ABSTRACTBacterial virulence factor production is a highly coordinated process. The temporal pattern of bacterial gene expression varies in different host anatomic sites to overcome niche-specific challenges. The human pathogen group A streptococcus (GAS) produces a potent secreted protease, SpeB, that is crucial for pathogenesis. Recently, we discovered that a quorum sensing pathway comprised of a leaderless short peptide, SpeB-inducing peptide (SIP), and a cytosolic global regulator, RopB, controlsspeBexpression in concert with bacterial population density. The SIP signaling pathway is activein vivoand contributes significantly to GAS invasive infections. In the current study, we investigated the role of the SIP signaling pathway in GAS-host interactions during oropharyngeal colonization. The SIP signaling pathway is functional during growthex vivoin human saliva. SIP-mediatedspeBexpression plays a crucial role in GAS colonization of the mouse oropharynx. GAS employs a distinct pattern of SpeB production during growthex vivoin saliva that includes a transient burst ofspeBexpression during early stages of growth coupled with sustained levels of secreted SpeB protein. SpeB production aids GAS survival by degrading LL37, an abundant human antimicrobial peptide. We found that SIP signaling occurs during growth in human bloodex vivo. Moreover, the SIP signaling pathway is critical for GAS survival in blood. SIP-dependentspeBregulation is functional in strains of diverseemmtypes, indicating that SIP signaling is a conserved virulence regulatory mechanism. Our discoveries have implications for future translational studies.