scholarly journals Male Sterility in Maize after Transient Heat Stress during the Tetrad Stage of Pollen Development

2019 ◽  
Vol 181 (2) ◽  
pp. 683-700 ◽  
Author(s):  
Kevin Begcy ◽  
Tetyana Nosenko ◽  
Liang-Zi Zhou ◽  
Lena Fragner ◽  
Wolfram Weckwerth ◽  
...  
Keyword(s):  
Heat Stress ◽  
Male Sterility ◽  
Pollen Development ◽  
Tetrad Stage

scholarly journals Comparative transcriptome analysis reveals key insights into male sterility in Salvia miltiorrhiza Bunge

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11326
Author(s):  
Yan Yu ◽  
Yuanyuan Jiang ◽  
Long Wang ◽  
Yichao Wu ◽  
Jinqiu Liao ◽  
...  
Keyword(s):  
Male Sterility ◽  
Differentially Expressed Genes ◽  
Transcriptome Analysis ◽  
Pollen Development ◽  
Salvia Miltiorrhiza ◽  
Comparative Transcriptome ◽  
Paraffin Sections ◽  
Tetrad Stage ◽  
Comparative Transcriptome Analysis ◽  
Go Terms

Background Large-scale heterosis breeding depends upon stable, inherited male sterility lines. We accidentally discovered a male sterility line (SW-S) in the F1progeny of a Salvia miltiorrhiza Bunge from Shandong, China (purple flowers) crossed with a S. miltiorrhiza f. alba from Sichuan, China (white flowers). We sought to provide insights into the pollen development for male sterility in S. miltiorrhiza. Methods The phenotypic and cytological features of the SW-S and fertile control SW-F were observed using scanning electron microscopy and paraffin sections to identify the key stage of male sterility. Transcriptome profiles were recorded for anthers at the tetrad stage of SW-S and SW-F using Illumina RNA-Seq. Results The paraffin sections showed that sterility mainly occurred at the tetrad stage of microspore development, during which the tapetum cells in the anther compartment completely fell off and gradually degraded in the sterile line. There was little-to-no callose deposited around the microspore cells. The tetrad microspore was shriveled and had abnormal morphology. Therefore, anthers at the tetrad stage of SW-S and fertile control SW-F were selected for comparative transcriptome analysis. In total, 266,722,270 clean reads were obtained from SW-S and SW-F, which contained 36,534 genes. There were 2,571 differentially expressed genes (DEGs) in SW-S and SW-F, of which 63.5% were downregulated. Gene Ontology (GO) enrichment analysis indicated that the differentially expressed genes were enriched in 56 functional groups (GO terms); of these, all DEGs involved in microgametogenesis and developmental maturation were downregulated in SW-S. These results were confirmed by quantitative RT-PCR. The two GO terms contained 18 DEGs, among which eight DEGs (namely: GPAT3, RHF1A, phosphatidylinositol, PFAS, MYB96, MYB78, Cals5, and LAT52) were related to gamete development. There were 10 DEGs related to development and maturation, among which three genes were directly related to pollen development (namely: ACT3, RPK2, and DRP1C). Therefore, we believe that these genes are directly or indirectly involved in the pollen abortion of SW-S. Our study provides insight into key genes related to sterility traits in S. miltiorrhiza, and the results can be further exploited in functional and mechanism studies.

scholarly journals High Temperatures Cause Male Sterility in Rice Plants with Transcriptional Alterations During Pollen Development

2009 ◽  
Vol 50 (11) ◽  
pp. 1911-1922 ◽  
Author(s):  
Makoto Endo ◽  
Tohru Tsuchiya ◽  
Kazuki Hamada ◽  
Shingo Kawamura ◽  
Kentaro Yano ◽  
...  
Keyword(s):  
Male Sterility ◽  
High Temperatures ◽  
Pollen Development ◽  
Rice Plants ◽  
Transcriptional Alterations

scholarly journals Identification and Verification of Genes Related to Pollen Development and Male Sterility Induced by High Temperature in Thermo-sensitive Genic Male Sterile Wheat Line YanZhan 4110S

2020 ◽  
Author(s):  
Xuetong Yang ◽  
Jiali Ye ◽  
Fuqiang Niu ◽  
Yi Feng ◽  
Xiyue Song
Keyword(s):  
Gene Silencing ◽  
Male Sterility ◽  
Mosaic Virus ◽  
Pollen Development ◽  
Bioinformatics Analysis ◽  
Virus Induced Gene Silencing ◽  
Wheat Line ◽  
Pollen Abortion ◽  
Hub Genes ◽  
Genic Male Sterility

