Status of Oxidative Stress in Patients With Renal Cell Carcinoma

Mitochondria not only are the main source of ATP synthesis but also regulate cellular redox balance and calcium homeostasis. Its dysfunction can lead to a variety of diseases and promote cancer and metastasis. In this study, we aimed to explore the molecular characteristics and prognostic significance of mitochondrial genes (MTGs) related to oxidative stress in clear cell renal cell carcinoma (ccRCC). A total of 75 differentially expressed MTGs were analyzed from The Cancer Genome Atlas (TCGA) database, including 46 upregulated and 29 downregulated MTGs. Further analysis screened 6 prognostic-related MTGs (ACAD11, ACADSB, BID, PYCR1, SLC25A27, and STAR) and was used to develop a signature. Kaplan-Meier survival and receiver operating characteristic (ROC) curve analyses showed that the signature could accurately distinguish patients with poor prognosis and had good individual risk stratification and prognostic potential. Stratified analysis based on different clinical variables indicated that the signature could be used to evaluate tumor progression in ccRCC. Moreover, we found that there were significant differences in immune cell infiltration between the low- and high-risk groups based on the signature and that ccRCC patients in the low-risk group responded better to immunotherapy than those in the high-risk group (46.59% vs 35.34%, P = 0.008 ). We also found that the expression levels of these prognostic MTGs were significantly associated with drug sensitivity in multiple ccRCC cell lines. Our study for the first time elucidates the biological function and prognostic significance of mitochondrial molecules associated with oxidative stress and provides a new protocol for evaluating treatment strategies targeting mitochondria in ccRCC patients.

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Oxidative stress and DNA hypermethylation status in renal cell carcinoma arising in patients on dialysis

The Journal of Pathology ◽

10.1002/path.2176 ◽

2007 ◽

Vol 212 (2) ◽

pp. 218-226 ◽

Cited By ~ 32

Author(s):

Y Hori ◽

Y Oda ◽

K Kiyoshima ◽

Y Yamada ◽

Y Nakashima ◽

...

Keyword(s):

Oxidative Stress ◽

Renal Cell Carcinoma ◽

Cell Carcinoma ◽

Renal Cell ◽

Dna Hypermethylation

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Renal cell carcinoma and oxidative stress: The lack of peroxisomes

Acta Histochemica ◽

10.1016/j.acthis.2009.03.003 ◽

2010 ◽

Vol 112 (4) ◽

pp. 364-371 ◽

Cited By ~ 34

Author(s):

Wilma M. Frederiks ◽

Klazina S. Bosch ◽

Kees A. Hoeben ◽

Jan van Marle ◽

Sigrun Langbein

Keyword(s):

Oxidative Stress ◽

Renal Cell Carcinoma ◽

Cell Carcinoma ◽

Renal Cell ◽

And Oxidative Stress

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Vasoactive intestinal peptide induces oxidative stress and suppresses metastatic potential in human clear cell renal cell carcinoma

Molecular and Cellular Endocrinology ◽

10.1016/j.mce.2012.10.021 ◽

2013 ◽

Vol 365 (2) ◽

pp. 212-222 ◽

Cited By ~ 13

Author(s):

Eva Vacas ◽

Ana M. Bajo ◽

Andrew V. Schally ◽

Manuel Sánchez-Chapado ◽

Juan C. Prieto ◽

...

Keyword(s):

Oxidative Stress ◽

Renal Cell Carcinoma ◽

Cell Carcinoma ◽

Vasoactive Intestinal Peptide ◽

Renal Cell ◽

Clear Cell ◽

Metastatic Potential ◽

Cell Renal Cell Carcinoma

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Oxidative Stress Regulates Expression of VEGFR1 in Myeloid Cells: Link to Tumor-Induced Immune Suppression in Renal Cell Carcinoma

The Journal of Immunology ◽

10.4049/jimmunol.181.1.346 ◽

2008 ◽

Vol 181 (1) ◽

pp. 346-353 ◽

Cited By ~ 159

Author(s):

Sergei Kusmartsev ◽

Evgeniy Eruslanov ◽

Hubert Kübler ◽

Timothy Tseng ◽

Yoshihisa Sakai ◽

...

Keyword(s):

Oxidative Stress ◽

Renal Cell Carcinoma ◽

Cell Carcinoma ◽

Immune Suppression ◽

Renal Cell ◽

Myeloid Cells

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Effects of Open versus Laparoscopic Nephrectomy Techniques on Oxidative Stress Markers in Patients with Renal Cell Carcinoma

Oxidative Medicine and Cellular Longevity ◽

10.1155/2013/438321 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

Celestyna Mila-Kierzenkowska ◽

Alina Woźniak ◽

Tomasz Drewa ◽

Bartosz Woźniak ◽

Michał Szpinda ◽

...

Keyword(s):

Oxidative Stress ◽

Renal Cell Carcinoma ◽

Cell Carcinoma ◽

Renal Cell ◽

Open Surgery ◽

Acute Phase Proteins ◽

Thiobarbituric Acid ◽

Laparoscopic Nephrectomy ◽

Stress Markers ◽

Open Versus

The aim of the study was to determine the concentration of lipid peroxidation products, the activity of selected antioxidant and lysosomal enzymes, and protease inhibitor in patients with renal cell carcinoma who underwent radical nephrectomy. The studied group included 44 patients: 21 of them underwent open surgery, while 23 underwent laparoscopy. Blood samples were collected three times: before treatment and 12 hours and five days after nephrectomy. In blood of participants, the concentration of thiobarbituric acid reactive substances (TBARS), the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx), and the activity of acid phosphatase (AcP), arylsulfatase (ASA), cathepsin D (CTSD), andα1-antitrypsin (AAT) were assayed. No statistically significant differences in investigated parameters were found between studied groups. Moreover, TBARS concentration and CAT, SOD, and GPx activity were not altered in the course of both types of surgery. Five days after both open and laparoscopic nephrectomy techniques, AAT activity was higher than its activity 12 hours after the procedure. The obtained results suggest that laparoscopy may be used for nephrectomy as effectively as open surgery without creating greater oxidative stress. Reduced period of convalescence at patients treated with laparoscopy may be due to less severe response of acute-phase proteins.

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