scholarly journals Some Biological activities of ethanol extract of Marrubium globosum

2021 ◽  
Vol 9 (6) ◽  
pp. 1129-1132
Author(s):  
Mustafa Pehlivan ◽  
Falah Saleh Mohammed ◽  
Ali Erdem Şabik ◽  
Eylem Kına ◽  
Muhittin Dogan ◽  
...  
Keyword(s):  
Plant Extract ◽  
Polar Solvent ◽  
Biological Activities ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Natural Antioxidant ◽  
Antioxidant Potential ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution

Plants have been used for centuries to treat various diseases. In this study, Marrubium globosum Montbret & Aucher ex Benth. plant was used as a material. The extract of the plant was extracted with the help of soxhlet device using ethanol, which is a good polar solvent, and Rel Assay kits were used to determine the oxidant and antioxidant levels in the plant extract. Antifungal and antibacterial activities of M. globossum were tested against standard bacteria and fungus strains by agar dilution method. As a result of the analysis, TAS value of plant extract was determined as 7.677±0.231, TOS value as 12.387±0.083 and OSI value as 0.162±0.004. In this context, it has been observed that the plant has an important antioxidant potential. In addition, the plant extract was found to be effective against test microorganisms at 50-200 μg/mL extract concentrations. As a result, it has been determined that M. globossum can be a natural antioxidant and antimicrobial source.

scholarly journals Antifungal and Antibacterial Metabolites from a French Poplar Type Propolis

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Séverine Boisard ◽  
Anne-Marie Le Ray ◽  
Anne Landreau ◽  
Marie Kempf ◽  
Viviane Cassisa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Gram Positive ◽  
Weak Activity ◽  
Agar Dilution

During this study, thein vitroantifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a French propolis batch were evaluated. Antifungal activity was evaluated by broth microdilution on three pathogenic strains:Candida albicans, C. glabrata, andAspergillus fumigatus. Antibacterial activity was assayed using agar dilution method on 36 Gram-negative and Gram-positive strains includingStaphylococcus aureus. Organic extracts showed a significant antifungal activity againstC. albicansandC. glabrata(MIC80between 16 and 31 µg/mL) but only a weak activity towardsA. fumigatus(MIC80= 250 µg/mL). DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially againstS. aureus(SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC10030–97 µg/mL). A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

scholarly journals Anti-HIV, Antitubercular and Antibacterial Activities of Novel 3-(Substituted Quinazolinylamino)-2-phenyl quinazolin-4(3H)ones

2019 ◽  
Vol 32 (2) ◽  
pp. 281-286
Author(s):  
M.T. Sulthana ◽  
K. Chitra ◽  
V. Alagarsamy
Keyword(s):  
Antibacterial Activity ◽  
Antitubercular Activity ◽  
Antibacterial Activities ◽  
Primary Amines ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
E Coli ◽  
Agar Dilution ◽  
Anti Hiv Agents ◽  
Anti Hiv

In the present study, we have synthesized a series of novel 2-phenyl-3-(substituted quinazolinylamino)quinazolin-4(3H)-ones by the reaction of 3-(substituted)-2-hydrazinoquinazoline-4(3H)-ones with 2-phenyl-3,1-benzoxazin-4-one. The starting material 3-(substituted)-2-hydrazinoquinazolin-4(3H)-ones were synthesized from various primary amines. All the synthesized compounds were screened for their antitubercular, anti-HIV and antibacterial activity against different Grampositive and Gram-negative strains by agar dilution method. Among the test compounds, 3-(4-nitrophenyl)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one (BQZ6) and 3-(4-chlorophenyl)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one (BQZ7) shown most potent antibacterial activity against E. coli, P. aeruginosa and S. aureus with the MIC of 3 μg/mL. The compound BQZ7 exhibited the antitubercular activity with the MIC of 25 μg/mL and anti-HIV activity with the MIC of 35.4 μg/mL against HIV1 and HIV2 and offers potential lead for further optimization and development to new antitubercular and anti-HIV agents. The results from this study confirm that the synthesized and biologically evaluated quinazolines showed promising antimicrobial, antitubercular and anti-HIV activities and are new scaffolds for antimicrobial activity

scholarly journals Synthesis andIn VitroAntibacterial Activity of New 2-(1-Methyl-4-nitro-1H-imidazol-5-ylsulfonyl)-1,3,4-thiadiazoles

