T cell cytokine responses to stimulation with Ureaplasma parvum in pregnancy

2016 ◽  
Vol 116 ◽  
pp. 93-97 ◽  
Author(s):  
Yael D. Friedland ◽  
Tracey F. Lee-Pullen ◽  
Elizabeth A. Nathan ◽  
Rory Watts ◽  
Jeffrey A. Keelan ◽  
...  
Keyword(s):  
T Cell ◽  
Ureaplasma Parvum ◽  
Cytokine Responses ◽  
In Pregnancy

scholarly journals Modulation of CD4+ and CD8+ T Cell Function and Cytokine Responses in Strongyloides stercoralis Infection by Interleukin-27 (IL-27) and IL-37

2017 ◽  
Vol 85 (11) ◽  
Author(s):  
Rajamanickam Anuradha ◽  
Saravanan Munisankar ◽  
Yukthi Bhootra ◽  
Chandrakumar Dolla ◽  
Paul Kumaran ◽  
...  
Keyword(s):  
T Cell ◽  
Whole Blood ◽  
Strongyloides Stercoralis ◽  
T Cell Response ◽  
T Cell Subsets ◽  
Content Type ◽  
Antigen Stimulation ◽  
Parasite Antigen ◽  
Cytokine Responses ◽  
Interleukin 27

ABSTRACT Strongyloides stercoralis infection is associated with diminished antigen-specific Th1- and Th17-associated responses and enhanced Th2-associated responses. Interleukin-27 (IL-27) and IL-37 are two known anti-inflammatory cytokines that are highly expressed in S. stercoralis infection. We therefore wanted to examine the role of IL-27 and IL-37 in regulating CD4+ and CD8+ T cell responses in S. stercoralis infection. To this end, we examined the frequency of Th1/Tc1, Th2/Tc2, Th9/Tc9, Th17/Tc17, and Th22/Tc22 cells in 15 S. stercoralis-infected individuals and 10 uninfected individuals stimulated with parasite antigen following IL-27 or IL-37 neutralization. We also examined the production of prototypical type 1, type 2, type 9, type 17, and type 22 cytokines in the whole-blood supernatants. Our data reveal that IL-27 or IL-37 neutralization resulted in significantly enhanced frequencies of Th1/Tc1, Th2/Tc2, Th17/Tc17, Th9, and Th22 cells with parasite antigen stimulation. There was no induction of any T cell response in uninfected individuals following parasite antigen stimulation and IL-27 or IL-37 neutralization. Moreover, we also observed increased production of gamma interferon (IFN-γ), IL-5, IL-9, IL-17, and IL-22 and decreased production of IL-10 following IL-27 and IL-37 neutralization and parasite antigen stimulation in whole-blood cultures. Thus, we demonstrate that IL-27 and IL-37 limit the induction of particular T cell subsets along with cytokine responses in S. stercoralis infections, which suggest the importance of IL-27 and IL-37 in immune modulation in a chronic helminth infection.

scholarly journals Cervix-on-a-chip for investigating ascending Ureaplasma parvum infection in pregnancy

2022 ◽  
Vol 226 (1) ◽  
pp. S12-S13
Author(s):  
Ourlad Alzeus G. Tantengco ◽  
Lauren Richardson ◽  
Enkhtuya Radnaa ◽  
Ananth Kumar Kammala ◽  
Talar Kechichian ◽  
...  
Keyword(s):  
Ureaplasma Parvum ◽  
In Pregnancy

431 T CELL PROLIFERATIVE AND CYTOKINE RESPONSES TO ISLET PROTEINS IN AUTOANTIBODY NEGATIVE TYPE 2 PATIENTS

2005 ◽  
Vol 53 (1) ◽  
pp. S153.6-S154 ◽  
Author(s):  
A. Goel ◽  
B. Brooks-Worrell ◽  
H. Chiu ◽  
J. P. Palmer
Keyword(s):  
T Cell ◽  
Negative Type ◽  
Cytokine Responses

scholarly journals Expanding Clinical Phenotype and Novel Insights into the Pathogenesis of ICOS Deficiency

