Medical ozone therapy reduces oxidative stress and intestinal damage in an experimental model of necrotizing enterocolitis in neonatal rats

Purpose: To determine the efficacy of resveratrol in mitigating necrotizing enterocolitis (NEC) in neonatal rats.Methods: Necrotizing enterocolitis (NEC) was induced in neonatal rats using hypoxia and hypothermia. At the completion of treatment, the intestinal tissues of the rats were isolated for evaluation of various biochemical parameters.Results: There was significant increase in the levels of proinflammatory cytokines (TNF-α, IL-6 and IL-1β) and oxidative stress markers (MDA, xanthine oxidase and nitric oxide) in intestinal tissues of NEC rats (p < 0.05). However, resveratrol treatment led to significant decrease in the levels of cytokines and oxidative stress parameters, relative to the NEC group (p < 0.05). Furthermore, Western blotting resultsshowed up-regulation in protein expressions of inflammatory cytokines in NEC rats. However, the protein expressions of inflammatory cytokines were down-regulated in the NEC rats on treatment with resveratrol. Moreover, resveratrol reversed the NEC-induced up-regulations of Bax and caspase-3, as well as NEC-mediated down-regulation of Bcl-2.Conclusion: These results demonstrate that resveratrol mitigates NEC-induced intestinal damage in neonatal rats via anti-inflammatory, anti-apoptotic and antioxidant mechanisms of action. Therefore, resveratrol is a potential therapeutic agent for NEC.

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Medical ozone therapy reduces oxidative stress and testicular damage in an experimental model of testicular torsion in rats

International braz j urol ◽

10.1590/s1677-5538.ibju.2016.0546 ◽

2017 ◽

Vol 43 (6) ◽

pp. 1160-1166 ◽

Cited By ~ 6

Author(s):

Mustafa Tusat ◽

Ahmet Mentese ◽

Selim Demir ◽

Ahmet Alver ◽

Mustafa Imamoglu

Keyword(s):

Oxidative Stress ◽

Experimental Model ◽

Testicular Torsion ◽

Testicular Damage ◽

Ozone Therapy ◽

Medical Ozone

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Protective effects of colchicine in an experimental model of necrotizing enterocolitis in neonatal rats

Journal of Surgical Research ◽

10.1016/j.jss.2013.01.002 ◽

2013 ◽

Vol 183 (1) ◽

pp. 156-162 ◽

Cited By ~ 10

Author(s):

Sadik Yurttutan ◽

Ramazan Ozdemir ◽

Fuat Emre Canpolat ◽

Mehmet Yekta Oncel ◽

Bulent Uysal ◽

...

Keyword(s):

Experimental Model ◽

Necrotizing Enterocolitis ◽

Protective Effects ◽

Neonatal Rats

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Effects of oxidative stress induced by perinatal asphyxia on catalase activity in hippocampus of neonatal rats.

10.26226/morressier.5785edc8d462b80296c996e6 ◽

2016 ◽

Author(s):

Carolyne Lespay-Rebolledo

Keyword(s):

Oxidative Stress ◽

Catalase Activity ◽

Perinatal Asphyxia ◽

Neonatal Rats

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McN-A-343, a muscarinic agonist, reduces inflammation and oxidative stress in an experimental model of ulcerative colitis

Life Sciences ◽

10.1016/j.lfs.2021.119194 ◽

2021 ◽

Vol 272 ◽

pp. 119194

Author(s):

Diva de Aguiar Magalhães ◽

Jalles Arruda Batista ◽

Stefany Guimarães Sousa ◽

Jayro dos Santos Ferreira ◽

Lauanda da Rocha Rodrigues ◽

...

Keyword(s):

Oxidative Stress ◽

Ulcerative Colitis ◽

Experimental Model ◽

Muscarinic Agonist ◽

And Oxidative Stress

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Medical ozone therapy decreases postoperative uterine adhesion formation in rats

Fertility and Sterility ◽

10.1016/j.fertnstert.2011.07.575 ◽

2011 ◽

Vol 96 (3) ◽

pp. S147

Author(s):

S. Demirbag ◽

B. Uysal ◽

A. Guven ◽

N. Yesildaglar ◽

I. Surer

Keyword(s):

Adhesion Formation ◽

Ozone Therapy ◽

Medical Ozone

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The preventive role of medical ozone therapy on remote organ injury induced by hepatic ischemia reperfusion

Annals of Medical Research ◽

10.5455/annalsmedres.2020.09.965 ◽

2021 ◽

Vol 28 (9) ◽

pp. 1671

Author(s):

Levent Demirtas ◽

Cebrail Gursul ◽

Ahmet Gurbuzel ◽

Ilyas Sayar ◽

Mehmet Gurbuzel ◽

...

Keyword(s):

Ischemia Reperfusion ◽

Organ Injury ◽

Ozone Therapy ◽

Hepatic Ischemia ◽

Remote Organ ◽

Remote Organ Injury ◽

Medical Ozone ◽

Hepatic Ischemia Reperfusion ◽

Preventive Role

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Evaluation of the heme oxygenase-1 expression in esophagitis and esophageal cancer induced by different reflux experimental models and diethylnitrosamine

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502010000300015 ◽

2010 ◽

Vol 25 (3) ◽

pp. 304-310 ◽

Cited By ~ 6

Author(s):

Cleber Rosito Pinto Kruel ◽

Luis Felipe Ribeiro Pinto ◽

Tania Cristina Moita Blanco ◽

Theresa Christina Barja-Fidalgo ◽

Levi Lourenzo Melo ◽

...

Keyword(s):

Oxidative Stress ◽

Experimental Model ◽

Heme Oxygenase ◽

Inflammatory Cells ◽

Acid Reflux ◽

Experimental Models ◽

Control Group ◽

Heme Oxygenase 1 ◽

Duodenal Reflux ◽

Esophageal Carcinogenesis

PURPOSE: To study the expression of heme-oxygenase-1 (HO-1), an enzyme induced by oxidative stress, in specimens obtained from an experimental model in rats that evaluated the role of gastric and duodenal reflux in esophageal carcinogenesis. METHODS: Esophageal specimens embedded in paraffin obtained from different experimental groups of rats were used for immunohistochemistry analysis of HO-1 expression. The rats had been divided into the following groups and were killed after 22 weeks: (1) cardioplasty to induce acid reflux; (2) esophagoduodenal anastomosis to induce duodenal reflux; (3) no treatment; (4) cardioplasty + diethylnitrosamine (DEN); (5) esophagoduodenal anastomosis + DEN; and (6) DEN. The study sample comprised 3 specimens from each group with the most severe histopathological lesions found on each study branch. RESULTS: The expression of HO-1 was seen only in rat specimens submitted to esophagoduodenal anastomosis (Groups 2 and 5), and the analysis of mean fluorescence intensity revealed a significant increase of HO-1 expression (4.8 and 4.6 fold, respectively) when compared with the control group (Group 3) (p<0.05). The main target for HO-1 induction was the inflammatory cells inside the tumor or in subepithelial areas. Rats exposed to gastric reflux had no HO-1 expression. CONCLUSION: Reflux esophagitis induced by reflux of duodenal contents, which provoked considerable oxidative stress, may play an important role in esophageal carcinogenesis. Acid reflux did not induce oxidative stress in this experimental model.

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