Icariin improves pig sperm quality through antioxidant and antibacterial effects during liquid storage at 17°C

2022 ◽  
pp. 104827
Author(s):  
Zhongyun Kou ◽  
Chenyang Wang ◽  
Lei Gao ◽  
Guiyan Chu ◽  
Gongshe Yang ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Antibacterial Effects ◽  
Liquid Storage

Effects of iodine methionine on boar sperm quality during liquid storage at 17°C

2017 ◽  
Vol 52 (6) ◽  
pp. 1061-1066 ◽  
Author(s):  
Q Fang ◽  
J Wang ◽  
YY Hao ◽  
H Li ◽  
JX Hu ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Liquid Storage ◽  
Boar Sperm

Absence of seminal plasma from sperm-rich fraction decreases boar sperm quality characteristics during the course of liquid storage

2018 ◽  
Vol 198 ◽  
pp. 20-26 ◽  
Author(s):  
D.F. Leal ◽  
M.A. Torres ◽  
G.M. Ravagnani ◽  
S.M.M.K. Martins ◽  
F.V. Meirelles ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Quality Characteristics ◽  
Liquid Storage ◽  
Boar Sperm

scholarly journals Implication of Polyhistidine, a Novel Apoptosis Inhibitor, in Inhibiting Lipopolysaccharide-Induced Apoptosis in Boar Sperm

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 719 ◽  
Author(s):  
Tianzeng Song ◽  
Yi Shi ◽  
Yangang Wang ◽  
Izhar Hyder Qazi ◽  
Christiana Angel ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Storage Conditions ◽  
Potential Implication ◽  
Liquid Storage ◽  
Boar Sperm ◽  
Tlr4 Agonist ◽  
Reasonable Evidence ◽  
Boar Semen ◽  
Induced Apoptosis ◽  
Apoptosis Inhibitor

Lipopolysaccharide (LPS) released from Gram-negative bacteria binds to toll-like receptor 4 (TLR4) and induces boar sperm apoptosis. Similarly, polyhistidine (pHis), a TLR4 agonist, can also bind to TLR4. We hypothesized that pHis could inhibit LPS-induced sperm apoptosis by competitively binding to TLR4 to then improve sperm quality. Therefore, the objective of this study was to examine whether pHis can inhibit LPS-induced sperm apoptosis and affect sperm quality. The results showed that the concentrations of bacterial colonies were significantly increased from 36 to 120 h under liquid storage conditions (p < 0.05); however, concentrations of LPS in boar semen showed a relatively constant trend (4.98 ± 1.55 EU/mL) following 120 h storage. The addition of 100 μg/mL pHis in the BTS extender significantly improved boar sperm motility and viability at 37 °C, and it significantly (p < 0.05) inhibited boar sperm apoptosis under liquid storage (17 °C) and at 37 °C incubation conditions. The co-treatment of LPS and pHis further confirmed that pHis played its role in inhibiting LPS-induced sperm apoptosis. In conclusion, our preliminary findings provide reasonable evidence that pHis could act as an inhibitor of LPS-induced apoptosis in boar sperm stored for longer periods of time. pHis might inhibit LPS-induced sperm apoptosis by competitively binding to TLR4. Nevertheless, further mechanistic studies are awaited to fully elucidate its potential implication in inhibiting LSP-induced apoptosis.

Effects of Isatis root polysaccharide on boar sperm quality during liquid storage and in vitro fertilization

2019 ◽  
Vol 210 ◽  
pp. 106178 ◽  
Author(s):  
Zhiqiang Ren ◽  
Weike Shaoyong ◽  
Qian Li ◽  
Lu Ma ◽  
Junying Xiao ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Quality ◽  
Vitro Fertilization ◽  
Liquid Storage ◽  
Boar Sperm

Development of sperm sexing and associated assisted reproductive technology for sex preselection of captive bottlenose dolphins (Tursiops truncatus)

2006 ◽  
Vol 18 (3) ◽  
pp. 319 ◽  
Author(s):  
J. K. O'Brien ◽  
T. R. Robeck
Keyword(s):  
Tursiops Truncatus ◽  
Sperm Quality ◽  
Bottlenose Dolphins ◽  
Sperm Concentration ◽  
Motility Index ◽  
Liquid Storage ◽  
Directional Freezing

Research was conducted to develop sperm sorting and novel sperm preservation methodologies for sex predetermination in the bottlenose dolphin (Tursiops truncatus) using artificial insemination. In Study 1, the effect of seminal plasma (SP), sperm concentration and freezing rate (FR) on in vitro sperm quality of liquid-stored, non-sorted spermatozoa was examined. There was no effect (P > 0.05) of prefreeze SP addition on post-thaw quality (progressive motility, kinetic rating, sperm motility index (SMI), viability and acrosome integrity). Post-thaw motility parameters and viability were higher (P < 0.05) for slow FR than fast FR samples. In Study 2 investigating the effects of liquid storage and sorting on sperm quality, motility and SMI after sorting and centrifugation were lower (P < 0.05) than those of the initial ejaculate. The sort rate for enrichment (91 ± 4% purity) of X- and Y-bearing spermatozoa was 3400 ± 850 spermatozoa sex−1 s−1. In Study 3, compared with a modified straw method, directional freezing resulted in enhanced in vitro quality of sorted and non-sorted spermatozoa derived from liquid-stored semen (P < 0.05). In Study 4, endoscopic insemination of three dolphins with sorted, frozen–thawed X-bearing spermatozoa resulted in one conception and the birth of a female calf. High-purity sorting of dolphin spermatozoa, derived from liquid-stored semen, can be achieved with minimal loss of in vitro sperm quality and samples are functional in vivo.

