scholarly journals Corrigendum to “The relationship between melanin production and lipofuscin formation in Tyrosinase gene knockout melanocytes using CRISPR/Cas9 system” [Life Sci. 284 (2021) 119915 (1–8)]

Life Sciences ◽  
2021 ◽  
pp. 120225
Author(s):  
Jae Ho Kim ◽  
Moon-Moo Kim
Keyword(s):  
Gene Knockout ◽  
Melanin Production ◽  
Tyrosinase Gene ◽  
System Life ◽  
The Relationship

scholarly journals What Are the Best Parents for Hybrid Progeny? An Investigation into the Human Pathogenic Fungus Cryptococcus

2021 ◽  
Vol 7 (4) ◽  
pp. 299
Author(s):  
Man You ◽  
Jianping Xu
Keyword(s):  
Pathogenic Fungus ◽  
Sequence Divergence ◽  
Hybrid Vigor ◽  
Phenotypic Trait ◽  
Hybrid Progeny ◽  
Melanin Production ◽  
Fluconazole Resistance ◽  
Human Pathogenic Fungus ◽  
Two Temperatures ◽  
The Relationship

Hybridization between more divergent organisms is likely to generate progeny with more novel genetic interactions and genetic variations. However, the relationship between parental genetic divergence and progeny phenotypic variation remains largely unknown. Here, using strains of the human pathogenic Cryptococcus, we investigated the patterns of such a relationship. Twenty-two strains with up to 15% sequence divergence were mated. Progeny were genotyped at 16 loci. Parental strains and their progeny were phenotyped for growth ability at two temperatures, melanin production at seven conditions, and susceptibility to the antifungal drug fluconazole. We observed three patterns of relationships between parents and progeny for each phenotypic trait, including (i) similar to one of the parents, (ii) intermediate between the parents, and (iii) outside the parental phenotypic range. We found that as genetic distance increases between parental strains, progeny showed increased fluconazole resistance and growth at 37 °C but decreased melanin production under various oxidative and nitrosative stresses. Our findings demonstrate that, depending on the traits, both evolutionarily more similar strains and more divergent strains may be better parents to generate progeny with hybrid vigor. Together, the results indicate the enormous potential of Cryptococcus hybrids in their evolution and adaptation to diverse conditions.

Potent Melanin Production Enhancement of Human Tyrosinase Gene by Tat and an Entrapment in Elastic Cationic Niosomes: Potential Application in Vitiligo Gene Therapy

2012 ◽  
Vol 80 (6) ◽  
pp. 953-960
Author(s):  
Jiradej Manosroi ◽  
Narinthorn Khositsuntiwong ◽  
Friedrich Götz ◽  
Rolf G. Werner ◽  
Worapaka Manosroi ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Potential Application ◽  
Melanin Production ◽  
Tyrosinase Gene ◽  
Cationic Niosomes ◽  
Human Tyrosinase

scholarly journals A vector for studying plasmid stability functions in Streptomyces

1988 ◽  
Vol 51 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Kevin Kendall ◽  
John Cullum
Keyword(s):  
Copy Number ◽  
Stability Region ◽  
Selectable Marker ◽  
Plasmid Stability ◽  
Stability Function ◽  
Melanin Production ◽  
Stability Functions ◽  
Tyrosinase Gene ◽  
Copy Number Plasmid ◽  
Low Copy Number

SummaryWe constructed a cloning vector (pMT603) based on the low copy number plasmid SCP2*. pMT6O3 is unstable because it lacks the SCP2* stability region and carries the selectable marker thiostrepton-resistance and a tyrosinase gene which results in melanin production. This allows easy testing of plasmid stability and we demonstrated its usefulness by cloning a plasmid stability function.

scholarly journals Fitness Landscape of the Fission Yeast Genome

2019 ◽  
Vol 36 (8) ◽  
pp. 1612-1623
Author(s):  
Leanne Grech ◽  
Daniel C Jeffares ◽  
Christoph Y Sadée ◽  
María Rodríguez-López ◽  
Danny A Bitton ◽  
...  
Keyword(s):  
Molecular Evolution ◽  
Comparative Genomics ◽  
Fitness Landscape ◽  
Gene Knockout ◽  
Transposon Mutagenesis ◽  
Yeast Genome ◽  
Noncoding Regions ◽  
Functional Regions ◽  
Transposon Insertions ◽  
The Relationship