2019 ◽  
Vol 40 (2) ◽  
pp. 277-288 ◽  
Author(s):  
Hassan Abolhassani ◽  
Yasser M. El-Sherbiny ◽  
Gururaj Arumugakani ◽  
Clive Carter ◽  
Stephen Richards ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Cytotoxic T Lymphocyte ◽  
Clinical Phenotype ◽  
Compound Heterozygous ◽  
Dominant Phenotype ◽  
Cytokine Responses ◽  
Whole Exome ◽  
Stimulation Of

Abstract Background Inducible T cell co-stimulator (ICOS) deficiency has been categorized as a combined immunodeficiency often complicated by enteropathies, autoimmunity, lymphoproliferation, and malignancy. We report seven new patients and four novel ICOS mutations resulting in a common variable immunodeficiency (CVID)–like phenotype and show that dysregulated IL-12 release, reduced cytotoxic T lymphocyte–associated protein 4 (CTLA4) expression, and skewing towards a Th1-dominant phenotype are all associated with inflammatory complications in this condition. Methods A combination of whole exome and Sanger sequencing was used to identify novel mutations. Standard clinical and immunological evaluation was performed. FACS and ELISA-based assays were used to study cytokine responses and ICOS/ICOSL/CTLA4 expression following stimulation of whole blood and PBMCs with multiple TLR ligands, anti-CD3, and PHA. Results Four novel ICOS mutations included homozygous c.323_332del, homozygous c.451C>G, and compound heterozygous c.58+1G>A/c.356T>C. The predominant clinical phenotype was that of antibody deficiency associated with inflammatory complications in 4/7 patients. Six out of seven patients were treated with immunoglobulin replacement and one patient died from salmonella sepsis. All patients who were tested showed reduced IL-10 and IL-17 cytokine responses, normal IL-1β, IL6, and TNF release following LPS stimulation and highly elevated IL-12 production in response to combined LPS/IFNγ stimulation. This was associated with skewing of CD4+ T cells towards Th1 phenotype and increased expression of ICOSL on monocytes. Lastly, reduced CTLA4 expression was found in 2 patients. One patient treated with ustekinumab for pancytopenia due to granulomatous bone marrow infiltration failed to respond to this targeted therapy. Conclusions ICOS deficiency is associated with defective T cell activation, with simultaneously enhanced stimulation of monocytes. The latter is likely to result from a lack of ICOS/ICOSL interaction which might be necessary to provide negative feedback which limits monocytes activation.

scholarly journals Natural killer/T-cell lymphoma and secondary haemophagocytic lymphohistiocytosis in pregnancy

2018 ◽  
pp. bcr-2018-224832
Author(s):  
Barbara Neistadt ◽  
Aakriti Carrubba ◽  
Michael V Zaretksy
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
Multiorgan Failure ◽  
Male Infant ◽  
Fetal Distress ◽  
Chemotherapeutic Agents ◽  
T Cell Lymphoma ◽  
Haemophagocytic Lymphohistiocytosis ◽  
Aged Woman ◽  
In Pregnancy

Haemophagocytic lymphohistiocytosis (HLH) is a rare and potentially fatal disorder. It is challenging to diagnose due to its rarity and variation in clinical presentation, laboratory abnormalities and underlying aetiologies. A reproductive-aged woman, gravida 2 para 1001 at 27 weeks gestation presented with fever, hypotension and subacute upper respiratory infection. She delivered a male infant by caesarean section secondary to fetal distress. Subsequently, she was diagnosed with T-cell lymphoma and secondary HLH. Despite management with supportive care and multiple chemotherapeutic agents, she ultimately died of multiorgan failure. Patients with HLH secondary to malignancy have a particularly poor prognosis. This case highlights the importance of considering secondary HLH in the differential diagnosis of a patient with fever, pancytopenia and systemic symptoms of unclear aetiology in pregnancy.

scholarly journals Role of T cell TGF-β signaling in intestinal cytokine responses and helminthic immune modulation

2009 ◽  
Vol 39 (7) ◽  
pp. 1870-1878 ◽  
Author(s):  
M. Nedim Ince ◽  
David E. Elliott ◽  
Tommy Setiawan ◽  
Ahmed Metwali ◽  
Arthur Blum ◽  
...  
Keyword(s):  
T Cell ◽  
Immune Modulation ◽  
Cytokine Responses
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