2011 ◽  
Vol 8 (3) ◽  
pp. 1120-1123 ◽  
Author(s):  
Bahram Letafat ◽  
Negar Mohammadhosseini ◽  
Ali Asadipour ◽  
Alireza Foroumadi
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Agar Dilution

In the present study we report the synthesis and antibacterial activity of a new series 2-(1-methyl-4-nitro-1H-imidazol-5-ylsulfonyl)-1,3,4-thiadiazoles (6a-c). Compounds6a-cwere testedin vitroby the conventional agar dilution method against a panel of microorganisms including gram-negative and gram-positive bacteria. Compound6bwith 5-(5-nitrofuran-2-yl)-residue on 1,3,4-thiadiazole scaffold have shown promising antibacterial activities against gram-positive bacteria includingStaphylococcus aureus, Staphylococcus epidermidisandBacillus subtilis.

scholarly journals Datura stramonium (Solanaceae): Antioxidant and Antimicrobial Potentials

2021 ◽  
Vol 9 (4) ◽  
pp. 818-821
Author(s):  
Falah Saleh Mohammed ◽  
Eylem Kına ◽  
Mustafa Sevindik ◽  
Muhittin Dogan ◽  
Mustafa Pehlivan
Keyword(s):  
Alternative Medicine ◽  
Plant Extract ◽  
Datura Stramonium ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Treatment And Prevention ◽  
Agar Dilution ◽  
Materials Used ◽  
Ethanol Extracts ◽  
Different Parts

Many people in different parts of the world benefit from alternative medicine in the treatment and prevention of diseases. Plants are among the important natural materials used in alternative medicine. In this study, the antioxidant and antimicrobial potential of Datura stramonium L. was determined. Ethanol extracts of the plant's flower parts were obtained in the soxhlet device. The antioxidant and oxidant potential of the plant extract was determined using Rel Assay TAS and TOS kits. Antimicrobial activity was tested by the agar dilution method. The TAS value of the plant extract was 7.559±0.224 mmol/L, the TOS value was 10.711±0.243 μmol/L, and the OSI value was 0.142±0.002. It was determined that the plant extract was effective against bacteria and fungus strains at 100-400 μg/mL concentration. As a result, it was determined in our study that D. stramonium can be a natural antioxidant and antimicrobial source.

scholarly journals Effects of selected Taiwanese endemic plants on anti-lipid peroxidation and antibacterial activities against clinically isolated extended-spectrum-β-lactamase producing Klebsiella pneumoniae and Escherichia coli

2021 ◽  
Author(s):  
Po-Wei Tsai ◽  
Pei-Chin Lin ◽  
Ling-Ling Yang ◽  
Ming Shun Wu
Keyword(s):  
Escherichia Coli ◽  
Lipid Peroxidation ◽  
Medicinal Plants ◽  
Antibacterial Activities ◽  
Inhibitory Effect ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Kidney Mitochondria ◽  
E Coli ◽  
Agar Dilution

