Enhancing the proportion of gluconic acid with a microbial community reconstruction method to improve the taste quality of Kombucha

Abstract Background and Aims Routine gastroesophagostomy has been shown to have adverse effects on the recovery of digestive functions and quality of life because patients typically experience reflux symptoms after proximal gastrectomy. This study was performed to assess the feasibility and quality of life benefits of a novel reconstruction method termed Roux-en-Y anastomosis plus antral obstruction (RYAO) following proximal partial gastrectomy. Methods A total of 73 patients who underwent proximal gastrectomy from June 2015 to June 2017 were divided into two groups according to digestive reconstruction methods [RYAO (37 patients) and conventional esophagogastric anastomosis with pyloroplasty (EGPP, 36 patients)]. Clinical data were compared between the two groups retrospectively. Results The mean operative time for digestive reconstruction was slightly longer in the RYAO group than in the EGPP group. However, the incidence of postoperative short-term complications did not differ between the RYAO and the EGPP groups. At the 6-month follow-up, the incidence rates of both reflux esophagitis and gastritis were lower in the RYAO group than in the EGPP group (P = 0.002). Additionally, body weight recovery was better in the RYAO group (P = 0.028). The scale tests indicated that compared with the patients in the EGPP group, the patients in the RYAO group had significantly reduced reflux, nausea and vomiting and reported improvements in their overall health status and quality of life (all P < 0.05). Conclusion RYAO reconstruction may be a feasible procedure to reduce postoperative reflux symptoms and the incidence of reflux esophagitis and gastritis, thus improving patient quality of life after proximal gastrectomy.

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Detecting Redox Potentials Using Porous Boron Nitride/ATP-DNA Aptamer/Methylene Blue Biosensor to Monitor Microbial Activities

Micromachines ◽

10.3390/mi13010083 ◽

2022 ◽

Vol 13 (1) ◽

pp. 83

Author(s):

Kai Guo ◽

Zirui Song ◽

Gaoxing Wang ◽

Chengchun Tang

Keyword(s):

Methylene Blue ◽

Microbial Community ◽

Boron Nitride ◽

Redox Potential ◽

Microbial Activity ◽

Potential Change ◽

Dna Aptamer ◽

Redox Potentials ◽

Microplate Reader

Microbial activity has gained attention because of its impact on the environment and the quality of people’s lives. Most of today’s methods, which include genome sequencing and electrochemistry, are costly and difficult to manage. Our group proposed a method using the redox potential change to detect microbial activity, which is rooted in the concept that metabolic activity can change the redox potential of a microbial community. The redox potential change was captured by a biosensor consisting of porous boron nitride, ATP-DNA aptamer, and methylene blue as the fluorophore. This assembly can switch on or off when there is a redox potential change, and this change leads to a fluorescence change that can be examined using a multipurpose microplate reader. The results show that this biosensor can detect microbial community changes when its composition is changed or toxic metals are ingested.

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Comparative Transcriptome Analysis Revealed the Influence of Sucrose on Flavor and Taste Quality of Coffea arabicaand C.canephora Varieties

Beverage Plant Research ◽

10.48130/bpr-2021-0011 ◽

2021 ◽

Vol 1 (0) ◽

pp. 1-9

Author(s):

Lifang Huang ◽

◽

Lisong Hu ◽

Yu Fan ◽

Xiaoyang Wang ◽

...

Keyword(s):

Transcriptome Analysis ◽

Coffea Arabica ◽

Taste Quality ◽

Comparative Transcriptome ◽

Comparative Transcriptome Analysis

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THE EFFECT OF DIETARY ENERGY-TO-PROTEIN RATIO VARIATION ON THE TASTE QUALITY OF BROILER CHICKEN MEAT

Journal of Food Quality ◽

10.1111/j.1745-4557.1988.tb00915.x ◽

1988 ◽

Vol 10 (5) ◽

pp. 299-310 ◽

Cited By ~ 1

Author(s):

D. BASKER ◽

S. ANGEL ◽

I BARTOV ◽

Z. WEINBERG

Keyword(s):

Broiler Chicken ◽

Chicken Meat ◽

Taste Quality ◽

Dietary Energy ◽

Protein Ratio ◽

Ratio Variation

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Insight into how fertilization strategies increase quality of grape (Kyoho) and shift microbial community

Environmental Science and Pollution Research ◽

10.1007/s11356-021-17759-x ◽

2022 ◽

Author(s):

Xunan Li ◽

Changbin Chu ◽

Sheng Ding ◽

Huawei Wei ◽

Shuhang Wu ◽

...

