scholarly journals Generation of osteoarthritis and healthy mesenchymal cell lines for research on regenerative medicine for osteoarthritis

2018 ◽  
Vol 26 ◽  
pp. S150 ◽  
Author(s):  
M. Piñeiro-Ramil ◽  
R. Castro-Viñuelas ◽  
C. Sanjuro-Rodríguez ◽  
T. Hermida-Gómez ◽  
I. Fuentes-Boquete ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Cell Lines ◽  
Mesenchymal Cell

scholarly journals Subepithelial fibroblast cell lines from different levels of gut axis display regional characteristics

1998 ◽  
Vol 274 (5) ◽  
pp. G945-G954 ◽  
Author(s):  
Michelina Plateroti ◽  
Deborah C. Rubin ◽  
Isabelle Duluc ◽  
Renu Singh ◽  
Charlotte Foltzer-Jourdainne ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Small Intestine ◽  
Lamina Propria ◽  
Cell Lines ◽  
Transforming Growth Factor ◽  
Mesenchymal Cell ◽  
Proximal Colon ◽  
Proximal Jejunum ◽  
Distal Small Intestine

The intestine is characterized by morphofunctional differences along the proximodistal axis. The aim of this study was to derive mesenchymal cell lines representative of the gut axis. We isolated and cloned rat intestinal subepithelial myofibroblasts raised from 8-day proximal jejunum, distal ileum, and proximal colon lamina propria. Two clonal cell lines from each level of the gut were characterized. They 1) express the specific markers vimentin, smooth muscle α-actin, and smooth muscle myosin heavy chain, revealed by immunofluorescence microscopy and 2) distinctly support endodermal cell growth in a coculture model, depending on their regional origin, and 3) the clones raised from the various proximodistal regions maintain the same pattern of morphogenetic and growth and/or differentiation factor gene expression as in vivo: hepatocyte growth and/or scatter factor and transforming growth factor-β1 mRNAs analyzed by RT-PCR were more abundant, in the colon and ileal clones and mucosal connective tissue, respectively. In addition, epimorphin mRNA studied by Northern blot was also the highest in one ileal clone, in which it was selectively upregulated by all-trans retinoic acid (RA) treatment. Epimorphin expression in isolated 8-day intestinal lamina propria was higher in the distal small intestine and proximal colon than in the proximal small intestine. In conclusion, we isolated and characterized homogeneous cell subtypes that can now be used to approach the molecular regulation of the epithelium-mesenchyme-dependent regional specificity along the gut.

scholarly journals Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4361
Author(s):  
Antonella Sgambato ◽  
Valentina Pastori ◽  
Laura Russo ◽  
Simone Vesentini ◽  
Marzia Lecchi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Nervous System ◽  
Regenerative Medicine ◽  
Neuronal Differentiation ◽  
Cell Lines ◽  
Neuroblastoma Cells ◽  
Biological Significance ◽  
Great Promise ◽  
Cell Behavior ◽  
Carbohydrate Epitopes

The regeneration of the nervous system is a challenging task. Currently, regenerative medicine approaches that exploit nature-inspired cues are being studied and hold great promise. The possibility to use protein-based matrices functionalized with small oligo- and monosaccharides is of interest since these can be finely tuned to better mimic the native environment. Collagen has been selected as a promising material that has the potential to be further tailored to incorporate carbohydrates in order to drive cell behavior towards neuroregeneration. Indeed, the grafting of carbohydrates to collagen 2D matrices is proved to enhance its biological significance. In the present study, collagen 2D matrices were grafted with different carbohydrate epitopes, and their potential to drive F-11 neuroblastoma cells towards neuronal differentiation was evaluated. Collagen functionalized with α-glucosides was able to differentiate neuroblastoma cells into functional neurons, while sialyl α-(2→6)-galactosides stimulated cell proliferation.