Abstract It has been a challenge for many clinicians to treat a complicated extended-spectrum-β-lactamase (ESBL) producing Klebsiella Pneumoniae (Kp) and Escherichia coli (E. coli) infection due to widespread antibiotic abuse with renal damage as one of its common side effects. Therefore, this study aimed to assess the antibacterial activity of extracts from several Taiwanese folk medicinal plants against ESBL- Kp and E. coli. with renal protecting ability against lipid peroxidation (LPO) on mice kidney mitochondria. Preliminary antibacterial activities of ethanol extracts from twenty (20) Taiwanese folk medicinal plants were measured by agar-dilution method against standard ESBL strains of E. coli (ATCC 25922, ATCC 35218) and Kp (ATCC 23856, ATCC 700603). Rhus semialata var. roxburghiana DC. (RSR) exerted the most inhibitory effect and then further extracted with n-hexane, ethyl acetate, acetone, ethanol, and water, respectively. Each extract also evaluated against the four standard ATCC microorganisms. Their MIC50, MIC90, and time kill assay were adapted with detecting the maximum inhibitory activities and the antibacterial spectrum range of each extract was measured against twenty-four (24) kinds of microbes. Which were used including gram-positive, gram-negative bacteria and fungus by agar dilution method. Finally, renal protective ability was detected inhibitory effect of ferrous induced lipid peroxidation on mice mitochondria. Among 20 Taiwanese folk medicinal plants tested, Rhus semialata var. roxburghiana DC. (RSR) exhibited maximum inhibition against clinical ESBL-producing Kp and E. coli strains with acetone extracts showing MIC50/MIC90 values at 1000 µg/mL, the course of antimicrobial action was bacteriostatic and with inhibitions to all 24 kinds of microbial including Gram positive and negative bacteria and fungi. Furthermore, result of thiobarbituric acid reactive substances (TBARS) assay from this extract showed high lipid peroxidative (LPO) protective capability on mice kidney mitochondria (IC50: 29.29 ± 0.35µg/mL). RSR acetone extract, with its maximum activity against clinical isolated ESBL-producing Kp and E. coli, antimicrobial effect against other wide spectral range bacteria and relatively high LPO protective ability on mice kidney mitochondria, is a potential source, albeit further studies have yet to be conducted, to develop an antimicrobial drug against ESBL-Kp and E. coli.

Antibacterial Activity of Silver-Hydroxyapatite/Titania Nanoparticles on Oral Bacteria

2007 ◽  
Vol 330-332 ◽  
pp. 299-302 ◽  
Author(s):  
Juan Liao ◽  
An Chun Mo ◽  
Hong Kun Wu ◽  
Jing Chao Zhang ◽  
Yu Bao Li ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Agar Plate ◽  
Antibacterial Activities ◽  
Oral Bacteria ◽  
Titania Nanoparticles ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Bacterial Strains ◽  
Bacterial Accumulation ◽  
Agar Dilution

Since bacterial accumulation surrounding biomaterials has pathogens known to cause the biomaterials centered infection, it may be important to develop some kind of biomaterial with antibacterial activity as well as biocompatibility. This study focused on evaluating the antibacterial activity of silver-hydroxyapatite/ Titania nanoparticles (Ag-nHA/nTiO2) against oral bacteria with agar dilution method. Bacteria were seeded on agar plate containing antibacterial material with different concentrations after incubation of 48 hours. The antibacterial activity was demonstrated by MICs. The MICs of Ag-nHA/nTiO2 ranged between 1000μg/ ml and 7500μg /ml under anaerobic conditions. And it also exhibited remarkable antibacterial activity to all the aerobe bacteria (MIC≤500μg/ml). On the other hand, the antibacterial activities of Ag-nHA/nTiO2 differentiate to some extent with the bacterial strains. This Ag-nHA/nTiO2 exhibited remarkable antibacterial activity to anaerobic and aerobe bacteria. This antibacterial effect may reduce the potential for bacterial colonisation of oral biomaterials with Ag-nHA/nTiO2.

scholarly journals Evaluation of the Bioactivities of Rumex crispus L. Leaves and Root Extracts Using Toxicity, Antimicrobial, and Antiparasitic Assays

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Oladayo Amed Idris ◽  
Olubunmi Abosede Wintola ◽  
Anthony Jide Afolayan
Keyword(s):  
Brine Shrimp ◽  
Ethanol Extract ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Rumex Crispus ◽  
Root Extracts ◽  
Brine Shrimp Lethality Assay ◽  
Microbial Infections ◽  
Agar Dilution ◽  
Antimicrobial Potency

Traditional folks in different parts of the world use Rumex crispus L. for the treatment of microbial infections, malaria, and sleeping sickness in the form of decoction or tincture. In the search for a natural alternative remedy, this study aimed to evaluate the antimicrobial, antitrypanosomal, and antiplasmodial efficacy and the toxicity of R. crispus extracts. Antimicrobial potency of the extracts was evaluated using the agar dilution method to determine the minimum inhibitory concentration (MIC). The antitrypanosomal activity of the extracts was evaluated with the Trypanosoma brucei brucei model while the antimalaria potency was tested using Plasmodium falciparum 3D7 strain. Toxicity was then tested with brine shrimp assay and cytotoxicity (HeLa cells). The acetone extract of the root (RT-ACE) reveals the highest antimicrobial potency with the lowest MIC value of <1.562 mg/mL for all bacteria strains and also showed high potent against fungi. RT-ACE (IC50: 13 μg/mL) and methanol extract of the leaf (LF-MEE; IC50: 15 μg/mL) show a strong inhibition of P. falciparum. The ethanol extract of the root (RT-ETE: IC50: 9.7 μg/mL) reveals the highest inhibition of T.b. brucei parasite. RT-ETE and RT-ACE were found to have the highest toxicity in brine shrimp lethality assay (BSLA) and cytotoxicity which correlates in the two assays. This research revealed Rumex crispus has potency against microorganisms, Trypanosoma, and Plasmodium and could be a potential source for the treatment of these diseases.

