Gene expression and oxidative stress markers profile associated with toxic metals in patients with renal cell carcinoma

2021 ◽  
Author(s):  
Heba H. Tarabay ◽  
Hassan Abol-Enein ◽  
Amira Awadalla ◽  
Wael I. Mortada ◽  
A. F. Abdel-Aziz
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Toxic Metals ◽  
Renal Cell ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
And Oxidative Stress

Renal cell carcinoma and oxidative stress: The lack of peroxisomes

2010 ◽  
Vol 112 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Wilma M. Frederiks ◽  
Klazina S. Bosch ◽  
Kees A. Hoeben ◽  
Jan van Marle ◽  
Sigrun Langbein
Keyword(s):  
Oxidative Stress ◽  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
And Oxidative Stress

scholarly journals Effects of Open versus Laparoscopic Nephrectomy Techniques on Oxidative Stress Markers in Patients with Renal Cell Carcinoma

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Celestyna Mila-Kierzenkowska ◽  
Alina Woźniak ◽  
Tomasz Drewa ◽  
Bartosz Woźniak ◽  
Michał Szpinda ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Open Surgery ◽  
Acute Phase Proteins ◽  
Thiobarbituric Acid ◽  
Laparoscopic Nephrectomy ◽  
Stress Markers ◽  
Open Versus

The aim of the study was to determine the concentration of lipid peroxidation products, the activity of selected antioxidant and lysosomal enzymes, and protease inhibitor in patients with renal cell carcinoma who underwent radical nephrectomy. The studied group included 44 patients: 21 of them underwent open surgery, while 23 underwent laparoscopy. Blood samples were collected three times: before treatment and 12 hours and five days after nephrectomy. In blood of participants, the concentration of thiobarbituric acid reactive substances (TBARS), the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx), and the activity of acid phosphatase (AcP), arylsulfatase (ASA), cathepsin D (CTSD), andα1-antitrypsin (AAT) were assayed. No statistically significant differences in investigated parameters were found between studied groups. Moreover, TBARS concentration and CAT, SOD, and GPx activity were not altered in the course of both types of surgery. Five days after both open and laparoscopic nephrectomy techniques, AAT activity was higher than its activity 12 hours after the procedure. The obtained results suggest that laparoscopy may be used for nephrectomy as effectively as open surgery without creating greater oxidative stress. Reduced period of convalescence at patients treated with laparoscopy may be due to less severe response of acute-phase proteins.

scholarly journals HIF2α Exerts Carcinogenesis in Clear Cell Renal Cell Carcinoma by Targeting High Expression of NUDT1 to Inhibit Oxidative Stress

2020 ◽  
Author(s):  
Jian Shi ◽  
Zhiyong Xiong ◽  
Keshan Wang ◽  
Changfei Yuan ◽  
Yu Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Clear Cell ◽  
Experimental Studies ◽  
Luciferase Reporter ◽  
Sequencing Data ◽  
Cell Renal Cell Carcinoma ◽  
And Oxidative Stress

Abstract Background: Emerging evidence highlights the important roles of HIF2α in the development and progression of clear cell renal cell carcinoma (ccRCC). Recently, oxidative stress has been shown to play a vital role in an increasing number of tumor types. However, the relationship between the two factors in ccRCC is still unclear. The aim of this work is to study the role of oxidative stress in ccRCC and its relationship with HIF2α.Methods: Molecular screening and bioinformatics analysis in ccRCC based on data from the TCGA database. Regulated pathways were investigated by qRT-PCR, immunoblot analysis, luciferase reporter and chromatin immunoprecipitation (ChIP) assays. A series of functional analyses were conducted in cell lines and xenograft models.Results: By screening three independent oxidative stress-related gene sets and the whole transcriptome sequencing data obtained after HIF2α knockdown, NUDT1 was discovered as a bridge molecule mediating the interrelationship between HIF2α and oxidative stress. Bioinformatics and experimental studies have found that NUDT1 is upregulated in ccRCC and has significant prognostic implications. Mechanistically, HIF2α directly increases NUDT1 expression by binding to HIF2α response elements in the NUDT1 promoter region. Reducing the expression of NUDT1 can significantly increase the level of oxidative stress in ccRCC cells, resulting in the inhibition of the carcinogenic effect of HIF2α.Conclusions: Our research systematically identify the regulatory mechanisms of HIF2α and oxidative stress in ccRCC for the first time. It provides a new understanding of ccRCC and can help us creating new strategies for its treatment.

