Enrichment and domestication of a microbial consortium for degrading aniline

2021 ◽  
Vol 42 ◽  
pp. 102108
Author(s):  
Hui Chen ◽  
Chongran Sun ◽  
Ronghui Liu ◽  
Mingzhu Yuan ◽  
Zhenhua Mao ◽  
...  
Keyword(s):  
Microbial Consortium

Effect of Solids Retention Time on Nitrogen Removal and Microbial Consortium in a Novel Algal-Bacterial Shortcut Nitrogen Removal System

2017 ◽  
Vol 2017 (3) ◽  
pp. 225-230
Author(s):  
Nadezhda Zalivina ◽  
Ryan Keeley ◽  
Larissa T Arashiro ◽  
Angelica Rada-Ariza ◽  
Meng Wang ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Retention Time ◽  
Microbial Consortium ◽  
Solids Retention Time ◽  
Solids Retention ◽  
Shortcut Nitrogen Removal ◽  
Removal System

Production of 1,3-propanediol and lactic acid from crude glycerol by a microbial consortium from intertidal sludge

2021 ◽  
Author(s):  
Li-Li Jiang ◽  
Feng-Yi Liu ◽  
Wei Yang ◽  
Chang-Li Li ◽  
Bao-Wei Zhu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Crude Glycerol ◽  
Microbial Consortium

Efficient Lignin Decomposing Microbial Consortium to Hasten Rice-Straw Composting with Moderate GHGs Fluxes

2021 ◽  
Author(s):  
Pradeep Kumar Dash ◽  
Soumya Ranjan Padhy ◽  
Pratap Bhattacharyya ◽  
Ankita Pattanayak ◽  
Soumya Routray ◽  
...  
Keyword(s):  
Rice Straw ◽  
Microbial Consortium

Spatial Engineering of Microbial Consortium for Long‐Lasting, Self‐Sustaining, and High‐Power Generation in a Bacteria‐Powered Biobattery

2021 ◽  
pp. 2100713
Author(s):  
Lin Liu ◽  
Maedeh Mohammadifar ◽  
Anwar Elhadad ◽  
Mehdi Tahernia ◽  
Yunxiang Zhang ◽  
...  
Keyword(s):  
Power Generation ◽  
High Power ◽  
Microbial Consortium

scholarly journals Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Chaofeng Li ◽  
Xiaofeng Lin ◽  
Xing Ling ◽  
Shuo Li ◽  
Hao Fang
Keyword(s):  
Acid Production ◽  
Microbial Consortium ◽  
Consolidated Bioprocessing ◽  
High Titer ◽  
Value Added ◽  
Superior Performance ◽  
Glucaric Acid ◽  
Practical Applications ◽  
Work Distribution ◽  
Rut C30

Abstract Background The biomanufacturing of d-glucaric acid has attracted increasing interest because it is one of the top value-added chemicals produced from biomass. Saccharomyces cerevisiae is regarded as an excellent host for d-glucaric acid production. Results The opi1 gene was knocked out because of its negative regulation on myo-inositol synthesis, which is the limiting step of d-glucaric acid production by S. cerevisiae. We then constructed the biosynthesis pathway of d-glucaric acid in S. cerevisiae INVSc1 opi1Δ and obtained two engineered strains, LGA-1 and LGA-C, producing record-breaking titers of d-glucaric acid: 9.53 ± 0.46 g/L and 11.21 ± 0.63 g/L d-glucaric acid from 30 g/L glucose and 10.8 g/L myo-inositol in fed-batch fermentation mode, respectively. However, LGA-1 was preferable because of its genetic stability and its superior performance in practical applications. There have been no reports on d-glucaric acid production from lignocellulose. Therefore, the biorefinery processes, including separated hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP) were investigated and compared. CBP using an artificial microbial consortium composed of Trichoderma reesei (T. reesei) Rut-C30 and S. cerevisiae LGA-1 was found to have relatively high d-glucaric acid titers and yields after 7 d of fermentation, 0.54 ± 0.12 g/L d-glucaric acid from 15 g/L Avicel and 0.45 ± 0.06 g/L d-glucaric acid from 15 g/L steam-exploded corn stover (SECS), respectively. In an attempt to design the microbial consortium for more efficient CBP, the team consisting of T. reesei Rut-C30 and S. cerevisiae LGA-1 was found to be the best, with excellent work distribution and collaboration. Conclusions Two engineered S. cerevisiae strains, LGA-1 and LGA-C, with high titers of d-glucaric acid were obtained. This indicated that S. cerevisiae INVSc1 is an excellent host for d-glucaric acid production. Lignocellulose is a preferable substrate over myo-inositol. SHF, SSF, and CBP were studied, and CBP using an artificial microbial consortium of T. reesei Rut-C30 and S. cerevisiae LGA-1 was found to be promising because of its relatively high titer and yield. T. reesei Rut-C30 and S. cerevisiae LGA-1were proven to be the best teammates for CBP. Further work should be done to improve the efficiency of this microbial consortium for d-glucaric acid production from lignocellulose.

Role of Microbes and Microbial Consortium in Solid Waste Management

2021 ◽  
pp. 383-422
Author(s):  
Rachana Jain ◽  
Lopa Pattanaik ◽  
Susant Kumar Padhi ◽  
Satya Narayan Naik
Keyword(s):  
Waste Management ◽  
Solid Waste ◽  
Microbial Consortium ◽  
Solid Waste Management

scholarly journals Insight into the Metabolic Profiles of Pb(II) Removing Microorganisms

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4008
Author(s):  
Carla Cilliers ◽  
Evans M. N. Chirwa ◽  
Hendrik G. Brink
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Microbial Consortium ◽  
Luria Bertani ◽  
Nutrient Concentrations ◽  
Batch Experiments ◽  
Metabolic Study ◽  
Luria Bertani Broth ◽  
Tandem Mass Spectroscopy ◽  
Insight Into

The objective of the study was to gather insight into the metabolism of lead-removing microorganisms, coupled with Pb(II) removal, biomass viability and nitrate concentrations for Pb(II) bioremoval using an industrially obtained microbial consortium. The consortium used for study has proven to be highly effective at removing aqueous Pb(II) from solution. Anaerobic batch experiments were conducted with Luria-Bertani broth as rich growth medium over a period of 33 h, comparing a lower concentration of Pb(II) with a higher concentration at two different nutrient concentrations. Metabolite profiling and quantification were conducted with the aid of both liquid chromatography coupled with tandem mass spectroscopy (UPLC-HDMS) in a “non-targeted” fashion and high-performance liquid chromatography (HPLC) in a “targeted” fashion. Four main compounds were identified, and a metabolic study was conducted on each to establish their possible significance for Pb(II) bioremoval. The study investigates the first metabolic profile to date for Pb(II) bioremoval, which in turn can result in a clarified understanding for development on an industrial and microbial level.

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