scholarly journals Cancer Cell Mechanobiology: A New Frontier for Cancer Research

2021 ◽  
Author(s):  
Weibo Yu ◽  
Shivani Sharma ◽  
Elizabeth Rao ◽  
Amy C. Rowat ◽  
James K. Gimzewski ◽  
...  
Keyword(s):  
Cancer Research ◽  
Cancer Cell ◽  
New Frontier

scholarly journals A biochemical comparison of the lung, colonic, brain, renal, and ovarian cancer cell lines using 1H-NMR spectroscopy

2020 ◽  
Vol 40 (4) ◽  
Author(s):  
Cong Hu ◽  
Zhigang Liu ◽  
Hailin Zhao ◽  
Lingzhi Wu ◽  
Qingquan Lian ◽  
...  
Keyword(s):  
Cancer Research ◽  
Nmr Spectroscopy ◽  
Cell Lines ◽  
Cancer Cell ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Cancer Cell Lines ◽  
Media Analysis ◽  
Metabolic Phenotyping ◽  
Biochemical Comparison

Abstract Cancer cell lines are often used for cancer research. However, continuous genetic instability-induced heterogeneity of cell lines can hinder the reproducibility of cancer research. Molecular profiling approaches including transcriptomics, chromatin modification profiling, and proteomics are used to evaluate the phenotypic characteristics of cell lines. However, these do not reflect the metabolic function at the molecular level. Metabolic phenotyping is a powerful tool to profile the biochemical composition of cell lines. In the present study, 1H-NMR spectroscopy-based metabolic phenotyping was used to detect metabolic differences among five cancer cell lines, namely, lung (A549), colonic (Caco2), brain (H4), renal (RCC), and ovarian (SKOV3) cancer cells. The concentrations of choline, creatine, lactate, alanine, fumarate and succinate varied remarkably among different cell types. The significantly higher intracellular concentrations of glutathione, myo-inositol, and phosphocholine were found in the SKOV3 cell line relative to other cell lines. The concentration of glutamate was higher in both SKOV3 and RCC cells compared with other cell lines. For cell culture media analysis, isopropanol was found to be the highest in RCC media, followed by A549 and SKOV3 media, while acetone was the highest in A549, followed by RCC and SKOV3. These results demonstrated that 1H-NMR-based metabolic phenotyping approach allows us to characterize specific metabolic signatures of cancer cell lines and provides phenotypical information of cellular metabolism.

scholarly journals Susana Godinho: Placing cell biology at the center of cancer research

2020 ◽  
Vol 219 (2) ◽  
Author(s):  
Marie Anne O’Donnell
Keyword(s):  
Cancer Research ◽  
Cancer Cell ◽  
Cell Biology

Godinho investigates the role centrosomes play in cancer cell biology.

scholarly journals Comprehensive transcriptomic analysis of cell lines as models of primary tumor samples across 22 tumor types

2018 ◽  
Author(s):  
K. Yu ◽  
B. Chen ◽  
D. Aran ◽  
J. Charalel ◽  
A. Butte ◽  
...  
Keyword(s):  
Cancer Research ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Primary Tumor ◽  
Cancer Biology ◽  
Cancer Cell Lines ◽  
Primary Tumors ◽  
Tumor Types

AbstractCancer cell lines are commonly used as models for cancer biology. While they are limited in their ability to capture complex interactions between tumors and their surrounding environment, they are a cornerstone of cancer research and many important findings have been discovered utilizing cell line models. Not all cell lines are appropriate models of primary tumors, however, which may contribute to the difficulty in translating in vitro findings to patients. Previous studies have leveraged public datasets to evaluate cell lines as models of primary tumors, but they have been limited in scope to specific tumor types and typically ignore the presence of tumor infiltrating cells in the primary tumor samples. We present here a comprehensive pan-cancer analysis utilizing approximately 9,000 transcriptomic profiles from The Cancer Genome Atlas and the Cancer Cell Line Encyclopedia to evaluate cell lines as models of primary tumors across 22 different tumor types. After adjusting for tumor purity in the primary tumor samples, we performed correlation analysis and differential gene expression analysis between the primary tumor samples and cell lines. We found that cell-cycle pathways are consistently upregulated in cell lines, while no pathways are consistently upregulated across the primary tumor samples. In a case study, we compared colorectal cancer cell lines with primary tumor samples across the colorectal subtypes and identified three colorectal cell lines that were derived from fibroblasts rather than tumor epithelial cells. Lastly, we propose a new set of cell lines panel, the TCGA-110, which contains the most representative cell lines from 22 different tumor types as a more comprehensive and informative alternative to the NCI-60 panel. Our analysis of the other tumor types are available in our web app (http://comphealth.ucsf.edu/TCGA110) as a resource to the cancer research community, and we hope it will allow researchers to select more appropriate cell line models and increase the translatability of in vitro findings.

