Inhibition of replication of classical swine fever virus in a stable cell line by the viral capsid and Staphylococcus aureus nuclease fusion protein

2010 ◽  
Vol 167 (1) ◽  
pp. 79-83 ◽  
Author(s):  
Bin Zhou ◽  
Ke Liu ◽  
Jian-Chao Wei ◽  
Xiang Mao ◽  
Pu-yan Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Fusion Protein ◽  
Cell Line ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Stable Cell Line ◽  
Viral Capsid ◽  
And Staphylococcus Aureus

scholarly journals Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus

2020 ◽  
Vol 64 (1) ◽  
pp. 9-14
Author(s):  
Mei Yin ◽  
Dongfang Hu ◽  
Peng Li ◽  
Lingyun Kong ◽  
Hongmei Ning ◽  
...  
Keyword(s):  
Cell Line ◽  
Classical Swine Fever Virus ◽  
Vaccine Development ◽  
Cell Clone ◽  
Classical Swine Fever ◽  
High Titre ◽  
Fever Virus ◽  
Economic Losses ◽  
Cell Clones

AbstractIntroductionClassical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection.Material and MethodsTo achieve a homogeneous PK15 cell line which enabled high titre replication of CSFV, we used the limiting dilution cell cloning method.ResultsWe developed two cell clones, PK15-1A6 and PK15-3B1, which respectively have high- and low-permissive phenotypes to CSFV infection. The PK15-1A6, PK15-3B1, and PK15 parent cells showed different characteristics in cell proliferation rate, susceptibility to CSFV infection, and CSFV production. The mean virus titres per millilitre reflected by TCID50 values in PK15-1A6, PK15-3B1, and PK15 parent cells were 106.85, 103.63, and 104.74, respectively.ConclusionThe PK15-1A6 cell clone is more permissive to CSFV infection than the PK15 parent cells. The screened high-permissive cells will be useful for CSFV propagation and vaccine development in vitro, and facilitate research on the pathogenicity of CSFV.

scholarly journals In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line

2004 ◽  
Vol 35 (6) ◽  
pp. 625-633 ◽  
Author(s):  
Emilia Campos ◽  
Concepci�n Revilla ◽  
Sonia Chamorro ◽  
Bel�n Alvarez ◽  
Angel Ezquerra ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Porcine Aortic Endothelial Cell ◽  
Aortic Endothelial Cell ◽  
Endothelial Cell Line ◽  
Vitro Effect

scholarly journals Classical Swine Fever Virus ErnsDeletion Mutants: trans-Complementation and Potential Use as Nontransmissible, Modified, Live-Attenuated Marker Vaccines

2000 ◽  
Vol 74 (7) ◽  
pp. 2973-2980 ◽  
Author(s):  
M. N. Widjojoatmodjo ◽  
H. G. P. van Gennip ◽  
A. Bouma ◽  
P. A. van Rijn ◽  
R. J. M. Moormann
Keyword(s):  
Amino Acids ◽  
Cell Line ◽  
Classical Swine Fever Virus ◽  
Lethal Dose ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Wild Type Virus ◽  
Deletion Mutants ◽  
Structural Protein ◽  
Viral Particles

ABSTRACT An SK6 cell line (SK6c26) which constitutively expressed the glycoprotein Erns of classical swine fever virus (CSFV) was used to rescue CSFV Erns deletion mutants based on the infectious copy of CSFV strain C. The biochemical properties of Erns from this cell line were indistinguishable from those of CSFV Erns. Two Erns deletion mutants were constructed, virus Flc23 and virus Flc22. Virus Flc23 encoded only the utmost N- and C-terminal amino acids of Erns (deletion of 215 amino acids) to retain the original protease cleavage sites. Virus Flc22 is not recognized by a panel of Erns antibodies, due to a deletion of 66 amino acids in Erns. The Erns deletion mutants Flc22 and Flc23 could be rescued in vitro only on the complementing SK6c26 cells. These rescued viruses could infect and replicate in SK6 cells but did not yield infectious virus. Virus neutralization by Erns-specific antibodies was similar for the wild-type virus and the recombinant viruses, indicating that Erns from SK6c26 cells was incorporated in the viral particles. Pigs vaccinated with Flc22 or Flc23 were protected against a challenge with a lethal dose of CSFV strain Brescia. This is the first demonstration of trans-complementation of defective pestivirus RNA with a pestiviral structural protein and opens new ways to develop nontransmissible modified live pestivirus vaccines. In addition, the absence of (the antigenic part of) Erns in the recombinant viral particles can be used to differentiate between infected and vaccinated animals.

scholarly journals A classical swine fever virus E2 fusion protein produced in plants elicits a neutralizing humoral immune response in mice and pigs

2020 ◽  
Vol 42 (7) ◽  
pp. 1247-1261 ◽  
Author(s):  
Youngmin Park ◽  
Sangmin Lee ◽  
Hyangju Kang ◽  
Minhee Park ◽  
Kyungmin Min ◽  
...  
Keyword(s):  
Immune Response ◽  
Fusion Protein ◽  
Classical Swine Fever Virus ◽  
Humoral Immune Response ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Humoral Immune
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