A novel BRCA1 gene deletion detection in human breast carcinoma MCF-7 cells through FRET between quantum dots and silver nanoclusters

The phospholipase D (PLD)-mediated synthesis of phosphatidylethanol (PtdEtOH) and the hydrolysis of phosphatidylethanolamine (PtdEtn) and phosphatidylcholine (PtdCho) were examined in drug-sensitive and multidrug-resistant lines of MCF-7 human breast carcinoma cells. In drug-sensitive (MCF-7/WT) cells, the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) failed to enhance either the synthesis of PtdEtOH or the hydrolysis of either phospholipid. In the drug-resistant (MCF-7/MDR) cells, 100 nM PMA greatly enhanced both the synthesis of PtdEtOH (approximately 21-fold) and the hydrolysis of PtdEtn (approximately 29-fold), but had no effect on the hydrolysis of PtdCho. The PLD activators sphingosine and H2O2 were found to elicit only a slight (1.28-1.4-fold) stimulatory effect on PtdCho hydrolysis in both the MCF-7/WT and MCF-7/MDR cell types, and had only a small effect on PtdEtn hydrolysis in the MCF-7/WT cells as well. However, these agents significantly (approximately 2.6-3.5-fold) stimulated PtdEtn hydrolysis in the MCF-7/MDR cells. These data indicate that MCF-7/MDR cells contain a PtdEtn-specific PLD activity which can be selectively stimulated by PMA, sphingosine and H2O2.

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Salinomycin exerts anticancer effects on human breast carcinoma MCF-7 cancer stem cells via modulation of Hedgehog signaling

Chemico-Biological Interactions ◽

10.1016/j.cbi.2014.12.002 ◽

2015 ◽

Vol 228 ◽

pp. 100-107 ◽

Cited By ~ 35

Author(s):

Ying Lu ◽

Wei Ma ◽

Jun Mao ◽

Xiaotang Yu ◽

Zhenhuan Hou ◽

...

Keyword(s):

Stem Cells ◽

Breast Carcinoma ◽

Cancer Stem Cells ◽

Hedgehog Signaling ◽

Human Breast Carcinoma ◽

Human Breast ◽

Anticancer Effects ◽

Mcf 7

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beta-Carotene is Accumulated, Metabolized, and Possibly Converted to Retinol in Human Breast Carcinoma Cells (MCF-7)

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.74.3.171 ◽

2004 ◽

Vol 74 (3) ◽

pp. 171-177 ◽

Cited By ~ 2

Author(s):

Torres ◽

Borojevic ◽

Trugo

Keyword(s):

Breast Carcinoma ◽

Beta Carotene ◽

Human Breast Carcinoma ◽

Breast Carcinoma Cell Line ◽

Human Breast ◽

Cell Extracts ◽

Human Breast Carcinoma Cells ◽

Dose Dependent ◽

Mcf 7 ◽

In Cells

The aims of the present study were to investigate the uptake, accumulation, and metabolism of beta-carotene by the human breast carcinoma cell line MCF-7. Beta-carotene uptake was time- and dose-dependent, and independent of cell polarity. Beta-carotene accumulation in cells was linear as a function of its concentration in medium (1.3–4.1 mumol/L). It was accompanied by increasing amounts of retinol, which accumulated in cells following a sigmoid pattern, and by other four putative metabolites. Beta-apocarotenals, epoxides, endoperoxides, retinal, retinoic acid, and retinyl esters were not detected in cell extracts. Beta-carotene and its metabolites did not induce alterations in cell morphology or subcellular localization of epithelial mucins. Beta-carotene and retinol were released from cells that had previously accumulated beta-carotene, and were further incubated in beta-carotene- and retinol-free medium, but intracellular retinol content remained constant whereas b-carotene decreased. In conclusion, beta-carotene added to culture medium in physiological concentrations (1–6 mumol/L) is taken up and metabolized in MCF-7 cells, and is possibly converted to retinol.

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