Development and evaluation of a reverse transcription-insulated isothermal polymerase chain reaction (RT-iiPCR) assay for detection of equine arteritis virus in equine semen and tissue samples using the POCKIT™ system

2016 ◽  
Vol 234 ◽  
pp. 7-15 ◽  
Author(s):  
Mariano Carossino ◽  
Pei-Yu A. Lee ◽  
Bora Nam ◽  
Ashley Skillman ◽  
Kathleen M. Shuck ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Equine Arteritis Virus ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Polymerase Chain

scholarly journals Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

2000 ◽  
Vol 25 (2) ◽  
pp. 169-193 ◽  
Author(s):  
SA Bustin
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Housekeeping Gene ◽  
Absolute Quantification ◽  
Clear Understanding ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Quantitative Measurements ◽  
Polymerase Chain

The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples. However, it is a complex technique, there are substantial problems associated with its true sensitivity, reproducibility and specificity and, as a quantitative method, it suffers from the problems inherent in PCR. The recent introduction of fluorescence-based kinetic RT-PCR procedures significantly simplifies the process of producing reproducible quantification of mRNAs and promises to overcome these limitations. Nevertheless, their successful application depends on a clear understanding of the practical problems, and careful experimental design, application and validation remain essential for accurate quantitative measurements of transcription. This review discusses the technical aspects involved, contrasts conventional and kinetic RT-PCR methods for quantitating gene expression and compares the different kinetic RT-PCR systems. It illustrates the usefulness of these assays by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH).

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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