scholarly journals Detection of oseltamivir sensitive/resistant strains of pandemic influenza A virus (H1N1) from patients admitted to hospitals in Thailand

2011 ◽  
Vol 177 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Sunchai Payungporn ◽  
Wittaya Poomipak ◽  
Jarika Makkoch ◽  
Pornpimol Rianthavorn ◽  
Apiradee Theamboonlers ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Virus H1n1 ◽  
Resistant Strains

scholarly journals One-step real-time RT-PCR for pandemic influenza A virus (H1N1) 2009 matrix gene detection in swine samples

2010 ◽  
Vol 164 (1-2) ◽  
pp. 83-87 ◽  
Author(s):  
Alessio Lorusso ◽  
Kay S. Faaberg ◽  
Mary Lea Killian ◽  
Leo Koster ◽  
Amy L. Vincent
Keyword(s):  
Real Time ◽  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Virus H1n1 ◽  
Rt Pcr ◽  
Gene Detection ◽  
Matrix Gene ◽  
One Step

scholarly journals Reassortants of pandemic influenza A virus H1N1/2009 and endemic porcine HxN2 viruses emerge in swine populations in Germany

2012 ◽  
Vol 93 (8) ◽  
pp. 1658-1663 ◽  
Author(s):  
Elke Starick ◽  
Elke Lange ◽  
Christian Grund ◽  
Elisabeth grosse Beilage ◽  
Stefanie Döhring ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Swine Influenza ◽  
Biological Properties ◽  
Influenza Viruses ◽  
West Germany ◽  
Virus H1n1 ◽  
North West ◽  
Synonymous Mutations

The incursion of the human pandemic influenza A virus H1N1 (2009) (H1N1 pdm) into pig populations and its ongoing co-circulation with endemic swine influenza viruses (SIVs) has yielded distinct human–porcine reassortant virus lineages. The haemagglutinin (HA) gene of H1N1 pdm was detected in 41 influenza virus-positive samples from seven swine herds in north-west Germany in 2011. Eight of these samples yielded virus that carried SIV-derived neuraminidase N2 of three different porcine lineages in an H1N1 pdm backbone. The HA sequences of these viruses clustered in two distinct groups and were distinguishable from human and other porcine H1 pdm by a unique set of eight non-synonymous mutations. In contrast to the human population, where H1N1 pdm replaced seasonal H1N1, this virus seems to co-circulate and interact more intensely with endemic SIV lineages, giving rise to reassortants with as-yet-unknown biological properties and undetermined risks for public health.

Molecular characteristics of the human pandemic influenza A virus (H1N1)

2010 ◽  
Vol 54 (3) ◽  
pp. 155-163 ◽  
Author(s):  
S. PAYUNGPORN ◽  
N. PANJAWORAYAN ◽  
J. MAKKOCH ◽  
Y. POOVORAWAN
Keyword(s):  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Molecular Characteristics ◽  
Virus H1n1

scholarly journals Effect of Clinical and Virological Parameters on the Level of Neutralizing Antibody against Pandemic Influenza A Virus H1N1 2009

2010 ◽  
Vol 51 (3) ◽  
pp. 274-279 ◽  
Author(s):  
Ivan F. N. Hung ◽  
Kelvin K. W. To ◽  
Cheuk‐Kwong Lee ◽  
Chi‐Kit Lin ◽  
Jasper F. W. Chan ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Neutralizing Antibody ◽  
Virus H1n1

scholarly journals Altered Viral Replication and Cell Responses by Inserting MicroRNA Recognition Element into PB1 in Pandemic Influenza A Virus (H1N1) 2009

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Xiaoyue Shen ◽  
Wenkui Sun ◽  
Yi Shi ◽  
Zheng Xing ◽  
Xin Su
Keyword(s):  
Epithelial Cells ◽  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Bronchial Epithelial Cells ◽  
Virus H1n1 ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Recognition Element ◽  
A Cell

