Differential secretome profiling of a swine tracheal cell line infected with mycoplasmas of the swine respiratory tract

Human rhinoviruses (HRV) are responsible for the majority of virus infections of the upper respiratory tract. Furthermore, HRV infection is associated with acute exacerbation of asthma and other chronic respiratory diseases of the lower respiratory tract. A small animal model of HRV-induced disease is required for the development of new therapies. However, existing mouse models of HRV infection are difficult to work with and until recently mouse cell lines were thought to be generally non-permissive for HRV replication in vitro. In this report we demonstrate that a virus of the minor receptor group, HRV1B, can infect and replicate in a mouse respiratory epithelial cell line (LA-4) more efficiently than in a mouse fibroblast cell line (L). The major receptor group virus HRV16 requires human intercellular adhesion molecule-1 (ICAM-1) for cell entry and therefore cannot infect LA-4 cells. However, transfection of in vitro-transcribed HRV16 RNA resulted in the replication of viral RNA and production of infectious virus. Expression of a chimeric ICAM-1 molecule, comprising mouse ICAM-1 with extracellular domains 1 and 2 replaced by the equivalent human domains, rendered the otherwise non-permissive mouse respiratory epithelial cell line susceptible to entry and efficient replication of HRV16. These observations suggest that the development of mouse models of respiratory tract infection by major as well as minor group HRV should be pursued.

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Role of Bordetella pertussis Virulence Factors in Adherence to Epithelial Cell Lines Derived from the Human Respiratory Tract

Infection and Immunity ◽

10.1128/iai.67.3.1056-1062.1999 ◽

1999 ◽

Vol 67 (3) ◽

pp. 1056-1062 ◽

Cited By ~ 62

Author(s):

Bernard M. van den Berg ◽

Henry Beekhuizen ◽

Rob J. L. Willems ◽

Frits R. Mooi ◽

Ralph van Furth

Keyword(s):

Epithelial Cell ◽

Epithelial Cells ◽

Respiratory Tract ◽

Cell Line ◽

Virulence Factors ◽

Bordetella Pertussis ◽

Epithelial Cell Line ◽

Human Respiratory Tract ◽

Bronchial Epithelial ◽

Laryngeal Mucosa

ABSTRACT During colonization of the respiratory tract by Bordetella pertussis, virulence factors contribute to adherence of the bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the adherence of B. pertussis to cells of the human bronchial epithelial cell line NCI-H292 and of the laryngeal epithelial cell line HEp-2. Using B. pertussis mutant strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that both fimbriae and FHA are involved in the adhesion of B. pertussis to laryngeal epithelial cells, whereas only FHA is involved in the adherence to bronchial epithelial cells. For PT and Prn, no role as adhesion factor was found. However, purified PT bound to both bronchial and laryngeal cells and as such reduced the adherence of B. pertussis to these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract.

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Anti-Viral Activity of Water Extract of Paeonia lactiflora Pallas Against Human Respiratory Syncytial Virus in Human Respiratory Tract Cell Lines

The American Journal of Chinese Medicine ◽

10.1142/s0192415x13500419 ◽

2013 ◽

Vol 41 (03) ◽

pp. 585-599 ◽

Cited By ~ 8

Author(s):

Tzeng-Jih Lin ◽

Kuo-Chih Wang ◽

Chun-Ching Lin ◽

Lien-Chai Chiang ◽

Jung-San Chang

Keyword(s):

Respiratory Syncytial Virus ◽

Respiratory Tract ◽

Cell Line ◽

Carcinoma Cell ◽

A549 Cells ◽

Water Extract ◽

Human Respiratory Syncytial Virus ◽

Paeonia Lactiflora ◽

Viral Attachment ◽

Syncytial Virus

Paeonia lactiflora Pallas (P. lactiflora, Ranunculaceae) is a common ingredient of Sheng-Ma-Ge-Gen-Tang (SMGGT; Shoma-kakkon-to) and Ge-Gen-Tang (GGT; kakkon-to). SMGGT and GGT are different prescriptions of traditional Chinese medicine with different ingredients designed for airway symptoms. Both SMGGT and GGT have anti-viral activity against human respiratory syncytial virus (HRSV). Therefore, P. lactiflora was hypothesized to be the effective ingredient of both SMGGT and GGT against HRSV. However, P. lactiflora does not have any proven antiviral activity. This study used both human upper (Human larynx epidermoid carcinoma cell line, HEp-2) and lower (human lung carcinoma cell line, A549) respiratory tract cells to test the hypothesis that a hot water extract of P. lactiflora could effectively inhibit plaque formation induced by HRSV infection. The ability of P. lactiflora to stimulate anti-viral cytokines was evaluated by enzyme-linked immunosorbent assay (ELISA). The results showed that P. lactiflora was time-dependently and dose-dependently effective against HRSV in HEp-2 and A549 cells, particularly supplemented before viral inoculation (p < 0.0001). 10 μg/ml P. lactiflora had a comparable anti-HRSV activity with 10 μg/ml ribavirin, a broad-spectrum antiviral agent. P. lactiflora was dose-dependently effective against viral attachment (p < 0.0001), with a better effect on A549 cells (p < 0.0001). P. lactiflora was time-dependently (p < 0.0001) and dose-dependently (p < 0.0001) effective against viral penetration. Moreover, P. lactiflora stimulated IFN-β secretion without any effect on TNF-α secretion. Therefore, P. lactiflora could be beneficial at preventing HRSV infection by inhibiting viral attachment, internalization, and stimulating IFN secretion.

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Ultrastructure of NPC/HK1 cells heterotransplanted in athymic nude mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010005384x ◽

1982 ◽

Vol 40 ◽

pp. 236-237

Author(s):

E.C. Chew ◽

C.L. Li ◽

D.P. Huang ◽

H.C. Ho ◽

L.S. Mak ◽

...

Keyword(s):

Cell Line ◽

Nude Mice ◽

Biopsy Specimen ◽

Epithelial Cell Line ◽

Present Communication ◽

Recurrent Tumour ◽

Athymic Nude Mice ◽

Cell Line Establishment ◽

Fine Structures ◽

Well Differentiated

An epithelial cell line, NPC/HK1, has recently been established from a biopsy specimen of a recurrent tumour of the nasopharynx which was histologically diagnosed as a moderately to well differentiated squamous cell carcinoma. A definite decrease in the amount of tonofilaments and desmosomes in the NPC/HK1 cells during the cell line establishment was observed. The present communication reports on the fine structures of the NPC/HK1 cells heterotraneplanted in athymic nude mice.

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Ultrastructure of Choriocarcinoma in Vitro

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006372x ◽

1969 ◽

Vol 27 ◽

pp. 362-363

Author(s):

John C. Garancis ◽

R. A. Pattillo

Keyword(s):

Cell Line ◽

Physiological Function ◽

Cell System ◽

Bewo Cell ◽

Bewo Cells ◽

Gestational Choriocarcinoma ◽

Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

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An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053784 ◽

1982 ◽

Vol 40 ◽

pp. 224-225

Author(s):

Li C.L. ◽

Chew E.C. ◽

Huang D.P. ◽

Ho H.C. ◽

Mak L.S. ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Epithelial Cell ◽

Surface Morphology ◽

Cell Line ◽

Epithelial Cell Line ◽

Present Communication ◽

Cells In Culture ◽

Sem Study ◽

Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

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