scholarly journals Development of Two Types of Rapid Diagnostic Test Kits To Detect the Hemagglutinin or Nucleoprotein of the Swine-Origin Pandemic Influenza A Virus H1N1

2011 ◽  
Vol 18 (3) ◽  
pp. 494-499 ◽  
Author(s):  
Rika Mizuike ◽  
Tadahiro Sasaki ◽  
Koichi Baba ◽  
Hisahiko Iwamoto ◽  
Yusuke Shibai ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Seasonal Influenza ◽  
Virus Detection ◽  
Virus H1n1 ◽  
Viral Hemagglutinin ◽  
Detection Assay ◽  
Influenza A H1n1 ◽  
New Type

ABSTRACTSince its emergence in April 2009, pandemic influenza A virus H1N1 (H1N1 pdm), a new type of influenza A virus with a triple-reassortant genome, has spread throughout the world. Initial attempts to diagnose the infection in patients using immunochromatography (IC) relied on test kits developed for seasonal influenza A and B viruses, many of which proved significantly less sensitive to H1N1 pdm. Here, we prepared monoclonal antibodies that react with H1N1 pdm but not seasonal influenza A (H1N1 and H3N2) or B viruses. Using two of these antibodies, one recognizing viral hemagglutinin (HA) and the other recognizing nucleoprotein (NP), we developed kits for the specific detection of H1N1 pdm and tested them using clinical specimens of nasal wash fluid or nasopharyngeal fluid from patients with influenza-like illnesses. The specificities of both IC test kits were very high (93% for the HA kit, 100% for the NP kit). The test sensitivities for detection of H1N1 pdm were 85.5% with the anti-NP antibody, 49.4% with the anti-HA antibody, and 79.5% with a commercially available influenza A virus detection assay. Use of the anti-NP antibody could allow the rapid and accurate diagnosis of H1N1 pdm infections.

Viral load and epidemiological profile of patients infected by pandemic influenza a (H1N1) 2009 and seasonal influenza a virus in Southern Brazil

2012 ◽  
Vol 84 (3) ◽  
pp. 371-379 ◽  
Author(s):  
Ana Beatriz Gorini da Veiga ◽  
Nélson Alexandre Kretzmann ◽  
Laura Trevizan Corrêa ◽  
Alessandra Mari Goshiyama ◽  
Tatiana Baccin ◽  
...  
Keyword(s):  
Viral Load ◽  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Seasonal Influenza ◽  
Southern Brazil ◽  
Influenza A H1n1 ◽  
Epidemiological Profile

A structure-free digital microfluidic platform for detection of influenza a virus by using magnetic beads and electromagnetic forces

Lab on a Chip ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 789-797 ◽  
Author(s):  
Po-Hsien Lu ◽  
Yu-Dong Ma ◽  
Chien-Yu Fu ◽  
Gwo-Bin Lee
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Magnetic Beads ◽  
H1n1 Virus ◽  
Virus Detection ◽  
Electromagnetic Forces ◽  
Microfluidic Platform ◽  
Influenza A H1n1 ◽  
New Type ◽  
Digital Microfluidic

A new type of digital microfluidic platform for influenza A H1N1 virus detection by utilizing a one-aptamer/two-antibodies assay on magnetic beads was reported.

scholarly journals Hospitalized Children with 2009 Pandemic Influenza A (H1N1): Comparison to Seasonal Influenza and Risk Factors for Admission to the ICU

PLoS ONE ◽  
2010 ◽  
Vol 5 (12) ◽  
pp. e15173 ◽  
Author(s):  
Dayanand Bagdure ◽  
Donna J. Curtis ◽  
Emily Dobyns ◽  
Mary P. Glodé ◽  
Samuel R. Dominguez
Keyword(s):  
Risk Factors ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Seasonal Influenza ◽  
Hospitalized Children ◽  
Influenza A H1n1

scholarly journals Contemporary Seasonal Influenza A (H1N1) Virus Infection Primes for a More Robust Response To Split Inactivated Pandemic Influenza A (H1N1) Virus Vaccination in Ferrets

2010 ◽  
Vol 17 (12) ◽  
pp. 1998-2006 ◽  
Author(s):  
Ali H. Ellebedy ◽  
Thomas P. Fabrizio ◽  
Ghazi Kayali ◽  
Thomas H. Oguin ◽  
Scott A. Brown ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Seasonal Influenza ◽  
H1n1 Virus ◽  
H1n1 Influenza ◽  
Influenza Viruses ◽  
Inactivated Vaccine ◽  
H1n1 Influenza Virus ◽  
Influenza A H1n1

ABSTRACT Human influenza pandemics occur when influenza viruses to which the population has little or no immunity emerge and acquire the ability to achieve human-to-human transmission. In April 2009, cases of a novel H1N1 influenza virus in children in the southwestern United States were reported. It was retrospectively shown that these cases represented the spread of this virus from an ongoing outbreak in Mexico. The emergence of the pandemic led to a number of national vaccination programs. Surprisingly, early human clinical trial data have shown that a single dose of nonadjuvanted pandemic influenza A (H1N1) 2009 monovalent inactivated vaccine (pMIV) has led to a seroprotective response in a majority of individuals, despite earlier studies showing a lack of cross-reactivity between seasonal and pandemic H1N1 viruses. Here we show that previous exposure to a contemporary seasonal H1N1 influenza virus and to a lesser degree a seasonal influenza virus trivalent inactivated vaccine is able to prime for a higher antibody response after a subsequent dose of pMIV in ferrets. The more protective response was partially dependent on the presence of CD8+ cells. Two doses of pMIV were also able to induce a detectable antibody response that provided protection from subsequent challenge. These data show that previous infection with seasonal H1N1 influenza viruses likely explains the requirement for only a single dose of pMIV in adults and that vaccination campaigns with the current pandemic influenza vaccines should reduce viral burden and disease severity in humans.

