scholarly journals Different doses of strontium ranelate and mechanical vibration modulate distinct responses in the articular cartilage of ovariectomized rats

2017 ◽  
Vol 25 (7) ◽  
pp. 1179-1188 ◽  
Author(s):  
A.G.H. Mierzwa ◽  
J.F. Campos ◽  
M.F. Jesus ◽  
H.B. Nader ◽  
M. Lazaretti-Castro ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Strontium Ranelate ◽  
Mechanical Vibration ◽  
Ovariectomized Rats ◽  
Different Doses

scholarly journals Cumulative Effects Of Strontium Ranelate and Impact Exercise On Bone Mass In Ovariectomized Rats

2021 ◽  
Vol 22 (6) ◽  
pp. 3040
Author(s):  
Priscilla Aveline ◽  
Annabelle Cesaro ◽  
Marija Mazor ◽  
Thomas M. Best ◽  
Eric Lespessailles ◽  
...  
Keyword(s):  
Bone Mineral ◽  
Fat Mass ◽  
Lipid Content ◽  
Strontium Ranelate ◽  
Bending Test ◽  
Ovariectomized Rats ◽  
Whole Body ◽  
Left Femur ◽  
Osteocyte Apoptosis ◽  
Ovx Rats

OBJECTIVE: To explore the effect of physical exercise (EXE), strontium ranelate (SR), or their combination on bone status in ovariectomized (OVX) rats. DESIGN: Sixty female Wistar rats were randomized to one of five groups: sham (Sh), OVX (O), OVX+EXE (OE), OVX+SR (OSR), and OVX+EXE+SR (OESR). Animals in EXE groups were subjected to 10 drops per day (45 cm in height); rats in SR groups received 625 mg/kg/day of SR, 5 days/week for 8 weeks. Bone mineral density (BMD) and bone mineral content (BMC, dual-energy X-ray absorptiometry (DXA)), mechanical strength of the left femur (three-point bending test), and femur microarchitecture of (micro-computed tomography imaging, microCT) analyses were performed to characterize biomechanical and trabecular/cortical structure. Bone remodeling, osteocyte apoptosis, and lipid content were evaluated by ELISA and immunofluorescence tests. RESULTS: In OVX rats, whole-body BMD, trabecular parameters, and osteocalcin (OCN) levels decreased, while weight, lean/fat mass, osteocyte apoptosis, and lipid content all increased. EXE after ovariectomy improved BMD and BMC, trabecular parameters, cross-sectional area (CSA), moment of inertia, and OCN levels while decreasing osteocyte apoptosis and lipid content. SR treatment increased BMD and BMC, trabecular parameters, CSA, stiffness, OCN, and alkaline phosphatase (ALP) levels. Furthermore, fat mass, N-telopeptide (NTX) level, osteocyte apoptosis, and lipid content significantly decreased. The combination of both EXE and SR improved bone parameters compared with EXE or SR alone. CONCLUSION: EXE and SR had positive and synergistic effects on bone formation and resorption.

Peri-Implant Osteogenesis Behavior in Ovariectomized Rats Treated with Strontium Ranelate

2017 ◽  
Vol 75 (10) ◽  
pp. e374-e375
Author(s):  
J.Z. Colete ◽  
G.A.C. Momesso ◽  
T.O.B. Polo ◽  
I.R. Garcia-Júnior ◽  
L.P. Faverani ◽  
...  
Keyword(s):  
Strontium Ranelate ◽  
Ovariectomized Rats

Combined effect of parathyroid hormone and strontium ranelate on bone healing in ovariectomized rats

Oral Diseases ◽  
2018 ◽  
Vol 24 (7) ◽  
pp. 1255-1269 ◽  
Author(s):  
Funda Göker ◽  
Selim Ersanlı ◽  
Volkan Arısan ◽  
Erdal Cevher ◽  
Emine Elif Güzel ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Healing ◽  
Strontium Ranelate ◽  
Combined Effect ◽  
Ovariectomized Rats

Effects of different doses of ferutinin on bone formation/resorption in ovariectomized rats

2012 ◽  
Vol 30 (6) ◽  
pp. 619-629 ◽  
Author(s):  
Francesco Cavani ◽  
Marzia Ferretti ◽  
Gianluca Carnevale ◽  
Laura Bertoni ◽  
Manuela Zavatti ◽  
...  
Keyword(s):  
Bone Formation ◽  
Ovariectomized Rats ◽  
Different Doses

Systemic treatment with strontium ranelate promotes tibial fracture healing in ovariectomized rats

