Antibacterial and antioxidant potential of biosynthesized copper nanoparticles mediated through Cissus arnotiana plant extract

Synthesis of nanoparticles of transition metals by using medicinal plants has been outstreched in recent years because of the characteristic features which are embodied in the end product. This work is proceded with the aim to synthesize and optimize copper nanoparticles (CuNPs-Pl) using aqueous extract of Polyalthia longifolia leaves (PlL) for characterization and evaluation of antimicrobial and antioxidant potential. The synthesis of CuNPs-Pl was confirmed by visual inspection of the dark brown residues in the reaction flask and via absorption band around 580nm by UV/Visible spectroscopy. Synthesis process was optimized through investigation of environmental variables. FTIR analysis was carried out for both PIL and CuNPs-Pl which identified the presence of alkanes, alcoholic, and aldehydic groups in the PlL and their encapsulation on the copper surface. The synthesized CuNPs-Pl were found to be spherical and rod shaped, and polydispersed when investigated through SEM study. Similarly, these nanoparticles had monoclinic structure and crystalline nature when analyzed by XRD. Moreover, these nanoparticles showed metallic form when EDX examination was done. Further, biological activities were performed. The synthesized nanoparticles showed considerable inhibition zones against Escherichia coli (12mm), Bacillus subtilis (11mm), Aspergillus niger (10mm), and Schyzophyllum commune (16mm) which depicted their powerful antibacterial and antifungal activity. Likewise, CuNPs-Pl were effeciently able to quench free radicals as indicated from the 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), and nitric oxide (NO) assays by exhibiting 86.32, 50.45, and 48.23% inhibition respectively. Thus, the contemporary work has substantiated that low cost CuNPs-Pl can be highly proficient alternate or substitute of synthetic formulations.

Download Full-text

Preparation of herbal formulation and it’s application on nanoparticles synthesis and antibacterial activity

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10i3.1447 ◽

2019 ◽

Vol 10 (3) ◽

Author(s):

Srijan Sunar ◽

Rajeshkumar S ◽

Anitha Roy ◽

Lakshmi T

Keyword(s):

Antibacterial Activity ◽

Copper Nanoparticles ◽

Plant Extract ◽

Chemical Reduction ◽

Diffusion Method ◽

Vis Spectroscopy ◽

Double Beam ◽

Dark Green ◽

Anti Fungal ◽

Important Progress

Copper nanoparticles makes important progress in the area of nanotechnology and nanomedicine due to their good optical, electrical and anti-fungal/bacterial application. It is prepared using some methods such as vacuum vapour deposition, microwave irradiation methods, chemical reduction and laser ablation. The chemical reduction method is simple, inexpensive and gives a liable control of geometrical nanoparticle characteristics like size and shape. 20 millimolar of 80 ml copper sulphate prepared using double distilled water. The plant extract is added with the metal solution and was made into 100 ml solution. The synthesised nanoparticles solution is preliminarily characterized by using UV- vis-spectroscopy, 3ml of the solution is taken in curette and scanned in double beam UV-vis- spectrophotometer from 300 nm to 700 nm wavelength. The agar well diffusion method is used. Different concentration of Cu NPs was tested against Staphylococcus aureus, Streptococcus mutans (gram +), Enterococcus sp and Pseudomonas sp. The result reveals that Moringa Oleifera mediated with copper nanoparticles show effective antibacterial activity. CuNPs ex significantly higher activity with an increase in the zone of inhibition diameter. The plant extract is observed to be dark green, and the copper nanoparticles are seen to be in light greenish in colour. They can be used in toothpaste and oral medicines due to their antibacterial activity. Nanoparticles are expected to be used in future for the effective drug systems and immunity against diseases.

