Identification and possible role of a MYB transcription factor from saffron (Crocus sativus)

Abstract The members of myeloblastosis transcription factor (MYB TF) family are involved in the regulation of biotic and abiotic stresses in plants. However, the role of MYB TF in phosphorus remobilization remains largely unexplored. In the present study, we show that an R2R3 type MYB transcription factor, MYB103, is involved in phosphorus (P) remobilization. MYB103 was remarkably induced by P deficiency in cabbage (Brassica oleracea var. capitata L.). As cabbage lacks the proper mutant for elucidating the mechanism of MYB103 in P deficiency, another member of the crucifer family, Arabidopsis thaliana was chosen for further study. The transcript of its homologue AtMYB103 was also elevated in response to P deficiency in A. thaliana, while disruption of AtMYB103 (myb103) exhibited increased sensitivity to P deficiency, accompanied with decreased tissue biomass and soluble P concentration. Furthermore, AtMYB103 was involved in the P reutilization from cell wall, as less P was released from the cell wall in myb103 than in wildtype, coinciding with the reduction of ethylene production. Taken together, our results uncover an important role of MYB103 in the P remobilization, presumably through ethylene signaling.

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Expression of miR159 Is Altered in Tomato Plants Undergoing Drought Stress

Plants ◽

10.3390/plants8070201 ◽

2019 ◽

Vol 8 (7) ◽

pp. 201 ◽

Cited By ~ 3

Author(s):

María José López-Galiano ◽

Inmaculada García-Robles ◽

Ana I. González-Hernández ◽

Gemma Camañes ◽

Begonya Vicedo ◽

...

Keyword(s):

Transcription Factor ◽

Drought Stress ◽

Regulatory Networks ◽

Crop Yields ◽

Insect Pest ◽

Regulatory Function ◽

Myb Transcription Factor ◽

Regulation Of Transcription ◽

Tomato Plants

In a scenario of global climate change, water scarcity is a major threat for agriculture, severely limiting crop yields. Therefore, alternatives are urgently needed for improving plant adaptation to drought stress. Among them, gene expression reprogramming by microRNAs (miRNAs) might offer a biotechnologically sound strategy. Drought-responsive miRNAs have been reported in many plant species, and some of them are known to participate in complex regulatory networks via their regulation of transcription factors involved in water stress signaling. We explored the role of miR159 in the response of Solanum lycopersicum Mill. plants to drought stress by analyzing the expression of sly-miR159 and its target SlMYB transcription factor genes in tomato plants of cv. Ailsa Craig grown in deprived water conditions or in response to mechanical damage caused by the Colorado potato beetle, a devastating insect pest of Solanaceae plants. Results showed that sly-miR159 regulatory function in the tomato plants response to distinct stresses might be mediated by differential stress-specific MYB transcription factor targeting. sly-miR159 targeting of SlMYB33 transcription factor transcript correlated with accumulation of the osmoprotective compounds proline and putrescine, which promote drought tolerance. This highlights the potential role of sly-miR159 in tomato plants’ adaptation to water deficit conditions.

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The R2R3-MYB transcription factor family in Taxus chinensis: identification, characterization, expression profiling and posttranscriptional regulation analysis

PeerJ ◽

10.7717/peerj.8473 ◽

2020 ◽

Vol 8 ◽

pp. e8473

Author(s):

Xinling Hu ◽

Lisha Zhang ◽

Iain Wilson ◽

Fenjuan Shao ◽

Deyou Qiu

Keyword(s):

Transcription Factor ◽

Posttranscriptional Regulation ◽

Gene Families ◽

Myb Transcription Factor ◽

Taxus Chinensis ◽

Transcription Factor Family ◽

Myb Genes ◽

And Function ◽

R2r3 Myb

The MYB transcription factor family is one of the largest gene families playing regulatory roles in plant growth and development. The MYB family has been studied in a variety of plant species but has not been reported in Taxus chinensis. Here we identified 72 putative R2R3-MYB genes in T. chinensis using a comprehensive analysis. Sequence features, conversed domains and motifs were characterized. The phylogenetic analysis showed TcMYBs and AtMYBs were clustered into 36 subgroups, of which 24 subgroups included members from T. chinensis and Arabidopsis thaliana, while 12 subgroups were specific to one species. This suggests the conservation and specificity in structure and function of plant R2R3-MYBs. The expression of TcMYBs in various tissues and different ages of xylem were investigated. Additionally, miRNA-mediated posttranscriptional regulation analysis revealed that TcMYBs were the targets of miR858, miR159 and miR828, suggesting the posttranscriptional regulation of MYBs is highly conserved in plants. The results provide a basis for further study the role of TcMYBs in the regulation of secondary metabolites of T. chinensis.