scholarly journals Evaluation of the Antimicrobial Activity of Some Components of the Essential Oils of Plants Used in the Traditional Medicine of the Tehuacán-Cuicatlán Valley, Puebla, México

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 295
Author(s):  
Sebastián Candelaria-Dueñas ◽  
Rocío Serrano-Parrales ◽  
Marisol Ávila-Romero ◽  
Samuel Meraz-Martínez ◽  
Julieta Orozco-Martínez ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Qualitative Evaluation ◽  
Bactericidal Effect ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Diffusion Technique ◽  
Agar Dilution

In Tehuacán-Cuicatlán valley (Mexico), studies have been carried out on the essential oils of medicinal plants with antimicrobial activity and it was found that they present compounds in common such as: α-pinene, β-pinene, carvacrol, eugenol, limonene, myrcene, ocimene, cineole, methyl salicylate, farnesene, and thymol. The goal of this study was to assess the antimicrobial activity of essential oils’ compounds. The qualitative evaluation was carried out by the Kirby Baüer agar diffusion technique in Gram-positive bacteria (11 strains), Gram-negative bacteria (18 strains), and yeasts (8 strains). For the determination of the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), the agar dilution method was used. All the evaluated compounds presented antimicrobial activity. The compounds eugenol and carvacrol showed the largest inhibition zones. Regarding yeasts, the compounds ocimene, cineole, and farnesene did not show any activity. The compounds eugenol, carvacrol, and thymol presented the lowest MIC; bactericidal effect was observed at MIC level for S. aureus 75MR, E. coli 128 MR, and C albicans CUSI, for different compounds, eugenol, carvacrol, and thymol. Finally, this study shows that the essential oils of plants used by the population of Tehuacán-Cuicatlán valley share compounds and some of them have antibacterial and fungicidal activity.

scholarly journals Antibiotic sensitivities of Neisseria meningitidis isolates from patients and carriers in Greece

1992 ◽  
Vol 108 (3) ◽  
pp. 449-455 ◽  
Author(s):  
G. Tzanakaki ◽  
C. C. Blackwell ◽  
J. Kremastinou ◽  
C. Kallergi ◽  
G. Kouppari ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
School Children ◽  
Penicillin G ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Southern Europe ◽  
Agar Dilution ◽  
Of Greece

SUMMARYUsage of antibiotics in southern Europe is less well regulated than in northern countries. The proportion (48%) of meningococci in Spain insensitive to penicillin (MIC ≥ 0·1 mg/l) prompted this investigation of antibiotic sensitivities of isolates from Greek patients with meningitis (31) and carriers (47 school-children and 472 recruits). The agar dilution method was used to determine MIC to penicillin G (PN), sulphamethoxazole (SU), rifampicin (RF), cefaclor (CF) and ciprofloxacin (CP).The proportion of isolates insensitive to PN was 48% for isolates from patients, 19% from school-children and 36·6% from recruits. Resistance to SU (MIC ≥ 16 mg/l) was found in 16% of those from patients, 10·6% from children and 40% from recruits. None of the isolates from patients was resistant to RF (≥ 1 mg/l) but 6% of those from carriers were. Resistance to CF (≥ 4 mg/l) was found in 9·2% of patient isolates, 6·4% from children and 23·7% from recruits. All isolates except one were sensitive to CP (MIC range < 0·0015–0·125 mg/l).Resistances to PN, SU and RF were analysed by serogroup, serotype and subtype of the bacteria. The proportion of resistant isolates showed some variation between different areas of Greece, but it was not statistically significant.

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