Misoprostol modulates the gene expression prostaglandin E2 and oxidative stress markers in myometrial cells

2016 ◽  
Vol 126 ◽  
pp. 38-45
Author(s):  
Cristine Kolling Konopka ◽  
Verônica Farina Azzolin ◽  
Francine Carla Cadoná ◽  
Alencar Kolinski Machado ◽  
Eduardo Bortoluzzi Dornelles ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Prostaglandin E2 ◽  
Oxidative Stress Markers ◽  
Myometrial Cells ◽  
Stress Markers ◽  
And Oxidative Stress

Comparison of Salivary Antioxidants and Oxidative Stress Status in Gestational Diabetes Mellitus and Healthy Pregnant Women

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Shima Fathi ◽  
Mohammad Taghi Goodarzi ◽  
Shiva Borzouei ◽  
Jalal Poorolajal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Oxidative Stress Markers ◽  
Blood Samples ◽  
Stress Markers ◽  
Total Antioxidant ◽  
Total Oxidative Stress ◽  
And Oxidative Stress

Background: One of the most common complications of pregnant women is gestational diabetes mellitus (GDM). Oxidative stress can play an important role in GDM. Objective: The aim of this study was to evaluate salivary antioxidants and oxidative stress markers in GDM. Method: Twenty pregnant women with GDM and 20 healthy pregnant women with normal blood glucose test participated in this study. Five mL of unstimulated saliva samples were collected. Spectrophotometric assay was carried out for sialochemical analysis. Stata software was used for data analysis. Results: The GDM group exhibited no significant difference in salivary total antioxidant capacity and malondialdehyde compared to the healthy control group. All of antioxidants markers, the uric acid, total antioxidant, peroxidase and catalase, decreased in GDM group that the difference of peroxidase and catalase was statistically significant. All of oxidative stress markers, the salivary malondyaldehid, total oxidative stress and total thiol, increased in GDM group. GDM group exhibited significantly higher salivary total oxidative stress levels. Conclusion: Catalase level was significantly lower and total oxidative stress was significantly higher. These two markers might have significant importance and might exhibit early changes compared to other factors in GDM. . Some of salivary antioxidants might have diagnostic, prognostic or therapeutic implications in GDM. Other studies with large sample size on salivary and blood samples need to be done to confirm this properties and salivary samples using instead of blood samples in GDM biomarkers changes.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Type 2 Diabetes Mellitus: A Retrospective Cohort Study

2020 ◽  
Vol 20 (4) ◽  
pp. 584-590 ◽  
Author(s):  
Shima Fathi ◽  
Shiva Borzouei ◽  
Mohammad Taghi Goodarzi ◽  
Jalal Poorolajal ◽  
Fatemeh Ahmadi-Motamayel
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Type 2 Dm ◽  
Patients With Diabetes ◽  
The Difference ◽  
And Oxidative Stress

Background: Diabetes Mellitus (DM) is a progressive metabolic disorder. Objective: The aim of this study was to investigate the relationship between antioxidant and oxidative stress markers in the saliva of patients with type 2 DM and a healthy control group. Methods: In this study, 20 patients with diabetes and 20 healthy individuals were evaluated. Salivary antioxidants markers consisted of total antioxidant capacity (TAC), uric acid (UA), peroxidase and catalase. Oxidative stress markers included total oxidant status (TOS), malondealdehyde (MDA) and total thiol (SH). Sialochemical analysis was performed with spectrophotometric assay. All the statistical analyses were conducted using STATA software. Results: TAC decreased significantly in patients with diabetes. Although salivary UA and peroxidase were lower in patients with diabetes compared to the control group, the difference was not significant. Salivary catalase in patients with diabetes was significantly lower than that in the control group. MDA and TOS exhibited significantly higher levels in type 2 DM. SH levels were slightly higher in DM. Conclusions: According to the results of the present study, there were some changes in the salivary levels of some antioxidants and oxidative stress markers in patients with type 2 DM and could be measured as an indicator of serum changes..

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