BioTime’s bid to end age-related disease: A look at CEO Michael West’s Vision

2015 ◽  
Vol 21 (3) ◽  
Author(s):  
Ian C. Clift
Keyword(s):  
Regenerative Medicine ◽  
Rna Sequencing ◽  
Cell Lines ◽  
Molecular Basis ◽  
Sequencing Technology ◽  
Related Disease ◽  
Age Related ◽  
Pure Cell ◽  
The Common ◽  
Future Growth

The regenerative medicine space is one that is set to explode with considerable innovation and profitability for shrewd biotechnologists. I had the opportunity to speak with Michael West, PhD., CEO of BioTime (BTX) and found a man passionate about regenerative medicine and of course passionate about his role in its future. In this conversation I learned of West's vision, which I think provides some powerful clues as to areas of future growth in the biotech sector. He points to three scientific advances that make this vision actualizable. First, sequencing technology that allows us to perform RNA sequencing for around 300 dollars or less, second the common and reversible molecular basis for age-related diseases, and finally industrial scaling of pure cell lines like the ones manufactured by BTX. Let's look at the three enabling technologies that West touched on and examine how they are being utilized to achieve West's vision within BTX and others involved in the anti-aging revolution.

scholarly journals New epithelial and mesenchymal cell lines from primary liver cancer to study cell interactions in hepatocarcinogenesis

2007 ◽  
Vol 7 (Suppl 2) ◽  
pp. A59
Author(s):  
Sandra Sagmeister ◽  
Maria Eisenbauer ◽  
Christine Pirker ◽  
Klaus Holzmann ◽  
Wolfram Parzefall ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Lines ◽  
Primary Liver Cancer ◽  
Mesenchymal Cell ◽  
Cell Interactions

scholarly journals Variations in 5'-nucleotidase activity in established mesenchymal cell lines from syngeneic A/J mice.

1983 ◽  
Vol 8 (2) ◽  
pp. 119-126
Author(s):  
Ryojin Uchino ◽  
Manabu Fukumoto ◽  
Toshihiro Adachi ◽  
Osamu Midorikawa
Keyword(s):  
Cell Lines ◽  
Mesenchymal Cell ◽  
Nucleotidase Activity

scholarly journals Microarray interrogation of human metanephric mesenchymal cells highlights potentially important molecules in vivo

2007 ◽  
Vol 28 (2) ◽  
pp. 193-202 ◽  
Author(s):  
Karen L. Price ◽  
David A. Long ◽  
Nipurna Jina ◽  
Helen Liapis ◽  
Mike Hubank ◽  
...  
Keyword(s):  
Cell Lines ◽  
Animal Studies ◽  
Kidney Development ◽  
Mesenchymal Cell ◽  
Cellular Processes ◽  
Embryonic Tissues ◽  
Matrix Metalloproteinase 1 ◽  
Vessel Growth ◽  
Normal Human

Many molecules have been implicated in kidney development, often based on experimental animal studies with organ cultures and cell lines. There are very few studies, however, that have directly addressed equivalent living human embryonic tissues. We generated renal mesenchymal cell lines from normal human metanephroi and used a microarray strategy to define changes in gene expression after stimulation with growth factors which enhance nephrogenesis in rodents. Changes were observed in 1) genes modulating diverse general cellular processes, such as matrix metalloproteinase 1 and stanniocalcin 1; 2) genes previously implicated in organogenesis e.g., sprouty 4 and midline 1; and 3) genes involved in blood vessel growth, including angiopoietin 1 and 4. Expression of these same genes was subsequently confirmed in vivo. Our novel data have identified several previously unhighlighted genes that may be implicated in differentiation programs within early human nephrogenesis.

scholarly journals The Use of Stem Cells to Model Amyotrophic Lateral Sclerosis and Frontotemporal Dementia: From Basic Research to Regenerative Medicine

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Erin C. Hedges ◽  
Vera J. Mehler ◽  
Agnes L. Nishimura
Keyword(s):  
Stem Cells ◽  
Amyotrophic Lateral Sclerosis ◽  
Regenerative Medicine ◽  
Frontotemporal Dementia ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Basic Research ◽  
Patient Specific ◽  
Exogenous Dna ◽  
Lateral Sclerosis

In recent years several genes have linked amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) as a spectrum disease; however little is known about what triggers their onset. With the ability to generate patient specific stem cell lines from somatic cells, it is possible to model disease without the need to transfect cells with exogenous DNA. These pluripotent stem cells have opened new avenues for identification of disease phenotypes and their relation to specific molecular pathways. Thus, as never before, compounds with potential applications for regenerative medicine can be specifically tailored in patient derived cultures. In this review, we discuss how patient specific induced pluripotent stem cells (iPSCs) have been used to model ALS and FTD and the most recent drug screening targets for these diseases. We also discuss how an iPSC bank would improve the quality of the available cell lines and how it would increase knowledge about the ALS/FTD disease spectrum.

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