Abstract The relationship between DNA sequence, biochemical function, and molecular evolution is relatively well-described for protein-coding regions of genomes, but far less clear in noncoding regions, particularly, in eukaryote genomes. In part, this is because we lack a complete description of the essential noncoding elements in a eukaryote genome. To contribute to this challenge, we used saturating transposon mutagenesis to interrogate the Schizosaccharomyces pombe genome. We generated 31 million transposon insertions, a theoretical coverage of 2.4 insertions per genomic site. We applied a five-state hidden Markov model (HMM) to distinguish insertion-depleted regions from insertion biases. Both raw insertion-density and HMM-defined fitness estimates showed significant quantitative relationships to gene knockout fitness, genetic diversity, divergence, and expected functional regions based on transcription and gene annotations. Through several analyses, we conclude that transposon insertions produced fitness effects in 66–90% of the genome, including substantial portions of the noncoding regions. Based on the HMM, we estimate that 10% of the insertion depleted sites in the genome showed no signal of conservation between species and were weakly transcribed, demonstrating limitations of comparative genomics and transcriptomics to detect functional units. In this species, 3′- and 5′-untranslated regions were the most prominent insertion-depleted regions that were not represented in measures of constraint from comparative genomics. We conclude that the combination of transposon mutagenesis, evolutionary, and biochemical data can provide new insights into the relationship between genome function and molecular evolution.

scholarly journals Construction and identification of Rab7-ROSA26 knock-in mice by CRISPR/Cas9 technology

2020 ◽  
Author(s):  
Qing Xu ◽  
Linfang Jin ◽  
Xiaowei Qi
Keyword(s):  
Genome Editing ◽  
Southern Blot ◽  
Renal Fibrosis ◽  
Gene Knockout ◽  
Gene Insertion ◽  
System A ◽  
A Genome ◽  
The Relationship

Abstract Background: CRISPR/Cas9 system, a Genome editing technique, can achieve the purpose of gene knockout and gene insertion. Because we want to study the relationship between Rab7 and renal fibrosis in vivo, we should construct Rab7-knock-in mice. Results: We constructed Rab7-knock-in mice by CRISPR/Cas9 technology. Using the identification of PCR, southern blot and sequencing, the detected results were in line with our expectation. Conclusions: We constructed Rab7-knock-in mice successful, which can be provided for studying the relationship between Rab7 and renal fibrosis.

Melanin Production in Escherichia coli from a Cloned Tyrosinase Gene

1990 ◽  
Vol 8 (7) ◽  
pp. 634-638 ◽  
Author(s):  
Guy della-Cioppa ◽  
Stephen J. Garger ◽  
Genadie G. Sverlow ◽  
Thomas H. Turpen ◽  
Laurence K. Grill
Keyword(s):  
Escherichia Coli ◽  
Melanin Production ◽  
Tyrosinase Gene

scholarly journals Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b: cloning, sequencing, and expression of the tyrosinase gene mepA.

1993 ◽  
Vol 175 (17) ◽  
pp. 5403-5410 ◽  
Author(s):  
J Mercado-Blanco ◽  
F García ◽  
M Fernández-López ◽  
J Olivares
Keyword(s):  
Rhizobium Meliloti ◽  
Melanin Production ◽  
Tyrosinase Gene ◽  
Sequencing And Expression

scholarly journals Lactobacillus helveticus-Fermented Milk Whey Suppresses Melanin Production by Inhibiting Tyrosinase through Decreasing MITF Expression

Nutrients ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 2082
Author(s):  
Nobutomo Ikarashi ◽  
Natsuko Fukuda ◽  
Makiba Ochiai ◽  
Mami Sasaki ◽  
Risako Kon ◽  
...  
Keyword(s):  
Skin Barrier ◽  
Fermented Milk ◽  
Tyrosinase Activity ◽  
Lactobacillus Helveticus ◽  
Skin Barrier Function ◽  
Mrna Levels ◽  
Melanin Production ◽  
Milk Whey ◽  
Tyrosinase Gene ◽  
Mitf Expression

Whey obtained from milk fermented by the Lactobacillus helveticus CM4 strain (LHMW) has been shown to improve skin barrier function and increase skin-moisturizing factors. In this study, we investigated the effects of LHMW on melanin production to explore the additional impacts of LHMW on the skin. We treated mouse B16 melanoma cells with α-melanocyte-stimulating hormone (α-MSH) alone or simultaneously with LHMW and measured the amount of melanin. The amount of melanin in B16 cells treated with α-MSH significantly increased by 2-fold compared with that in control cells, and tyrosinase activity was also elevated. Moreover, treatment with LHMW significantly suppressed the increase in melanin content and elevation of tyrosinase activity due to α-MSH. LHMW also suppressed the α-MSH-induced increased expression of tyrosinase, tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) at the protein and mRNA levels. Furthermore, the mRNA and protein microphthalmia-associated transcription factor (MITF) expression levels were significantly increased with treatment with α-MSH alone, which were also suppressed by LHMW addition. LHMW suppression of melanin production is suggested to involve inhibition of the expression of the tyrosinase gene family by lowering the MITF expression level. LHMW may have promise as a material for cosmetics with expected clinical application in humans.

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