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Essential role of MYB transcription factor: PvPHR1 and microRNA: PvmiR399 in phosphorus-deficiency signalling in common bean roots

Plant Cell & Environment ◽

10.1111/j.1365-3040.2008.01883.x ◽

2008 ◽

Vol 31 (12) ◽

pp. 1834-1843 ◽

Cited By ~ 110

Author(s):

OSWALDO VALDÉS-LÓPEZ ◽

CATALINA ARENAS-HUERTERO ◽

MARIO RAMÍREZ ◽

LOURDES GIRARD ◽

FEDERICO SÁNCHEZ ◽

...

Keyword(s):

Transcription Factor ◽

Common Bean ◽

Essential Role ◽

Phosphorus Deficiency ◽

Myb Transcription Factor

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An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1902771116 ◽

2019 ◽

Vol 117 (1) ◽

pp. 271-277 ◽

Cited By ~ 9

Author(s):

Jun He ◽

Yuqiang Liu ◽

Dingyang Yuan ◽

Meijuan Duan ◽

Yanling Liu ◽

...

Keyword(s):

Transcription Factor ◽

Phenylalanine Ammonia Lyase ◽

Brown Planthopper ◽

Rice Cultivar ◽

Phenylpropanoid Pathway ◽

Myb Transcription Factor ◽

Resistance Response ◽

Key Enzyme ◽

R2r3 Myb

Brown planthopper (BPH) is one of the most destructive insects affecting rice (Oryza sativaL.) production. Phenylalanine ammonia-lyase (PAL) is a key enzyme involved in plant defense against pathogens, but the role of PAL in insect resistance is still poorly understood. Here we show that expression of the majority ofPALsin rice is significantly induced by BPH feeding. Knockdown of OsPALssignificantly reduces BPH resistance, whereas overexpression ofOsPAL8in a susceptible rice cultivar significantly enhances its BPH resistance. We found thatOsPALsmediate resistance to BPH by regulating the biosynthesis and accumulation of salicylic acid and lignin. Furthermore, we show that expression ofOsPAL6andOsPAL8in response to BPH attack is directly up-regulated by OsMYB30, an R2R3 MYB transcription factor. Taken together, our results demonstrate that the phenylpropanoid pathway plays an important role in BPH resistance response, and provide valuable targets for genetic improvement of BPH resistance in rice.

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MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus

10.21203/rs.2.21480/v1 ◽

2020 ◽

Author(s):

pan zhang ◽

Xiaofeng Liu ◽

Xin Xu ◽

Fusheng Wang ◽

Junhong Long ◽

...

Keyword(s):

Transcription Factor ◽

Biosynthetic Pathway ◽

Developmental Stages ◽

Regulatory Mechanisms ◽

Myb Transcription Factor ◽

Transcription Activator ◽

Multiple Alignment Analysis ◽

Citrus Varieties ◽

Alignment Analysis

Abstract Backgrounds Limonoids, a major bioactive components, are produced by triterpenoids metabolic pathway. So far, the detailed biochemical reactions regarding to limonoid biosynthesis and their molecular regulation remain elusive. The identification of transcription factors that regulate limonoids biosynthetic pathways is not only necessary for understanding the regulatory mechanisms but also as a tool for manipulating biosynthetic genes for biotechnological applications.Results In this study, CiMYB42 transcription factor was isolated and identified. Multiple alignment analysis and phylogenetic analysis demonstrated that CiMYB42 is a typical R2R3MYB transcription factor and its amino acid is similar to that of AtMYB42 . The limonoids content was higher in the citrus sinensis and citrus grandis than other species. The diverse accumulation patterns also showed in different leaf developmental stages. Expression of CiMYB42 was significantly related to limonoids content and the expression of CiOSC in some of citrus varieties. CiMYB42 transgenic sweet orange resulted significantly change on limonoids contents. Noticeably, CiMYB42 -RNAi induced dwarf phenotype and mainly decreased nomilin accumulation. Overexpressing CiMYB42 mainly increased limonin content. Yeast one hybrid assay results indicated that CiMYB42 exclusively bind to the promoter of CiOSC. In brief, CiMYB42 involved in the limonoids biosynthesis through binding the promoter of CiOSC in citrus.Conclusions These results indicated that CiMYB42 is an important transcription activator involved in limonoids biosynthesis by regulating the expression of CiOSC . This is the first report elucidating the role of transcription factor in citrus limonoids biosynthesis. Our contributions will provide a reference to understanding regulatory mechanisms of R2R3MYB TFs in the triterpenoids biosynthetic pathway.

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