Abstract Background: Environment-sensitive genic male sterility is of vital importance to hybrid vigor in crop production and breeding, therefore, it is meaningful to identify and study the function of the genes related to pollen development and male sterility, which still not fully understanding currently. In this study, Yanzhan 4110S, a new thermo-sensitive genic male sterility (TGMS) wheat line, and its near isogenic line Yanzhan 4110 were carried out cytological features observation, bioinformatics analysis to investgate the abortion state and identified the genes involved in pollen development which have fertility regulation function. Barely stripe mosaic virus-induced gene silencing was used to verify the genes function.Results: Cytological analysis showed pollen abortion event of Yanzhan 4110S occur at the later uninucleate stage (Lun) under higher temperature induction (day/night temperatures of 22 °C/20 °C), when the anthers were collected and assessed for transcriptomic profiling through high-throughput sequencing. We then in-depth analyzed the differentially expressed genes (DEGs) by Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, the results showed that the occurrence of Yanzhan 4110S male-sterility most likely related to metabolic pathway, including phenylpropanoid biosynthesis in the biosynthesis of other secondary metabolites, starch and sucrose metabolism in carbohydrate metabolism, carbon fixation in photosynthetic organisms as well as carbon metabolism in energy metabolism. The weighted gene co-expression network analysis in the transcriptome profiles further identified some hub genes, where the key genes involved in those pathways were intersection between the unique DEGs of Yanzhan 4110S in anther and hub genes, totally 228 genes, which were highly related to pollen development including TaMut11 and TaSF3. Moreover, further verification through barely stripe mosaic virus-induced gene silencing elucidated that the silencing of TaMut11 and TaSF3 caused pollen abortion, finally resulting in the declination of fertility. So, the genes TaMut11 and TaSF3 are related to fertility conversion of Yanzhan 4110S.Conclusion: Through comparative transcriptome bioinformatics analysis, the genes TaMut11 and TaSF3 associated with pollen development and male sterility induced by high temperature were identified in Yanzhan 4110S, and verificated by barely stripe mosaic virus-induced gene silencing. These findings provided researching the abortive mechanism in environment-sensitive genic male sterility wheat.

Mechanisms of Male Sterility in Four Cryptomeria japonica Individuals with Obvious Visible Abnormality at the Tetrad Stage.

2011 ◽  
Vol 93 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Saori Miura ◽  
Masaaki Nameta ◽  
Tadashi Yamamoto ◽  
Masanori Igarashi ◽  
Hideaki Taira
Keyword(s):  
Male Sterility ◽  
Cryptomeria Japonica ◽  
Tetrad Stage

scholarly journals Increased expression of the MALE STERILITY1 transcription factor gene results in temperature-sensitive male sterility in barley

2020 ◽  
Vol 71 (20) ◽  
pp. 6328-6339
Author(s):  
José Fernández-Gómez ◽  
Behzad Talle ◽  
Zoe A Wilson
Keyword(s):  
Transcription Factor ◽  
Male Sterility ◽  
Pollen Development ◽  
Breeding Systems ◽  
Hybrid Breeding ◽  
Temperature Sensitive ◽  
Hybrid Vigour ◽  
Male Sterile ◽  
The Impact

Abstract Understanding the control of fertility is critical for crop yield and breeding; this is particularly important for hybrid breeding to capitalize upon the resultant hybrid vigour. Different hybrid breeding systems have been adopted; however, these are challenging and crop specific. Mutants with environmentally reversible fertility offer valuable opportunities for hybrid breeding. The barley HvMS1 gene encodes a PHD-finger transcription factor that is expressed in the anther tapetum, which is essential for pollen development and causes complete male sterility when overexpressed in barley. This male sterility is due at least in part to indehiscent anthers resulting from incomplete tapetum degeneration, failure of anther opening, and sticky pollen under normal growth conditions (15 °C). However, dehiscence and fertility are restored when plants are grown at temperatures >20 °C, or when transferred to >20 °C during flowering prior to pollen mitosis I, with transfer at later stages unable to rescue fertility in vivo. As far as we are aware, this is the first report of thermosensitive male sterility in barley. This offers opportunities to understand the impact of temperature on pollen development and potential applications for environmentally switchable hybrid breeding systems; it also provides a ‘female’ male-sterile breeding tool that does not need emasculation to facilitate backcrossing.

scholarly journals Comparative profile analysis reveals differentially expressed microRNAs regulate anther and pollen development in kenaf cytoplasmic male sterility line

Genome ◽  
2019 ◽  
Vol 62 (7) ◽  
pp. 455-466
Author(s):  
Peng Chen ◽  
Qiqi Shi ◽  
Zhichen Liang ◽  
Hai Lu ◽  
Ru Li
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Regulatory Network ◽  
Pollen Development ◽  
Protein Modification ◽  
Target Genes ◽  
Profile Analysis ◽  
Differentially Expressed ◽  
Auxin Signaling ◽  
Mirna Regulation

