scholarly journals Abca8b promotes myelination via regulation of chondroitin sulfate proteoglycan 4 localization in oligodendrocyte precursor cells in mice

2021 ◽  
pp. 100147
Author(s):  
Yiran Liu ◽  
David Castano ◽  
Francesco Girolamo ◽  
Laia Trigueros-Motos ◽  
Han-Gyu Bae ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Precursor Cells ◽  
Chondroitin Sulfate Proteoglycan ◽  
Oligodendrocyte Precursor Cells ◽  
Sulfate Proteoglycan ◽  
Proteoglycan 4 ◽  
Oligodendrocyte Precursor

scholarly journals Chondroitin Sulfate Proteoglycan 4 and Its Potential As an Antibody Immunotherapy Target across Different Tumor Types

2018 ◽  
Vol 8 ◽  
Author(s):  
Kristina M. Ilieva ◽  
Anthony Cheung ◽  
Silvia Mele ◽  
Giulia Chiaruttini ◽  
Silvia Crescioli ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Proteoglycan 4 ◽  
Antibody Immunotherapy ◽  
Immunotherapy Target ◽  
Tumor Types

Roles of chondroitin sulfate proteoglycan 4 in fibrogenic/adipogenic differentiation in skeletal muscle tissues

2016 ◽  
Vol 347 (2) ◽  
pp. 367-377 ◽  
Author(s):  
Shiho Takeuchi ◽  
Shin-Ichi Nakano ◽  
Katsuyuki Nakamura ◽  
Atsufumi Ozoe ◽  
Peggie Chien ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Chondroitin Sulfate ◽  
Adipogenic Differentiation ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Muscle Tissues ◽  
Proteoglycan 4

scholarly journals A chondroitin sulfate proteoglycan 4‑specific monoclonal antibody inhibits melanoma cell invasion in a spheroid model

2021 ◽  
Vol 59 (3) ◽  
Author(s):  
Karolina Uranowska ◽  
Mahzeiar Samadaei ◽  
Tanja Kalic ◽  
Matthias Pinter ◽  
Heimo Breiteneder ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Chondroitin Sulfate ◽  
Melanoma Cell ◽  
Cell Invasion ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Specific Monoclonal Antibody ◽  
Proteoglycan 4

scholarly journals Receptor Binding Domains of TcdB from Clostridioides difficile for Chondroitin Sulfate Proteoglycan-4 and Frizzled Proteins Are Functionally Independent and Additive

Toxins ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 736
Author(s):  
Daniel Henkel ◽  
Helma Tatge ◽  
Dennis Schöttelndreier ◽  
Liang Tao ◽  
Min Dong ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Cancer Cell Line ◽  
Cell Types ◽  
Chondroitin Sulfate Proteoglycan ◽  
Cervical Cancer Cell Line ◽  
Sulfate Proteoglycan ◽  
Clostridioides Difficile ◽  
Cell Line Hela ◽  
Proteoglycan 4 ◽  
The Impact

Toxin B (TcdB) produced by Clostridioides difficile is a main pathogenicity factor that affects a variety of different cell types within the colonic mucosa. TcdB is known to utilize frizzled-1,2,7 and chondroitin sulfate proteoglycan-4 (CSPG4) as protein receptors. By using human cervical cancer cell line HeLa CSPG4 knockout (CSPG4−/−) cells as well as TcdB mutants which do not bind to either CSPG4 or frizzled-1,2,7, or both, we evaluated the impact of the individual receptors for cytopathic and cytotoxic effects of TcdB. We compared TcdB from the reference strain VPI10463 (TcdBVPI) and the endemic strain R20291 (TcdBR20) which does not interact with frizzled-1,2,7. TcdBVPI devoid of CSPG4 binding (TcdBVPI ΔCROP) shows identical cytopathic potency as full-length TcdB in HeLa CSPG4−/− cells, indicating that interaction with frizzled proteins is not affected in the presence of the C-terminal CROP domain. We validated CSPG4 as cellular receptor for both TcdB toxinotypes in HeLa and HEp-2 cells. By exchange of a single phenylalanine residue, 1597 with serine, we generated a mutated TcdBVPI variant (TcdBVPI F1597S) that in accordance with TcdBR20 lacks binding to frizzled-1,2,7 and showed identical potency as TcdBR20 on HeLa cells. This enabled us to estimate the respective share of CSPG4 and frizzled-1,2,7 in the cytotoxic and cytopathic effect induced by TcdB. Our data reveal that binding to frizzled-1,2,7 and to CSPG4 occurs independently and in an additive manner.

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