scholarly journals Decellularized Extracellular Matrix for Cancer Research

Materials ◽  
2019 ◽  
Vol 12 (8) ◽  
pp. 1311 ◽  
Author(s):  
Takashi Hoshiba
Keyword(s):  
Cancer Research ◽  
Extracellular Matrix ◽  
Cancer Cell ◽  
Cancer Progression ◽  
Intracellular Signaling ◽  
Genetic Mutation ◽  
Cell Behavior ◽  
Characterization Methods ◽  
Decellularized Extracellular Matrix

Genetic mutation and alterations of intracellular signaling have been focused on to understand the mechanisms of oncogenesis and cancer progression. Currently, it is pointed out to consider cancer as tissues. The extracellular microenvironment, including the extracellular matrix (ECM), is important for the regulation of cancer cell behavior. To comprehensively investigate ECM roles in the regulation of cancer cell behavior, decellularized ECM (dECM) is now used as an in vitro ECM model. In this review, I classify dECM with respect to its sources and summarize the preparation and characterization methods for dECM. Additionally, the examples of cancer research using the dECM were introduced. Finally, future perspectives of cancer studies with dECM are described in the conclusions.

scholarly journals Genetically Engineered Neoplastic Cerebral Organoids – A New Frontier in Preclinical Cancer Research

Neurosurgery ◽  
2019 ◽  
Vol 85 (2) ◽  
pp. E172-E173
Author(s):  
Ryan Hess ◽  
Karl R Abi-Aad ◽  
Matthew E Welz ◽  
Bernard R Bendok
Keyword(s):  
Cancer Research ◽  
Genetically Engineered ◽  
New Frontier

scholarly journals Printing the Pathway Forward in Bone Metastatic Cancer Research: Applications of 3D Engineered Models and Bioprinted Scaffolds to Recapitulate the Bone–Tumor Niche

Cancers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 507
Author(s):  
Anne M. Hughes ◽  
Alexus D. Kolb ◽  
Alison B. Shupp ◽  
Kristy M. Shine ◽  
Karen M. Bussard
Keyword(s):  
Cancer Research ◽  
Tumor Cell ◽  
Cancer Cell ◽  
Bone Tumor ◽  
Metastatic Cancer ◽  
Critical Role ◽  
Three Dimensional ◽  
Biocompatible Materials

Breast cancer commonly metastasizes to bone, resulting in osteolytic lesions and poor patient quality of life. The bone extracellular matrix (ECM) plays a critical role in cancer cell metastasis by means of the physical and biochemical cues it provides to support cellular crosstalk. Current two-dimensional in-vitro models lack the spatial and biochemical complexities of the native ECM and do not fully recapitulate crosstalk that occurs between the tumor and endogenous stromal cells. Engineered models such as bone-on-a-chip, extramedullary bone, and bioreactors are presently used to model cellular crosstalk and bone–tumor cell interactions, but fall short of providing a bone-biomimetic microenvironment. Three-dimensional bioprinting allows for the deposition of biocompatible materials and living cells in complex architectures, as well as provides a means to better replicate biological tissue niches in-vitro. In cancer research specifically, 3D constructs have been instrumental in seminal work modeling cancer cell dissemination to bone and bone–tumor cell crosstalk in the skeleton. Furthermore, the use of biocompatible materials, such as hydroxyapatite, allows for printing of bone-like microenvironments with the ability to be implanted and studied in in-vivo animal models. Moreover, the use of bioprinted models could drive the development of novel cancer therapies and drug delivery vehicles.

scholarly journals Epigenomics: Pioneering a New Frontier in Cancer Research

2012 ◽  
Vol 03 (01) ◽  
Author(s):  
Yana Chervona ◽  
Max Costa ◽  
Wei Dai
Keyword(s):  
Cancer Research ◽  
New Frontier
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