Expression of genes for the biosynthesis of compatible solutes during pollen development under heat stress in tomato (Solanum lycopersicum)

ABSTRACTMarine hyperthermophiles accumulate small organic compounds, known as compatible solutes, in response to supraoptimal temperatures or salinities.Pyrococcus furiosusis a hyperthermophilic archaeon that grows optimally at temperatures near 100°C. This organism accumulates mannosylglycerate (MG) and di-myo-inositol phosphate (DIP) in response to osmotic and heat stress, respectively. It has been assumed that MG and DIP are involved in cell protection; however, firm evidence for the roles of these solutes in stress adaptation is still missing, largely due to the lack of genetic tools to produce suitable mutants of hyperthermophiles. Recently, such tools were developed forP. furiosus, making this organism an ideal target for that purpose. In this work, genes coding for the synthases in the biosynthetic pathways of MG and DIP were deleted by double-crossover homologous recombination. The growth profiles and solute patterns of the two mutants and the parent strain were investigated under optimal growth conditions and also at supraoptimal temperatures and NaCl concentrations. DIP was a suitable replacement for MG during heat stress, but substitution of MG for DIP and aspartate led to less efficient growth under conditions of osmotic stress. The results suggest that the cascade of molecular events leading to MG synthesis is tuned for osmotic adjustment, while the machinery for induction of DIP synthesis responds to either stress agent. MG protects cells against heat as effectively as DIP, despite the finding that the amount of DIP consistently increases in response to heat stress in the nine (hyper)thermophiles examined thus far.

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Effects of long-term heat stress and dietary restriction on the expression of genes of steroidogenic pathway and small heat-shock proteins in rat testicular tissue

Andrologia ◽

10.1111/and.12668 ◽

2016 ◽

Vol 49 (6) ◽

pp. e12668 ◽

Cited By ~ 7

Author(s):

F. Bozkaya ◽

M. O. Atli ◽

A. Guzeloglu ◽

S. A. Kayis ◽

M. E. Yildirim ◽

...

Keyword(s):

Heat Stress ◽

Heat Shock ◽

Heat Shock Proteins ◽

Dietary Restriction ◽

Testicular Tissue ◽

Small Heat Shock Proteins ◽

Expression Of Genes ◽

Shock Proteins

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IRE1, a component of the unfolded protein response signaling pathway, protects pollen development in Arabidopsis from heat stress

The Plant Journal ◽

10.1111/tpj.13239 ◽

2016 ◽

Vol 88 (2) ◽

pp. 193-204 ◽

Cited By ~ 43

Author(s):

Yan Deng ◽

Renu Srivastava ◽

Teagen D. Quilichini ◽

Haili Dong ◽

Yan Bao ◽

...

Keyword(s):

Heat Stress ◽

Unfolded Protein Response ◽

Signaling Pathway ◽

Pollen Development ◽

Unfolded Protein ◽

The Unfolded Protein Response ◽

Protein Response

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Genetic analysis of stomatal conductance in upland cotton (Gossypium hirsutum L.) under contrasting temperature regimes

The Journal of Agricultural Science ◽

10.1017/s0021859605005186 ◽

2005 ◽

Vol 143 (2-3) ◽

pp. 161-168 ◽

Cited By ~ 10

Author(s):

H. U. RAHMAN

Keyword(s):

Heat Stress ◽

Stomatal Conductance ◽

Temperature Regime ◽

Upland Cotton ◽

Selection Efficiency ◽

Gossypium Hirsutum L ◽

The Third ◽

Expression Of Genes ◽

Temperature Regimes ◽

Genetically Determined

Stomatal conductance plays an important role in the heat avoidance mechanism of crop plants. Stomatal conductance in cotton is genetically determined and has been shown to be associated with heat resistance and higher yields. Experiments were carried out with six generations (parental, F1, F2 and back crosses) of three upland cotton crosses under heat-stressed and non-stressed greenhouse and field regimes, to understand the inheritance pattern of stomatal conductance as affected by contrasting temperature regimes. The results revealed significant variation for stomatal conductance due to generations and generation×temperature regime interaction in the three crosses. In general, heat stress reduced stomatal conductance and available genetic variability. Temperature regimes exerted a significant effect on the expression of the genes responsible for stomatal conductance. High temperature or heat stress favoured the expression of genes having additive effects, while absence of heat stress favoured those having dominant effects in two of the three crosses evaluated. The third cross showed the opposite reaction. The results suggest that genes controlling stomatal conductance in the parents of the first two crosses (MNH-552, HR109-RT, CIM-448, CRIS-19) were different from those controlling stomatal conductance in FH-900 and N-Karishma, the parents of the third cross. The selection efficiency of stomatal conductance in segregating populations was likely to be affected by the complexity of its inheritance, environmental dependency, and presence of substantial non-allelic and genotype×temperature regime interactions.

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Unique miRNAs and their targets in tomato leaf responding to combined drought and heat stress

10.21203/rs.2.14408/v3 ◽

2020 ◽

Author(s):

Rong Zhou ◽

Xiaqing Yu ◽

Carl-Otto Ottosen ◽

Tingling Zhang ◽

Zhen Wu ◽

...

Keyword(s):

Heat Stress ◽

Dna Binding ◽

Solanum Lycopersicum ◽

Target Genes ◽

Crop Improvement ◽

Regulation Of Transcription ◽

Solanum Lycopersicum L ◽

Activity Sequence ◽

Small Rna Deep Sequencing ◽

Drought And Heat Stress

Abstract Background: Both drought and heat stress are serious global problems, leading to agricultural production loss. MicroRNAs (miRNAs) play important roles in plant species responding to individual drought and heat stress. However, the miRNAs and mRNAs in association with combined drought and heat in crops like tomato remains unclear. Results: We studied the crosstalk of miRNAs and their target genes in tomato plants grown under simultaneous drought and heat stress that frequently happen in field conditions. In total, 335 known miRNAs representing 55 miRNA families and 430 potential novel miRNAs were identified in Solanum lycopersicum L. using small RNA deep sequencing. Through expression analysis, miRNAs in association with drought, heat and the combination of these were investigated. In total, 61, 74 and 37 miRNAs were differentially regulated for combination (of both stresses) vs control, combination vs drought and combination vs heat, respectively. Target genes with different expression levels were found using degradome sequencing, which were mainly involved in transcription factor activity, sequence-specific DNA binding, transcription, regulation of transcription, nucleus, DNA binding etc . The quantitative real-time polymerase chain reaction (qRT-PCR) results confirmed the accuracy of sequencing. Conclusions: Our study serves as valuable knowledge on how crop adapted to combined drought and heat stress by regulating miRNAs and mRNAs, which provide information for crop improvement to deal with future climate changes. Keywords: Solanum lycopersicum L.; miRNAs; degradome; functional analysis; combined abiotic stress

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