Changes of milk fatty acid composition in four lipid classes as biomarkers for the diagnosis of bovine ketosis using bioanalytical Thin Layer Chromatography and Gas Chromatographic techniques (TLC-GC)

Total cardiac lipids, phospholipids, and the gas-chromatographic pattern of the fatty acids of the various phospholipid fractions separated on thin-layer chromatography were studied before the development of the aorta-ligature cardiac necroses in the rat. The most significant changes were found in the cardiolipin fraction of the treated animals. In this fraction, 24 hours after the ligature of the aorta, there was a relative decrease of C18:2 and increase of two components tentatively identified as long-chain unsaturated fatty acids. Changes in the fatty acid composition of other phospholipids were not considered significant.

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Isolation and Identification of Egg Yolk Cholesteryl Esters

Canadian Journal of Biochemistry ◽

10.1139/o72-183 ◽

1972 ◽

Vol 50 (12) ◽

pp. 1414-1416 ◽

Cited By ~ 5

Author(s):

N. H. Tattrie

Keyword(s):

Fatty Acid Composition ◽

Liquid Chromatography ◽

Fatty Acid ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Egg Yolk ◽

Gas Liquid Chromatography ◽

Cholesteryl Esters ◽

Isolation And Identification ◽

Layer Chromatography

Cholesteryl esters and cholesterol were isolated from egg yolk lipids by thin-layer chromatography. The cholesteryl esters and cholesterol contents of egg yolk lipids were 0.34% and 3.64%, respectively, as determined by gas–liquid chromatography. The fatty acid composition of the cholesteryl esters was 16:0, 24.7%; 16:1, 4.6%; 18:0, 6.8%; 18:1, 53.3%; and 18:2, 10.7%.

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Identification of Plasma Fatty Acids in Four Lipid Classes to Understand Energy Metabolism at Different Levels of Ketonemia in Dairy Cows Using Thin Layer Chromatography and Gas Chromatographic Techniques (TLC-GC)

Animals ◽

10.3390/ani10040571 ◽

2020 ◽

Vol 10 (4) ◽

pp. 571 ◽

Cited By ~ 1

Author(s):

Enrico Fiore ◽

Rossella Tessari ◽

Massimo Morgante ◽

Matteo Gianesella ◽

Tamara Badon ◽

...

Keyword(s):

Fatty Acids ◽

Thin Layer ◽

Dairy Cows ◽

Thin Layer Chromatography ◽

Metabolic Diseases ◽

Lipid Classes ◽

Plasma Fatty Acids ◽

Chromatographic Techniques ◽

Holstein Friesian ◽

Layer Chromatography

Excessive mobilization of adipose tissue in high milk producing dairy cows predisposes to metabolic diseases. The aim of this research was to identify the plasma fatty acids in four lipid classes as biomarkers for the diagnosis of hyperketonemia in bovines using thin layer chromatography and gas chromatographic techniques (TLC-GC). Sixty multiparous Holstein–Friesian dairy cows were enrolled in the study. Blood samples from the coccygeal vein were collected and β-hydroxybutyrate (BHB) was evaluated. All animals were divided into three groups on the basis of ketonemia: BHB < 0.50 mmol/L, 0.50 < BHB < 1.0 mmol/L, and BHB > 1.0 mmol/L. Plasma fatty acid concentrations were evaluated in four lipid classes: Free Fatty Acids (FFA), Triglycerides (TG), Cholesterol Esters (CE) And Phospholipids (PL). The concentration of fatty acids was analyzed using TLC-GC. The results showed the following significance in the lipid classes: 19 fatty acids were significant (p < 0.053) in FFA, nine fatty acids were significant (p < 0.050) in TG, eight fatty acids were significant (p < 0.050) in CE and three fatty acids were significant (p < 0.049) in PL. Eleven parameters were considered as predictive fatty acids related to animals in hyperketonemia. The FFA increased simultaneously with blood BHB levels, although the identified predictive fatty acids related to the TG and CE lipid classes decreased, meanwhile the BHB values increased. In the PL lipid class, no fatty acids were predictive.

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