The inhibitory effect of naringenin on atopic dermatitis induced by DNFB in NC/Nga mice

Atopic dermatitis (AD) is a chronic inflammatory skin disease caused mainly by immune dysregulation. This study explored the anti-inflammatory and immunomodulatory effects of the Centella asiatica ethanol extract (CA) on an AD-like dermal disorder. Treatment with CA inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in a dose-dependent manner in inflammatory stimulated HaCaT cells by interferon-γ (IFN-γ) and TNF-α-triggered inflammation. Eight-week-old BALB/c mice treated with 2,4-dinitrochlorobenzene (DNCB) were used as a mouse model of AD. In AD induce model, we had two types treatment of CA; skin local administration (80 µg/cm2, AD+CA-80) and oral administration (200 mg/kg/d, AD+CA-200). Interestingly, the CA-treated groups exhibited considerably decreased mast cell infiltration in the ear tissue. In addition, the expression of IL-6 in mast cells, as well as the expression of various pathogenic cytokines, such as TNF-α, IL-4, IL-5, IL-6, IL-10, IL-17, iNOS, COX-2, and CXCL9, was reduced in both AD+CA-80 and AD+CA-200 groups. Collectively, our data demonstrate the pharmacological role and signaling mechanism of CA in the regulation of allergic inflammation of the skin, which supports our hypothesis that CA could potentially be developed as a therapeutic agent for AD.

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Inhibitory effect of 5,6-dihydroergosteol-glucoside on atopic dermatitis-like skin lesions via suppression of NF-κB and STAT activation.

Journal of Dermatological Science ◽

10.1016/j.jdermsci.2015.06.005 ◽

2015 ◽

Vol 79 (3) ◽

pp. 252-261 ◽

Cited By ~ 13

Author(s):

Mira Jung ◽

Tae Hoon Lee ◽

Hyun Jeoung Oh ◽

Hakwon Kim ◽

Youngsook Son ◽

...

Keyword(s):

Atopic Dermatitis ◽

Skin Lesions ◽

Inhibitory Effect

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Inhibitory Effect ofNelumbo nucifera(Gaertn.) on the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/153568 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 20

Author(s):

Rajendra Karki ◽

Myung-A Jung ◽

Keuk-Jun Kim ◽

Dong-Wook Kim

Keyword(s):

Atopic Dermatitis ◽

Severity Score ◽

Histopathological Examination ◽

Skin Lesions ◽

Transepidermal Water Loss ◽

Inhibitory Effect ◽

Clinical Severity ◽

Inflammatory Skin Disease ◽

Level Measurement ◽

Different Doses

Atopic dermatitis (AD) is a chronic inflammatory skin disease which has a complex etiology that encompasses immunologic responses. The study was carried out to examine the effect ofNelumbo nucifera(Gaertn.) leaf (NL) on the AD-like skin lesion induced by repeated epicutaneous application of 2,4-dinitrochlorobenzene (DNCB) on the dorsal skin of NC/Nga mice. Three different doses of NL (5, 25, and 50 mg/mice/day) were administered orally from the day of sensitization with DNCB for 4 weeks. The efficacy of NL was judged by histopathological examination, blood IgE level, measurement of transepidermal water loss (TEWL), scratching behavior, and skin severity score. NL resulted in the suppression of clinical severity score, TEWL, scratching behavior, and blood IgE level. Histopathologic analyses revealed that thickening of the epidermis and mast cell degranulation was significantly reduced in NL group. These results suggest that NL may be a useful natural resource for the management of AD.

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Angelicae Dahuricae Radix Inhibits Dust Mite Extract-Induced Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/743075 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Hoyoung Lee ◽

Jun Kyoung Lee ◽

Hyekyung Ha ◽

Mee-Young Lee ◽

Chang-Seob Seo ◽

...

Keyword(s):

Atopic Dermatitis ◽

Skin Lesions ◽

Inhibitory Effect ◽

Raw 264.7 Cells ◽

No Production ◽

Raw 264.7 ◽

Mrna Levels ◽

Dependent Manner ◽

Hacat Cells ◽

Angelicae Dahuricae Radix

We examined whether Angelicae Dahuricae Radix (AR) suppresses the development of atopic dermatitis (AD)-like skin lesions induced byDermatophagoides farinaein NC/Nga mice. To investigate the effect of AR, we measured the AD severity score, measured plasma levels of IgE and histamine, and performed histological analysis in NC/Nga mice. We also confirmed the anti-inflammatory effects of AR by measuring TARC/CCL17 production from LPS-treated RAW 264.7 cells and mRNA levels of TARC and MDC/CCL22 in TNF-α/IFN-γ-treated HaCaT cells. 10 mg/day of AR extract was applied for 4 weeks to NC/Nga mice. Both the AR extract and 0.1% tacrolimus suppressed the development of AD-like skin lesions and reduced dermatitis scores of the back and ear skin. AR extracts caused an inhibition of histological changes induced by repeated application ofD. farinaeand a reduction of IgE and histamine levels in plasma (P<0.05). Furthermore, NO production in LPS-treated RAW 264.7 cells was diminished in a dose-dependent manner, and hTARC production and TARC and MDC mRNA levels in TNF-α/IFN-γ-treated HaCaT cells were diminished by AR. The inhibitory effect of AR on NO, TARC and MDC production may be associated with the suppression of AD-like skin lesions inD. farinae-induced NC/Nga mice.

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THE EVALUATION OF THE INFLUENCE OF CETRILEV (LEVOCETIRIZINE) ON THE LATE PHASE OF THE ATOPIC REACTION

Medical Science of Ukraine (MSU) ◽

10.32345/2664-4738.3-4.2018.14 ◽

2018 ◽

Vol 14 (3-4) ◽

pp. 103-107

Author(s):

D.V. Maltsev

Keyword(s):

Atopic Dermatitis ◽

Late Phase ◽

Inhibitory Effect ◽

Control Group ◽

Study Group ◽

Single Center ◽

Pronounced Increase ◽

Comparative Clinical Study ◽

Lymphocyte Activity ◽

Specific Allergen

Relevance. Previously demonstrated inhibitory effect of levocetirizine on the activity of eosinophils during the late phase of the atopic reaction, but the effect of the drug on the second component of the late phase – lymphocytes – is still not specified. Objective of the study – to evaluate the effect of levocetirizine (Cetrilev) therapy on the lymphocyte component of the late phase of atopic allergic reaction in humans. Materials and methods. A single-center, prospective, comparative clinical study was conducted. Patients of the study group (SG) (n = 31) with chronic atopic dermatitis and rhinitis received a 14-day therapy with levocetirizine, whereas the control group (CG) (n = 22) patients treated with desloratadine. On the 1st, 7th and 14th day, the inhibition of migration of lymphocytes reaction with mitogens and allergens was evaluated. Results. SG achieved a significant increase in the rate of inhibition of migration of lymphocytes with konkanavalin and fitohemeagglutinin mitogens and a more pronounced increase in inhibition of migration of lymphocytes with a specific allergen on the 14th day of therapy (p <0,05, Z <Z0,05), whereas in the CG, only a slight increase in the level of these indicators without achieving significant differences was observed (p> 0,05, Z> Z0,05). Conclusions. 14-day therapy with levocetirizine (Cetrilev) inhibits the activity of allergen-specific lymphocytes in children and adults with atopic rhinitis and dermatitis with normalization of the total lymphocyte activity, indicating the effect of this drug on the late phase of the atopic reaction.

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