2009 ◽  
Vol 21 (11) ◽  
pp. 1889-1897 ◽  
Author(s):  
Y. F. Li ◽  
E. Luo ◽  
G. Feng ◽  
S. S. Zhu ◽  
J. H. Li ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Strontium Ranelate ◽  
Tibial Fracture ◽  
Systemic Treatment ◽  
Ovariectomized Rats

scholarly journals The influence of long-term treadmill exercise on bone mass and articular cartilage in ovariectomized rats

2010 ◽  
Vol 11 (1) ◽  
Author(s):  
Ting-Kuo Chang ◽  
Chang-Hung Huang ◽  
Chun-Hsiung Huang ◽  
Hsuan-Chiang Chen ◽  
Cheng-Kung Cheng
Keyword(s):  
Articular Cartilage ◽  
Bone Mass ◽  
Treadmill Exercise ◽  
Ovariectomized Rats

Effects of strontium ranelate, raloxifene and misoprostol on bone mineral density in ovariectomized rats

2009 ◽  
Vol 147 (2) ◽  
pp. 192-194 ◽  
Author(s):  
Nefise Ahmet-Camcioglu ◽  
Tulay Okman-Kilic ◽  
Gulay Durmus-Altun ◽  
Galip Ekuklu ◽  
Mustafa Kucuk
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Strontium Ranelate ◽  
Ovariectomized Rats ◽  
Mineral Density

Strontium ranelate does not stimulate bone formation in ovariectomized rats

2008 ◽  
Vol 19 (9) ◽  
pp. 1331-1341 ◽  
Author(s):  
R. K. Fuchs ◽  
M. R. Allen ◽  
K. W. Condon ◽  
S. Reinwald ◽  
L. M. Miller ◽  
...  
Keyword(s):  
Bone Formation ◽  
Strontium Ranelate ◽  
Ovariectomized Rats ◽  
Stimulate Bone Formation

Effects of Mechanical Vibration on Matrix Production and Proliferation of Three-Dimensional Cultured Chondrocytes

2008 ◽  
Author(s):  
Yuta Takagi ◽  
Toshihiko Shiraishi ◽  
Shin Morishita ◽  
Ryohei Takeuchi ◽  
Tomoyuki Saito ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hyaluronic Acid ◽  
Articular Cartilage ◽  
Signaling Pathways ◽  
Weight Bearing ◽  
Mechanical Vibration ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Vibration Treatment ◽  
Matrix Production

This paper describes the effects of vibration stimulation on chondrocytes in three-dimensional culture in relation to the production of regenerative cartilage tissue, using collagen artificial skin as a carrier and supplementation with hyaluronic acid (used in the conservative treatment of osteoarthritis), and the mechanism of the adaptive response of chondrocytes to mechanical loading. The experimental condition imitates an environment of articular cartilage in vivo that chondrocytes are completely surrounded by the extracellular matrix and receives mechanical stimulation for the weight-bearing mechanics. Chondrocytes were isolated from articular cartilage of porcine metatarsophalangeal joints. Experiments were performed under four different culture conditions: control condition, in which chondrocytes were cultured with atelocollagen gel and collagen artificial skins, and no vibration (HA−Vib−); HA−Vib+, in which chondrocytes were cultured in atelocollagen gel and collagen artificial skins with vibration treatment for 2 weeks; HA+Vib−, in which chondrocytes were cultured in medium containing 0.1% hyaluronic acid; and HA+Vib+, in which chondrocytes were cultured in medium containing 0.1% hyaluronic acid with vibration treatment for 2 weeks. Histologic analysis was conducted at 14 days of culture. The proliferation of chondrocytes was obtained by counting the number of cells with a hemocytometer after 3, 7, 10, and 14 days of culture. The expression of Sox 9 and β-catenin was detected by western blotting analysis. Sox 9 has been reported of involvement in transcription of type IX collagen that binds cartilage-specific type II collagen fibrils. β-catenin plays an important role of signaling pathways of cell proliferation although the relationship between β-catenin and mechanical vibration stimulation has not been clarified yet. The obtained results are as follows. The mechanical vibration enhanced the thickness of extracellular matrix of chondrocytes in histologic section at 14 days of culture and increased the expression of Sox 9. In addition, the mechanical vibration significantly increased the number of chondrocytes after 10 days of culture and promoted the expression of β-catenin. These results show that mechanical vibration promotes the matrix production and proliferation of chondrocytes and that a part of important signaling pathways in relation to mechanical vibration stimulation and proliferation of chondrocytes has been revealed.

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