Cytoplasmic male sterility (CMS) is advantageous in extensive crop breeding and represents a perfect model for understanding anther and pollen development research. MicroRNAs (miRNAs) play key roles in regulating various biological processes. However, the miRNA-mediated regulatory network in kenaf CMS occurrence remains largely unknown. In the present study, a comparative deep sequencing approach was used to investigate the miRNAs and their roles in regulating anther and pollen development during CMS occurrence. We identified 283 known and 46 new candidate miRNAs in kenaf anther. A total of 67 differentially expressed miRNAs (DEMs) were discovered between CMS and its maintainer line. Among them, 40 and 27 miRNAs were up- and downregulated, respectively. These 67 DEMs were predicted to target 189 genes. Validation of DEMs and putative target genes were confirmed by using real-time quantitative PCR. In addition, a potential miRNA-mediated regulatory network, which mainly involves the auxin signaling pathway, signal transduction, glycolysis and energy metabolism, gene expression, transmembrane transport, protein modification and metabolism, and floral development, that mediates anther development during CMS occurrence was proposed. Taken together, our findings provide a better understanding of the molecular mechanism of miRNA regulation in pollen development and CMS occurrence in kenaf.

An ultrastructural study of pollen development in tomato (Lycopersicon esculentum). I. Tetrad to early binucleate microspore stage

1993 ◽  
Vol 71 (8) ◽  
pp. 1039-1047 ◽  
Author(s):  
P. L. Polowick ◽  
V. K. Sawhney
Keyword(s):  
Endoplasmic Reticulum ◽  
Lycopersicon Esculentum ◽  
Ultrastructural Study ◽  
Pollen Development ◽  
Pollen Wall ◽  
Ultrastructural Changes ◽  
Dense Matrix ◽  
Tetrad Stage ◽  
Microspore Stage ◽  
Electron Dense Matrix

Microspores undergo considerable ultrastructural changes between the tetrad and early binucleate microspore stages of microsporogenesis in tomato (Lycopersicon esculentum). Pollen wall deposition began late in the tetrad stage, and by the early microspore stage a lamellar foot layer and tectum were deposited. Sculpturing of the tectum was evident by the early binucleate microspore stage. Dictyosomes and vesicles were abundant during the period of pollen wall formation. Plastids were associated with the endoplasmic reticulum (ER) to form plastid–ER complexes, from the late tetrad to the vacuolate microspore stage. At the vacuolate microspore stage, endoplasmic reticulum independent of plastids was also observed, and at the early binucleate microspore stage ER was not associated with plastids. Free ribosomes were evenly distributed throughout the cytoplasm until the vacuolate microspore stage when they were organized into polysomes. Mitochondria were spherical to ellipsoid, with an electron-dense matrix and swollen cristae, until the early binucleate microspore stage when they were highly elongate and became convoluted. Key words: Lycopersicon esculentum, microsporogenesis, pollen development, tetrads, tomato, ultrastructure.

THE EFFECT OF TEMPERATURE ON MEIOSIS AND POLLEN DEVELOPMENT IN WHEAT AND RYE

1972 ◽  
Vol 14 (3) ◽  
pp. 615-624 ◽  
Author(s):  
M. D. Bennett ◽  
J. B. Smith ◽  
R. Kemble
Keyword(s):  
Male Sterility ◽  
Chinese Spring ◽  
Pollen Development ◽  
Effect Of Temperature ◽  
Maturation Time ◽  
Pollen Maturation ◽  
Cereal Breeding ◽  
Chinese Spring Wheat ◽  
Increasing Temperature ◽  
Temperature Responses

The durations of meiosis and pollen development were estimated in Petkus Spring rye and Chinese Spring wheat grown at 15, 20 and 25 °C. Over the range 15-25 °C, meiotic duration and pollen maturation time decreased with increasing temperature and the ratio between the durations of these stages at 25 °C and at 15 °C (Q10) indicated that the temperature responses of meiotic and pollen development were similar in both species. Both within and between species meiosis and pollen maturation had constant relative durations at each temperature. The effect of low temperature on the duration of meiosis and pollen development was similar in Holdfast, an extreme type of winter wheat, as in Chinese Spring. The relevance of these results to hybrid cereal breeding are mentioned. In plants grown at 25 °C, abnormal meiotic and pollen development resulting in male sterility was seen in some anthers. The cause of male sterility appeared to be thickening of tapetal cell walls concurrent with abnormal late meiotic development in PMC's.

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