Fluorescence quenching effect of guanine interacting with water-soluble cationic porphyrin

2014 ◽  
Vol 147 ◽  
pp. 27-33 ◽  
Author(s):  
Magdalena Makarska-Bialokoz
Keyword(s):  
Fluorescence Quenching ◽  
Water Soluble ◽  
Cationic Porphyrin ◽  
Quenching Effect

scholarly journals A water-soluble fluorescent chemosensor based on Asp functionalized naphthalimide for successive detection Fe3+ and H2PO4−

2018 ◽  
Vol 96 (4) ◽  
pp. 363-370 ◽  
Author(s):  
You-Ming Zhang ◽  
Xiao-Peng Chen ◽  
Guo-Yan Liang ◽  
Kai-Peng Zhong ◽  
Hong Yao ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Metal Ions ◽  
Aspartic Acid ◽  
High Selectivity ◽  
Fluorescent Sensor ◽  
Water Solution ◽  
Water Soluble ◽  
Selective Recognition ◽  
Actual Usage ◽  
Quenching Process

The selective recognition of target ions in water is very important and the development of novel water-soluble chemosensor is still an intriguing challenge. Herein, a novel water-soluble fluorescent sensor based on aspartic acid (Asp) functionalized 1,8-naphthalimide derivative (Asp-NI) has been designed and synthesized. The sensor Asp-NI could dissolve in water and successively detect Fe3+ and H2PO4− in water solution with high selectivity and sensitivity. The detection limits are 4.97 × 10−7 mol/L for Fe3+ and 5.27 × 10−6 mol/L for H2PO4−. Other coexistent competitive metal ions (Hg2+, Ag+, Ca2+, Cu2+, Co2+, Ni2+, Cd2+, Pb2+, Zn2+, Cr3+, and Mg2+) showed no interference in the Fe3+ detection process. The sensor Asp-NI could act as a Fe3+ and H2PO4− controlled “On–Off–On” fluorescent switch. More interestingly, the Fe3+ induced fluorescence quenching process could be totally reversed by the addition of H2PO4−, this “On–Off–On” switching process could be repeated several times with little fluorescence loss. Notably, the actual usage of sensor Asp-NI was further demonstrated by test kits.

scholarly journals Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test

2016 ◽  
Vol 14 (1) ◽  
pp. 258-266 ◽  
Author(s):  
Sayed M Derayea ◽  
Mahmoud A Omar ◽  
Mohamed Aboel-Kasem Abdel-Lateef ◽  
Ahmed I. Hassan
Keyword(s):  
Fluorescence Quenching ◽  
Beta Blockers ◽  
Dosage Forms ◽  
Ion Pair ◽  
Content Uniformity ◽  
Eosin Y ◽  
Pharmaceutical Dosage Forms ◽  
Quenching Effect ◽  
Spectrofluorimetric Method

AbstractA simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method.

A simple enzyme-catalyzed reaction induced “switch” type fluorescence biosensor based on carbon nitride nanosheets for the assay of alkaline phosphatase activity

The Analyst ◽  
2020 ◽  
Vol 145 (19) ◽  
pp. 6277-6282
Author(s):  
Xi Zhu ◽  
Huifeng Xu ◽  
Yan Zhan ◽  
Wenjing Li ◽  
Yongqiang Dong ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Fluorescence Quenching ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Carbon Nitride ◽  
Quenching Effect ◽  
Fluorescence Switch ◽  
Switch Type ◽  
Fluorescence Biosensor ◽  
Carbon Nitride Nanosheets

An enzyme-catalyzed fluorescence “switch” type sensor was constructed for assay of alkaline phosphatase activity by combining the fluorescence quenching effect of Ag+ on ultrathin g-C3N4 nanosheets with the simple redox reaction of AA and Ag+.

Photoactive rare earth complexes for fluorescence tuning and sensing cations (Fe3+) and anions (Cr2O72−)

RSC Advances ◽  
2015 ◽  
Vol 5 (9) ◽  
pp. 6752-6757 ◽  
Author(s):  
Xiang Shen ◽  
Bing Yan
Keyword(s):  
Rare Earth ◽  
Coordination Polymer ◽  
Fluorescence Quenching ◽  
Polymer Materials ◽  
Rare Earth Complexes ◽  
Quenching Effect ◽  
Rare Earth Coordination

A series of photoactive rare earth coordination polymer materials RE/Ad/BPDC (Ad = adeninate, BPDC = biphenyl-4,4′-dicarboxylate) with tunable multi-color luminescence are synthesized. Y0.99Eu0.01/Ad/BPDC has a selective fluorescence quenching effect for Fe3+ and Cr2O72−.

Fluorescence quenching effect of substituted tetraphenylporphyrins

1981 ◽  
Vol 15 (2) ◽  
pp. 163-167 ◽  
Author(s):  
Anthony Harriman ◽  
R.John Hosie
Keyword(s):  
Fluorescence Quenching ◽  
Quenching Effect

scholarly journals Spectroscopic Studies on the Interaction of a Water-Soluble Cationic Porphyrin with Bovine Serum Albumin

2013 ◽  
Vol 36 (1-2) ◽  
pp. 21-26 ◽  
Author(s):  
Hamid Dezhampanah ◽  
Abdol-Khalegh Bordbar ◽  
Yadolahe Khodadusdt
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Molecular Conformation ◽  
Fluorescence Emission ◽  
Water Soluble ◽  
Aggregate Formation ◽  
Cationic Porphyrin ◽  
Porphyrin Complex ◽  
Induced Aggregation

The interaction of a water-soluble cationic porphyrin, Cobalt(III) 5, 10, 15, 20-tetrakis (1-methylpyridinium-4-yl) porphyrin [Co(III)TMPyP], with bovine serum albumin (BSA) has been studied in 1 mM phosphate buffer pH 7.0 containing 5 mM NaCl by UV-vis absorption, resonance light scattering (RLS) and fluorescence spectroscopies at 25°C. The results of RLS studies represent no aggregate formation of porphyrin in the surface of BSA and low tendency of this porphyrin for aggregate formation.The binding of porphyrin complex to BSA quenches fluorescence emission of BSA via a dynamic mechanism and the quenching process obeys a linear Stern-Volmer relationship. The values of Stern-Volmer constants, KSV, was determined nearly 105M−1, that depend on BSA concentration. The average aggregation number of BSA calculated from the analysis of fluorescence quenching data indicates that absence of any porphyrin induced aggregation of BSA due to its interaction with porphyrin complex. The binding of Co(III) TMPyP had no obvious effect on the molecular conformation of the protein. Electrostatic force played an important role in the binding due to the opposite charges on porphyrin and the protein.

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