Effect of l-carnitine on sperm quality during liquid storage of chicken semen

2011 ◽  
Vol 21 (5) ◽  
pp. 711-717 ◽  
Author(s):  
Saleh Tabatabaei ◽  
Ali Aghaei
Keyword(s):  
Sperm Quality ◽  
Liquid Storage

Protective effects of Opuntia ficus-indica extract on ram sperm quality, lipid peroxidation and DNA fragmentation during liquid storage

2016 ◽  
Vol 175 ◽  
pp. 1-9 ◽  
Author(s):  
Larbi Allai ◽  
Xavier Druart ◽  
Mehmet Öztürk ◽  
Anass BenMoula ◽  
Boubker Nasser ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Protective Effects ◽  
Opuntia Ficus Indica ◽  
Liquid Storage ◽  
Ram Sperm

scholarly journals The Antibacterial and Antioxidant Roles of Buckwheat Honey (BH) in Liquid Preservation of Boar Semen

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Qun Lan ◽  
Yingyu Xie ◽  
Jiahua Pan ◽  
Qiaohui Chen ◽  
Tianfang Xiao ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Antioxidant Properties ◽  
Membrane Integrity ◽  
Storage Period ◽  
Antibiotic Use ◽  
Quality Parameters ◽  
Progressive Motility ◽  
Liquid Storage ◽  
Boar Semen

In the present study, we hypothesized that buckwheat honey (BH) should be regarded as a potential alternative to antibacterial and antioxidant agent in liquid storage of boar semen. To this end, boar semen was firstly studied for in vitro dose tolerability to BH by measuring sperm progressive motility. The optimum progressive motility of boar spermatozoa was observed in extender with 0.5% and 0.6% BH addition. Afterward, sperm quality parameters, bacterial profile and composition, total antioxidant (T-AOC), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) levels of control, BH supplementation, antibiotics supplementation, and incorporated supplementation were compared during liquid storage period, to further investigate antibacterial and antioxidant properties of BH. The results showed that BH supplementation significantly improved sperm motility, acrosome integrity, plasma membrane integrity, inhibited opportunistic bacterial growth, and altered microbial compositions at the end of preservation. Additionally, T-AOC, SOD, and CAT levels were significantly higher in the BH supplementation group than those in the control and antibiotic supplementation group, whereas MDA level exhibited opposite change pattern. Importantly, BH addition to the extender was able to exert a synergistic effect in combination of antibiotic use. Our findings suggested that the appropriate concentrations (0.5% and 0.6%) of BH were added to the extender could act antibacterial and antioxidant roles in liquid preservation of boar semen.

scholarly journals Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Biology ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 300 ◽  
Author(s):  
Sara Sadeghi ◽  
Raquel Del Gallego ◽  
Balma García-Colomer ◽  
Ernesto A. Gómez ◽  
Jesús L. Yániz ◽  
...  
Keyword(s):  
Dilution Rate ◽  
Dna Fragmentation ◽  
Sperm Motility ◽  
Storage Temperature ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Effect Of Temperature ◽  
Sperm Dna Fragmentation ◽  
Liquid Storage ◽  
Duration Of Storage

The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 °C or 17 °C), durations (0, 24 and 48 h) and sperm concentrations (250 × 106 sperm/mL (1:2 dilution rate), 166.7 × 106 sperm/mL (1:3 dilution rate) or 50 × 106 sperm/mL (1:10 dilution rate)) on sperm motility and kinetics were studied. In the second experiment, the effect of temperature, sperm washing and concentration on sperm motility and DNA fragmentation was studied. Finally, the effect of sperm concentration and duration of storage at 5 °C on sperm motility, response to oxidative stress and MMP was examined. We found that refrigerated liquid storage of goat sperm impaired sperm quality, such as motility, MMP and response to oxidation, as storage time increased; however, sperm DNA fragmentation index was not significantly affected. Liquid storage at 5 °C preserved higher total motility than at 17 °C. Moreover, we observed that the reduction of sperm concentration below 500 × 106 sperm/mL did not seem to improve the quality of spermatozoa conserved in milk-based extender in the conditions tested.

Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17°C

2019 ◽  
Vol 90 (9) ◽  
pp. 1161-1169 ◽  
Author(s):  
Tian‐Yu Feng ◽  
Fa Ren ◽  
Qian Fang ◽  
Gui‐Chao Dai ◽  
Yu Li ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Bacterial Composition ◽  
Liquid Storage ◽  
Boar Sperm
Sign in / Sign up

Export Citation Format

Share Document