Objective. MicroRNAs (miRNAs) are endogenous noncoding RNAs that spatiotemporally modulate mRNAs in a posttranscriptional manner. Engineering mutant viruses by inserting cell-specific miRNA recognition element (MRE) into viral genome may alter viral infectivity and host responses in vital tissues and organs infected with pandemic influenza A virus (H1N1) 2009 (H1N1pdm).Methods. In this study, we employed reverse genetics approach to generate a recombinant H1N1pdm with a cell-specific miRNA target sequence inserted into its PB1 genomic segment to investigate whether miRNAs are able to suppress H1N1pdm replication. We inserted an MRE of microRNA-let-7b (miR-let-7b) into the open reading frame of PB1 to test the feasibility of creating a cell-restricted H1N1pdm virus since let-7b is abundant in human bronchial epithelial cells.Results. miR-let-7b is rich in human bronchial epithelial cells (HBE). Incorporation of the miR-let-7b-MRE confers upon the recombinant H1N1pdm virus susceptibility to miR-let-7b targeting, suggesting that the H1N1pdm and influenza A viruses can be engineered to exert the desired replication restrictive effect and decrease infectivity in vital tissues and organs.Conclusions. This approach provides an additional layer of biosafety and thus has great potential for the application in the rational development of safer and more effective influenza viral vaccines.

scholarly journals Development of Two Types of Rapid Diagnostic Test Kits To Detect the Hemagglutinin or Nucleoprotein of the Swine-Origin Pandemic Influenza A Virus H1N1

2011 ◽  
Vol 18 (3) ◽  
pp. 494-499 ◽  
Author(s):  
Rika Mizuike ◽  
Tadahiro Sasaki ◽  
Koichi Baba ◽  
Hisahiko Iwamoto ◽  
Yusuke Shibai ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Seasonal Influenza ◽  
Virus Detection ◽  
Virus H1n1 ◽  
Viral Hemagglutinin ◽  
Detection Assay ◽  
Influenza A H1n1 ◽  
New Type

ABSTRACTSince its emergence in April 2009, pandemic influenza A virus H1N1 (H1N1 pdm), a new type of influenza A virus with a triple-reassortant genome, has spread throughout the world. Initial attempts to diagnose the infection in patients using immunochromatography (IC) relied on test kits developed for seasonal influenza A and B viruses, many of which proved significantly less sensitive to H1N1 pdm. Here, we prepared monoclonal antibodies that react with H1N1 pdm but not seasonal influenza A (H1N1 and H3N2) or B viruses. Using two of these antibodies, one recognizing viral hemagglutinin (HA) and the other recognizing nucleoprotein (NP), we developed kits for the specific detection of H1N1 pdm and tested them using clinical specimens of nasal wash fluid or nasopharyngeal fluid from patients with influenza-like illnesses. The specificities of both IC test kits were very high (93% for the HA kit, 100% for the NP kit). The test sensitivities for detection of H1N1 pdm were 85.5% with the anti-NP antibody, 49.4% with the anti-HA antibody, and 79.5% with a commercially available influenza A virus detection assay. Use of the anti-NP antibody could allow the rapid and accurate diagnosis of H1N1 pdm infections.

Investigation of an Outbreak of 2009 Pandemic Influenza A Virus (H1N1) Infections among Healthcare Personnel in a Chicago Hospital

2011 ◽  
Vol 32 (6) ◽  
pp. 611-615 ◽  
Author(s):  
Shelley S. Magill ◽  
Stephanie R. Black ◽  
Matthew E. Wise ◽  
Alexander J. Kallen ◽  
Soo-Jeong Lee ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Healthcare Personnel ◽  
Pandemic H1n1 ◽  
Virus H1n1 ◽  
Hospital Outbreak ◽  
Healthcare Associated

In May 2009, we investigated a hospital outbreak of pandemic H1N1 (pH1N1) infection among healthcare personnel (HCP). Thirteen (65%) of 20 HCP with pH1N1 infection had healthcare-associated cases, which were primarily attributed to transmission among HCP. Eleven (55%) of HCP with pH1N1 infection worked for 1 day or more after the onset of illness. Personnel working with mild illness may have contributed to transmission among HCP.

scholarly journals Attaching Zanamivir to a Polymer Markedly Enhances Its Activity Against Drug-resistant Strains of Influenza a Virus

2011 ◽  
Vol 100 (3) ◽  
pp. 831-835 ◽  
Author(s):  
Alisha K. Weight ◽  
Jayanta Haldar ◽  
Luis Álvarez de Cienfuegos ◽  
Larisa V. Gubareva ◽  
Terrence M. Tumpey ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Drug Resistant ◽  
Resistant Strains ◽  
Drug Resistant Strains
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