Nutrient enrichment of surface waters degrades water quality. Municipalities need effective and economical solutions to remove nutrients from surface waters. From July 2016 to May 2020, full-scale (900 m2, 5% cover) floating treatment wetlands (FTWs) were deployed in Wickham Park pond, a eutrophic water body (0.13 mg/L total phosphorus (P), 0.96 mg/L total nitrogen (N)). The plants in FTWs in close proximity to a SB10000 mixer fixed N and P more efficiently. The rate of N (g/m2/year) fixed within tissues was highest for Juncus effusus (13.5), Agrostis alba (13.2), and Sagittaria lancifolia (12.1). The rate of P (g/m2/year) fixed within plant tissues was similar for all species (3.77, Agrostis alba, Canna spp., Iris hexagona, Juncus effusus, and Sagittaria lancifolia) save Pontederia cordata (2.52) volunteer species (1.41). The N and P removed with plant harvest were similar for non-mixed and mixed FTWs. Notably, the N:P ratio in plant tissues in 2017 (pre-mixer installation) was 11:1; after mixer installation (2018–2020), N:P ratios averaged 2.7:1, indicating increased P fixation within plant tissues. In 2017, 12,828 kg of plant tissues was harvested, removing 334 kg of N and 29.5 kg of P. In 2019, 32,958 kg of plant biomass was harvested from the pond, removing 425 kg of N and 138 kg of P. In 2020, 27,945 kg of biomass was harvested from FTWs, removing 267 kg of N and 95 kg of P. From 2016 to 2020, 73,000 kg of biomass was harvested, removing 1026 kg of N and 262 kg of P from Wickham Park pond. Knowing the total fresh biomass of tissues removed from FTWs at harvest is critical for accuracy in reporting nutrient removal aided by FTWs.