In vitro dentine tubule occlusion by a novel toothpaste containing calcium silicate and sodium phosphate

Abstract Objectives This study aimed to evaluate the effect of in-office bleaching on the enamel surface and the efficacy of calcium silicate-sodium phosphate-fluoride salt (CS) and NovaMin bioactive glass (NM) dentifrice in remineralizing bleached enamel. Materials and Methods Forty extracted premolars were sectioned mesio-distally, and the facial and lingual enamel were flattened and polished. The samples were equally divided into nonbleached and bleached with 38% hydrogen peroxide (HP). Each group was further divided according to the remineralization protocol (n = 10); no remineralization treatment (nontreated), CS, or NM, applied for 3 minutes two times/day for 7 days, or CS combined with NR-5 boosting serum (CS+NR-5) applied for 3 minutes once/day for 3 days. The average Knoop hardness number (KHN) and surface roughness (utilizing atomic force microscopy) were measured. Surface topography/elemental analysis was analyzed by using scanning electron microscopy/energy dispersive X-ray analysis. All the tests were performed at baseline, after bleaching, and following each remineralization protocol. Data were statistically analyzed by two-way analysis of variance and Bonferroni post hoc multiple comparison tests (α = 0.05). Results HP significantly reduced KHN and increased roughness (p < 0.05). All remineralization materials increased the hardness and reduced the surface roughness after bleaching except NM, which demonstrated significantly increased roughness (p < 0.05). Ca/P ratio decreased after bleaching (p < 0.05), and following treatment, CS and CS+NR-5 exhibited higher remineralization capacity in comparison to NM (p < 0.05). Conclusion Although none of the material tested was able to reverse the negative effect of high-concentration in-office HP on enamel completely, the remineralization efficacy of CS and CS+NR-5 was superior to that of NM.

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In vitro remineralization of enamel subsurface lesions and assessment of dentine tubule occlusion from NaF dentifrices with and without calcium

Journal of Indian Society of Pedodontics and Preventive Dentistry ◽

10.4103/0970-4388.112403 ◽

2013 ◽

Vol 31 (1) ◽

pp. 29 ◽

Cited By ~ 15

Author(s):

AR Prabhakar ◽

AJaiswal Manojkumar ◽

N Basappa

Keyword(s):

Dentine Tubule ◽

Tubule Occlusion

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Effect of Citric Acid on Color Changes of Calcium Silicate-Based Cements an In Vitro Study

Applied Sciences ◽

10.3390/app11052339 ◽

2021 ◽

Vol 11 (5) ◽

pp. 2339

Author(s):

Joanna Metlerska ◽

Till Dammaschke ◽

Mariusz Lipski ◽

Irini Fagogeni ◽

Anna Machoy-Mokrzyńska ◽

...

Keyword(s):

Citric Acid ◽

Calcium Silicate ◽

In Vitro Study ◽

Vitro Study ◽

Color Measurement ◽

Color Changes ◽

Naked Eye ◽

Root Canal Irrigants ◽

The Difference

The aim of the present in vitro study was to investigate the effects of 10% and 40% citric acid (CA) on the color of calcium silicate–based cements (CSCs) in comparison to the effects of common root canal irrigants. Samples of six CSCs (n = 6)—ProRoot MTA (Dentsply, Tulsa, OK, USA), Biodentine (Septodont, Saint-Maur-des-Fossés, France), MTA Plus (Avalon Biomed Inc, by Prevest Denpro Limited, Jammu, India), MTA Repair HP (Angelus, Londrina, PR, Brazil), Ortho MTA (BioMTA, Seoul, Korea), and Retro MTA (BioMTA, Seoul, Korea)—were immersed in 10% and 40% CA as well as 15% EDTA, 2% NaOCl, 2% CHX, and 0.9% NaCl for 15 min, 1 h, and 24 h. ΔE values, representing the difference between the final and baseline values of the color components, were then determined using a VITA Easyshade Compact 5.0 spectrophotometer. Naked-eye evaluation of the changes in color and structures of the materials was performed using our own scale. Upon immersion of the materials in both 10% and 40% CA, there were statistically significant differences between spectrophotometric color measurement results for all CSCs (P < 0.05). However, CA does not cause dark discoloration, observable with the naked eye, of any of the materials, such as NaOCl and CHX. Significant statistical differences were also found between all CSCs in terms of submersion duration (P < 0.05). CA, which could be an alternative to EDTA use, caused greater CSCs discoloration and changed some of their structures. Unless required by the therapeutic procedure, clinicians should pay attention to the fact that the irrigant may affect the CSCs discoloration and minimize the contact time of irrigant with CSCs.

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Tooth discoloration induced by apical plugs with hydraulic calcium silicate-based cements in teeth with open apices—a 2-year in vitro study

Clinical Oral Investigations ◽

10.1007/s00784-021-04009-0 ◽

2021 ◽

Author(s):

Ralf Krug ◽

C. Ortmann ◽

S. Reich ◽

B. Hahn ◽

G. Krastl ◽

...

Keyword(s):

Calcium Silicate ◽

In Vitro Study ◽

Color Difference ◽

Blood Contamination ◽

Tooth Color ◽

Color Changes ◽

Gutta Percha ◽

Tooth Discoloration ◽

Post Hoc

Abstract Objectives To assess tooth discoloration induced by different hydraulic calcium silicate-based cements (HCSCs), including effects of blood and placement method. Materials and methods Eighty bovine teeth cut to a length of 18 mm (crown 8 mm, root 10 mm) were randomly assigned to 10 groups (n = 8), receiving orthograde apical plug treatment (APT). Apical plugs were 4 mm in length and made of ProRoot MTA (Dentsply), Medcem MTA (Medcem), TotalFill BC RRM Fast Set Putty (Brasseler), or Medcem Medical Portland Cement (Medcem) plus bismuth oxide (Bi2O3) with and without bovine blood. Further, orthograde (with or without preoperative adhesive coronal dentin sealing) and retrograde APT were compared. Teeth were obturated with gutta-percha and sealer, sealed with composite and stored in distilled water. Tooth color was measured on apical plug, gutta-percha/sealer, and crown surface before treatment versus 24 h, 1, 3, 6, 12, and 24 months after treatment by spectrophotometry. Color difference (ΔE) values were calculated and analyzed by Shapiro–Wilk test, ANOVA with post hoc tests, Friedman test, t test, and post hoc tests with Bonferroni correction (α = .05). Results Tooth discoloration occurred in all groups with no significant differences between HCSCs (p > .05). After 24 months, color changes were prominent on roots but insignificant on crowns. Blood contamination induced a significantly decreased luminescence (p < .05). Blood had a stronger impact on tooth color than Bi2O3. No relevant effects of retrograde placement (p > .05) or preoperative dentin sealing (p > .05) were detected. Conclusions Apical plugs of the tested HCSCs cause discoloration of bovine roots, but not discoloration of bovine tooth crowns within a 24-month period. Clinical relevance APT should be performed carefully while avoiding direct contact with the coronal dentin, and in that case no aesthetic impairments occur.

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