scholarly journals Immunogenicity, Boostability, and Sustainability of the Immune Response after Vaccination against Influenza A Virus (H1N1) 2009 in a Healthy Population

2011 ◽  
Vol 18 (9) ◽  
pp. 1401-1405 ◽  
Author(s):  
Elisabeth Huijskens ◽  
John Rossen ◽  
Paul Mulder ◽  
Ruud van Beek ◽  
Hennie van Vugt ◽  
...  
Keyword(s):  
Immune Response ◽  
Influenza Virus ◽  
Influenza A Virus ◽  
Influenza A ◽  
Seasonal Influenza ◽  
Virus H1n1 ◽  
Prospective Longitudinal Study ◽  
Virus Vaccination ◽  
Short Period ◽  
Prospective Longitudinal

ABSTRACTThe emergence of a new influenza A virus (H1N1) variant in 2009 led to a worldwide vaccination program, which was prepared in a relatively short period of time. This study investigated the humoral immunity against this virus before and after vaccination with a 2009 influenza A virus (H1N1) monovalent MF59-adjuvanted vaccine, as well as the persistence of vaccine-induced antibodies. Our prospective longitudinal study included 498 health care workers (mean age, 43 years; median age, 44 years). Most (89%) had never or only occasionally received a seasonal influenza virus vaccine, and 11% were vaccinated annually (on average, for >10 years). Antibody titers were determined by a hemagglutination inhibition (HI) assay at baseline, 3 weeks after the first vaccination, and 5 weeks and 7 months after the second vaccination. Four hundred thirty-five persons received two doses of the 2009 vaccine. After the first dose, 79.5% developed a HI titer of ≥40. This percentage increased to 83.3% after the second dose. Persistent antibodies were found in 71.9% of the group that had not received annual vaccinations and in 43.8% of the group that had received annual vaccinations. The latter group tended to have lower HI titers (P=0.09). With increasing age, HI titers decreased significantly, by 2.4% per year. A single dose of the 2009 vaccine was immunogenic in almost 80% of the study population, whereas an additional dose resulted in significantly increased titers only in persons over 50. Finally, a reduced HI antibody response against the 2009 vaccine was found in adults who had previously received seasonal influenza virus vaccination. More studies on the effect of yearly seasonal influenza virus vaccination on the immune response are warranted.

scholarly journals Response to 2009 Pandemic Influenza A (H1N1) Vaccine in HIV-Infected Patients and the Influence of Prior Seasonal Influenza Vaccination

PLoS ONE ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. e16496 ◽  
Author(s):  
Darius Soonawala ◽  
Guus F. Rimmelzwaan ◽  
Luc B. S. Gelinck ◽  
Leo G. Visser ◽  
Frank P. Kroon
Keyword(s):  
Influenza Vaccination ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Seasonal Influenza ◽  
H1n1 Vaccine ◽  
Seasonal Influenza Vaccination ◽  
Influenza A H1n1

scholarly journals One-step real-time RT-PCR for pandemic influenza A virus (H1N1) 2009 matrix gene detection in swine samples

2010 ◽  
Vol 164 (1-2) ◽  
pp. 83-87 ◽  
Author(s):  
Alessio Lorusso ◽  
Kay S. Faaberg ◽  
Mary Lea Killian ◽  
Leo Koster ◽  
Amy L. Vincent
Keyword(s):  
Real Time ◽  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Virus H1n1 ◽  
Rt Pcr ◽  
Gene Detection ◽  
Matrix Gene ◽  
One Step

scholarly journals Reassortants of pandemic influenza A virus H1N1/2009 and endemic porcine HxN2 viruses emerge in swine populations in Germany

2012 ◽  
Vol 93 (8) ◽  
pp. 1658-1663 ◽  
Author(s):  
Elke Starick ◽  
Elke Lange ◽  
Christian Grund ◽  
Elisabeth grosse Beilage ◽  
Stefanie Döhring ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Swine Influenza ◽  
Biological Properties ◽  
Influenza Viruses ◽  
West Germany ◽  
Virus H1n1 ◽  
North West ◽  
Synonymous Mutations

The incursion of the human pandemic influenza A virus H1N1 (2009) (H1N1 pdm) into pig populations and its ongoing co-circulation with endemic swine influenza viruses (SIVs) has yielded distinct human–porcine reassortant virus lineages. The haemagglutinin (HA) gene of H1N1 pdm was detected in 41 influenza virus-positive samples from seven swine herds in north-west Germany in 2011. Eight of these samples yielded virus that carried SIV-derived neuraminidase N2 of three different porcine lineages in an H1N1 pdm backbone. The HA sequences of these viruses clustered in two distinct groups and were distinguishable from human and other porcine H1 pdm by a unique set of eight non-synonymous mutations. In contrast to the human population, where H1N1 pdm replaced seasonal H1N1, this virus seems to co-circulate and interact more intensely with endemic SIV lineages, giving rise to reassortants with as-yet-unknown biological properties and undetermined risks for public health.

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