Download Full-text

Biosynthesis of copper nanoparticles from plant extract and bacteria - a review

International Journal of Nanoparticles ◽

10.1504/ijnp.2021.118104 ◽

2021 ◽

Vol 13 (3) ◽

pp. 135

Author(s):

Ravindra Ashok Sharma

Keyword(s):

Copper Nanoparticles ◽

Plant Extract

Download Full-text

In-vitro antioxidant, antidiabetic and toxic effect of Ageratum houstonianum from Chitwan district Nepal

Journal of Balkumari College ◽

10.3126/jbkc.v9i1.30067 ◽

2020 ◽

Vol 9 (1) ◽

pp. 48-54

Author(s):

Khaga Raj Sharma

Keyword(s):

Toxic Effect ◽

Enzyme Inhibition ◽

Plant Extract ◽

Methanolic Extract ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Moderate Activity ◽

Dependent Manner ◽

Phytotoxic Activity

Medicinal plants are safe and the oldest natural products used for many years to conserve food, to treat health disorders and to prevent diseases. The active chemical compounds formed during secondary vegetal metabolism is usually responsible for the biological properties of some plant species used throughout the world for various purposes including treatment of diabetes, cancer, infectious diseases etc. The present study was undertaken to analyze the phytochemicals by colour differentiation method, to evaluate the toxic effect by phytotoxic assay, antidiabetic activity by α amylase enzyme inhibition and antioxidant potential by DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity of methanolic extract of Ageratum houstonianum. Treatment of problem in carbohydrate uptake needed the inhibition of α-amylase plays a role in the digestion of polysaccharide and glycogen, is made a strategy for controlling diabetes. For this study whole plant was collected, dried and the powder was made. The extraction was carried out by cold percolation in which methanol was used as a solvent. The methanolic extract was subjected to In-vitro phytotoxic activity by adopting the standard protocol. The α-amylase enzyme inhibition activity of plant extract was carried out by using starch as substrate, pancreatic α amylase as the enzyme, and acarbose as standard. The result of in-vitro phytotoxic bioassay revealed that the plant extract showed moderate activity with percentage growth regulation 80 and 25 percent in a concentration-dependent manner. The α-amylase enzyme inhibition was 74.13 to 99.39 percent in a dose-dependent manner. The antioxidant potential of Ageratum houstonianum extract showed mild activity with IC50 123.67 μg/ml as compared to the standard ascorbic acid IC50 5.38 μg/ ml. It is concluded from the present study that Ageratum houstonianum could be used as a natural source to isolate antioxidant, anti-hyperglycemic agent, herbicide and weedicide as it shows a good α amylase inhibition, radical scavenging and phytotoxic activity respectively.

Download Full-text

Some Biological activities of ethanol extract of Marrubium globosum

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9i6.1129-1132.4382 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1129-1132

Author(s):

Mustafa Pehlivan ◽

Falah Saleh Mohammed ◽

Ali Erdem Şabik ◽

Eylem Kına ◽

Muhittin Dogan ◽

...

Keyword(s):

Plant Extract ◽

Polar Solvent ◽

Biological Activities ◽

Ethanol Extract ◽

Antibacterial Activities ◽

Natural Antioxidant ◽

Antioxidant Potential ◽

Dilution Method ◽

Agar Dilution Method ◽

Agar Dilution

Plants have been used for centuries to treat various diseases. In this study, Marrubium globosum Montbret & Aucher ex Benth. plant was used as a material. The extract of the plant was extracted with the help of soxhlet device using ethanol, which is a good polar solvent, and Rel Assay kits were used to determine the oxidant and antioxidant levels in the plant extract. Antifungal and antibacterial activities of M. globossum were tested against standard bacteria and fungus strains by agar dilution method. As a result of the analysis, TAS value of plant extract was determined as 7.677±0.231, TOS value as 12.387±0.083 and OSI value as 0.162±0.004. In this context, it has been observed that the plant has an important antioxidant potential. In addition, the plant extract was found to be effective against test microorganisms at 50-200 μg/mL extract concentrations. As a result, it has been determined that M. globossum can be a natural antioxidant and antimicrobial source.

