A novel real-time PCR approach for detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) in the freshwater crayfish Procambarus clarkii

2018 ◽  
Vol 157 ◽  
pp. 100-103 ◽  
Author(s):  
Bo-Kun Chen ◽  
Zhen Dong ◽  
Neng-Yuan Pang ◽  
Yan-Ying Nian ◽  
Dong-Chun Yan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Procambarus Clarkii ◽  
Freshwater Crayfish ◽  
Necrosis Virus

Iinfectious hypodermal and haematopoietic necrosis virus (IHHNV) infection in freshwater crayfish Procambarus clarkii

Aquaculture ◽  
2017 ◽  
Vol 477 ◽  
pp. 76-79 ◽  
Author(s):  
Bo-Kun Chen ◽  
Zhen Dong ◽  
Da-Peng Liu ◽  
Yong-Bin Yan ◽  
Neng-Yuan Pang ◽  
...  
Keyword(s):  
Procambarus Clarkii ◽  
Freshwater Crayfish ◽  
Necrosis Virus

Development of a sensitive and quantitative diagnostic assay for fish nervous necrosis virus based on two-target real-time PCR

2005 ◽  
Vol 110 (3-4) ◽  
pp. 167-179 ◽  
Author(s):  
L DALLAVALLE ◽  
V TOFFOLO ◽  
M LAMPRECHT ◽  
C MALTESE ◽  
G BOVO ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Nervous Necrosis Virus ◽  
Diagnostic Assay ◽  
Necrosis Virus

scholarly journals Detection of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV, Decapod Penstylhamaparvovirus 1) in Commodity Red Claw Crayfish (Cherax quadricarinatus) Imported into South Korea

2021 ◽  
Vol 9 (8) ◽  
pp. 856
Author(s):  
Chorong Lee ◽  
Seong-Kyoon Choi ◽  
Hye Jin Jeon ◽  
Seung Ho Lee ◽  
Young Kyoon Kim ◽  
...  
Keyword(s):  
South Korea ◽  
Procambarus Clarkii ◽  
Penaeid Shrimp ◽  
Freshwater Crayfish ◽  
Asian Countries ◽  
Necrosis Virus ◽  
Tissue Samples ◽  
Cherax Quadricarinatus ◽  
Pathogenic Viruses ◽  
Red Claw Crayfish

Freshwater crayfish, which are cultivated in aquaculture, are economically important for food and ornamental purposes. However, relatively few studies have focused on potentially pathogenic viruses in crayfish compared to in penaeid shrimp. Commodity red claw crayfish (Cherax quadricarinatus; 400 crayfish in 10 batches) and red swamp crayfish (Procambarus clarkii; 40 crayfish in 2 batches) imported into South Korea from Indonesia and China were screened by PCR to detect infectious hypodermal and hematopoietic necrosis virus (IHHNV or Decapod penstylhamaparvovirus 1). IHHNV was detected in tissue samples pooled from nine out of ten batches of red claw crayfish imported from Indonesia. Phylogenetic analysis of PCR amplicons from representative pools clustered the IHHNV strain with infectious-type II sequences commonly detected in Southeast Asian countries rather than with type III strains detected previously in whiteleg shrimp (Penaeus vannamei) cultured in South Korea. IHHNV DNA was detected most frequently in the muscle (eight batches, 66.7% samples), followed by in the hepatopancreas (five batches, 41.7% samples) and gills tissue (three batches, 25.0% samples). These data suggest that red claw crayfish could be a potential carrier of the virus and that quarantine procedures must be strengthened in South Korea to avoid importing infectious types of IHHNV in commodity crustaceans such as red claw crayfish.

Application and development of a TaqMan real-time PCR for detecting infectious spleen and kidney necrosis virus in Siniperca chuatsi

2017 ◽  
Vol 107 ◽  
pp. 98-105 ◽  
Author(s):  
Qiang Lin ◽  
Xiaozhe Fu ◽  
Lihui Liu ◽  
Hongru Liang ◽  
Huizhi Guo ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Necrosis Virus ◽  
Siniperca Chuatsi

scholarly journals Identification of the major capsid protein of erythrocytic necrosis virus (ENV) and development of quantitative real-time PCR assays for quantification of ENV DNA

