
Water Ph

The aim of this study was to develop a method for simultaneous detection of antineoplastic drugs on gloves since, in occupational exposure, the main contamination route is through dermal contact, which may occur via prolonged contact with contaminated surfaces or materials. The assay was performed by liquid chromatography using the following conditions for the detection of 5-fluorouracil (5-FU), methotrexate (MTX) and paclitaxel (TAX): diode array detection and UV quantification at 195 nm for TAX, at 265 nm for 5-FU and at 302 nm for MTX; ODS column (250 x 4 mm, 5 μm) with a similar guard column; mobile phase consisted of water (pH 4)-methanol-acetonitrile (35:15:50, v/v/v) with a flow rate of 1 mL min-1. The method presented a linear range from 0.25 to 20 μg mL-1 with r² higher than 0.99. Repeatability was <15% and satisfactory extraction efficiency was obtained when liquid-liquid extraction with ethyl acetate was used for 5-FU and TAX. Satisfactory solid phase extraction was also achieved with C18 cartridges and elution with methanol for MTX. The diode array detector allowed drug quantification at a concentration > 0.25 μg mL-1 in samples, although detection was possible in samples that presented values of around 0.1 μg mL-1. The results obtained suggest that the method developed can be applied for the simultaneous determination of the drugs studied and can be considered useful in exposure assessment for health care workers.

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