Download Full-text

Synthesis and Catalytic Applications of Ultrasmall Copper Nanoparticles Using Aloe Vera Plant Extract

10.26434/chemrxiv.13187780 ◽

2020 ◽

Author(s):

Rajagopal Ramkumar ◽

Shradha Sapru ◽

Panthalattu Parambil Archana

Keyword(s):

Catalytic Activity ◽

Copper Nanoparticles ◽

Plant Extract ◽

Aloe Vera ◽

Catalyst Loading ◽

Water Medium ◽

Reaction Conditions ◽

Three Component Reaction ◽

Catalytic Applications ◽

Low Catalyst Loading

<div>An extract of <i>Aloe Vera</i> acts as a powerful reducing agent for the synthesis of ultrasmall copper nanoparticles (CuNPs) in water medium. The prepared copper nanoparticles are characterized by XRD, HRTEM and UV-Vis analysis. The diameter of the prepared nanoparticle is less than 5 nm. The catalytic activity of CuNPs has been successfully evaluated for the three-component reaction to synthesize 1,2,3-triazoles and chalcone tethered 1,2,3-triazoles with low catalyst loading and under mild reaction conditions. These triazoles are further utilized for the synthesis of dihydropyrazines.</div>

Download Full-text

ESTIMATION OF PHENOLIC CONTENT, FLAVONOID CONTENT, ANTIOXIDANT, AND ALPHA-AMYLASE INHIBITORY ACTIVITY OF SOME SELECTED PLANTS FROM SIRAHA DISTRICT NEPAL

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i4.36734 ◽

2020 ◽

pp. 18-23

Author(s):

Numer Alam ◽

Khaga raj Sharma

Keyword(s):

Plant Extract ◽

Plant Extracts ◽

Inhibitory Activity ◽

Brine Shrimp ◽

Phenolic Content ◽

Antioxidant Potential ◽

Tinospora Cordifolia ◽

Total Phenolic ◽

Phytochemical Screening ◽

Flavonoid Content

Objectives: The purpose of this research was to estimate the phenolic content, flavonoid content, antioxidant, antibacterial, and α-amylase inhibitory activity of some selected plants such as Anethum sowa, Trigonella foenum-graecum, Lepidium sativum, Cuscuta reflexa, Eclipta alba, Leucas cephalotes, and Tinospora cordifolia collected from Siraha district of Nepal using in vitro studies. Methods: Methanol extracts of these medicinal plants were prepared by cold percolation method. Preliminary phytochemical screening was performed by color differentiation method. Total phenolic and flavonoid content were estimated by Folin-Ciocalteu reagent method and aluminum chloride colorimetric method. Antioxidant potential was evaluated by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Furthermore, the α-amylase enzyme inhibitory activity was studied using starch as a substrate, pancreatic α-amylase as the enzyme, and acarbose as standard. Results: Phytochemical screening showed that the plant extracts were found a rich source of secondary metabolites. The phenolic content estimation showed T. foenum-graecum 939.764±0.01, L. sativum 551.63±0.02, A. sowa 306.34±0.06, L. cephalotes 233.19±0.03, T. cordifolia 211.76±0.02, E. alba 202.67±0.02, and C. reflexa Roxb. 145.09±0.09 mg milligram gallic acid equivalent per gram. The flavonoid content estimation showed T. cordifolia 852.07±0.11, L. sativum 553.81±0.05, E. alba 322.13±0.02, A. sowa 329.02±0.05, L. cephalotes 164.93±0.02, and C. reflexa Roxb. 146.37±0.00 mg milligram quercetin equivalent per gram. The antioxidant potential showed by E. alba IC50 33.48±0.82 μg/ml and the values ranged from E. alba 33.48±0.82 to A. sowa 47.62±1.09 μg/ml. The α-amylase inhibitory activity showed by A. sowa 76.78±2.00–E. alba 777.36±9.66 μg/ml. The result of brine shrimp toxicity showed LC50 value >1000 μg/ml. Among the seven plant extracts, only the plant extract of E. alba showed a zone of inhibition 14 mm against Staphylococcus aureus. Conclusions: The plant extract of T. foenum-graecum showed the highest phenolic content, and T. cordifolia showed the highest flavonoid content. The highest antioxidant potential exhibited by E. alba and the highest α-amylase inhibition activity showed by A. sowa. The plant extract of E. alba showed moderate antibacterial activity against S. aureus. All plant extracts were found non-toxic against brine shrimp larvae although further study is needed to assess its mechanism of action.

Download Full-text