2016 ◽  
Vol 28 (4) ◽  
pp. 382-391 ◽  
Author(s):  
Maureen K. Purcell ◽  
Schuyler Pearman-Gillman ◽  
Rachel L. Thompson ◽  
Jacob L. Gregg ◽  
Lucas M. Hart ◽  
...  
Keyword(s):  
Real Time ◽  
Capsid Protein ◽  
Real Time Pcr ◽  
Major Capsid Protein ◽  
Quantitative Real Time Pcr ◽  
Necrosis Virus ◽  
Pcr Assays

scholarly journals Universal reverse-transcriptase real-time PCR for infectious hematopoietic necrosis virus (IHNV)

2013 ◽  
Vol 106 (2) ◽  
pp. 103-115 ◽  
Author(s):  
MK Purcell ◽  
RL Thompson ◽  
KA Garver ◽  
LM Hawley ◽  
WN Batts ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Real Time Pcr ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Necrosis Virus ◽  
Infectious Hematopoietic Necrosis

scholarly journals ETEKSI WSSV (WHITE SPOT SYNDROM VIRUS) PADA LOBSTER AIR TAWAR (PROCAMBARUS CLARKII) MENGGUNAKAN METODE REAL TIME-PCR

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Nefi Andriana ◽  
Muhamad Ali ◽  
Sulaiman N. Depamede
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
White Spot Syndrome Virus ◽  
Procambarus Clarkii ◽  
White Spot ◽  
Rt Pcr ◽  
Chain Reaction ◽  
White Spot Syndrome

Serangan virus White Spot Syndrome (WSSV) telah memberikan dampak finansial yang cukup besar sejak tahun 1992. Tingkat mortalitas yang mencapai 100% menyebabkan banyak pembudidaya udang dan lobster menderita kerugian. Untuk mengatasi permasalahan tersebut sangat diperlukan informasi tentang cara penyebaran virus WSSV sehingga dapat diketahui solusi yang tepat untuk menghentikan penyebarannya pada tambak-tambak udang maupun lobster. Pada penelitian ini, Lobster Air Tawar yang dibudidayakan di BBI Aik Bukak dijadikan kontrol negatif (belum terinfeksi virus WSSV). Hasil penelitian ini menunjukkan bahwa penularan virus WSSV pada lobster air tawar dapat berlangsung melalui konsumsi udang yang telah terinfeksi virus WSSV serta melalui aliran air. Pleopod atau kaki renang yang dideteksi dengan teknologi Real Time Polimerase Chain Reaction (RT-PCR) mengandung virus WSSV yang lebih dominan dibandingkan insangKata kunci: White Spot Syndrome Virus, Lobster Air Tawar, RT-PCR

scholarly journals Development of Real-Time Polymerase Chain Reaction Assay with TaqMan Probe for the Quantitative Detection of Infectious Hypodermal and Hematopoietic Necrosis Virus from Shrimp

2006 ◽  
Vol 89 (1) ◽  
pp. 240-244 ◽  
Author(s):  
Zhi-Qin Yue ◽  
Hong Liu ◽  
Wei-Ji Wang ◽  
Zhi-Wen Lei ◽  
Cheng-Zhu Liang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Real Time Pcr ◽  
Taqman Probe ◽  
Quantitative Detection ◽  
Necrosis Virus ◽  
Chain Reaction ◽  
Pcr Assay ◽  
The Real ◽  
Polymerase Chain

Abstract An assay was developed for the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) based on real-time quantitative polymerase chain reaction (PCR). A pair of primers and a TaqMan probe were designed that are specific for the recognition of a conservative region in the IHHNV genome. The IHHNV real-time PCR assay had a detection limit of 9 DNA copies,with a dynamic range of detection between 9 106 and 9 DNA copies. The primer pairs and probe were specific to IHHNV and did not cross-reactwith shrimp genomic DNAor other shrimp viruses such as White Spot Syndrome Virus (WSSV), Monodon Baculovirus (MBV), and hepatopancreatic parvovirus (HPV). This assay has a broad application for basic and clinical investigations. For clinical samples, the real-time PCR assay detected all the positive samples screened by conventional PCR, which indicated the sensitivity of the real-time assay. The IHHNV real-time PCR assay with high sensitivity, specificity, wide range of detection ability, and simplicity is particularly useful for screening large numbers of specimens and measuring viral loads to monitor the broodstock.

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