Keyword(s):

Microbial Community ◽

Insight Into

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Effects of Cellulase on the Chemical Compositions Characteristics and Microbial Community of Saline-alkali Land Alfalfa Silage

10.21203/rs.2.17221/v1 ◽

2019 ◽

Author(s):

Qiang Lu ◽

Gentu Ge ◽

Qiming Cheng ◽

Meiling Hou ◽

shan Yu Jia

Keyword(s):

Microbial Community ◽

Anaerobic Fermentation ◽

Alpha Diversity ◽

Chemical Compositions ◽

Diversity Analysis ◽

Microbial Network ◽

Before And After ◽

Alfalfa Silage ◽

The Difference

Abstract Background We aim to assess the nutritional quality of alfalfa in saline-alkali and the main fermenting microorganisms acting on alfalfa in saline-alkali soils.Results In this study, We tested the nutrient composition and microbial community of Zhongmu No. 3 (salt-tolerant) alfalfa, including the nutritional fermentation quality and microbial diversity analysis of alfalfa before and after ensiling (30 days and 60 days). Then we got closely genus that related to saline-alkali soils.The validity of the microbiological data was confirmed by alpha diversity analysis. From the Microbial network, we obtained the difference of the saline-type mantle type.Conclusions we found that the silage quality of Saline-alkali alfalfa can be ensured by anaerobic fermentation with addition. The LABs that play a major role in the saline alkaline alfalfa silage are Lactobacillus, lactococcus and enterococcus.

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Alleviating Continuous Monocropping Obstacle in Melon: Biological Elimination of Phenolic Acid

10.21203/rs.3.rs-127303/v1 ◽

2020 ◽

Author(s):

Huiqin Xie ◽

Yongli Ku ◽

Xiangna Yang ◽

Le Cao ◽

Xueli Mei ◽

...

Keyword(s):

Microbial Community ◽

Microbial Communities ◽

Phenolic Acids ◽

Fruit Quality ◽

High Performance ◽

Soil Microbial Communities ◽

Illumina Miseq ◽

Continuous Cropping ◽

Soil Microbial

Abstract Background: Melon (Cucumis melo L.) is one of the most important fruit crops grown in China. However, the yield and quality of melon have significantly declined under continuous cropping. Phenolic acids are believed to be associated with the continuous monocropping obstacle (CMO) and can influence plant microbe interactions. Coumaric acid (CA) is one of the major phenolic acids found in melon root exudates. The objectives of this study were to estimate the elimination of CA by the soil bacterium K3 as well as its effects on mitigating melon CMO. CA degradation was investigated by monitoring the CA retained in the growth medium using high performance liquid chromatography (HPLC). The effects of CA and K3 on rhizosphere soil microbial communities were investigated by the spread plate method and Illumina MiSeq sequencing. Furthermore, the effects of CA and K3 on melon seedling growth were measured under potted conditions. The changes in soil enzymes and fruit quality under K3 amendment were examined in a greenhouse experiment. Result:The results suggest that the addition of CA had the same result as the CMO, such as deterioration of the microbial community and slower growth of melon plants. HPLC and microbial analysis showed that K3 had a pronounced ability to decompose CA and could improve the soil microbial community environment. Soil inoculation with K3 agent could significantly improve the fruit quality of melon.Conclusion: Our results show that the effects of K3 in the soil are reflected by changes in populations and diversity of soil microbes and suggest that deterioration of microbial communities in soil might be associated with the growth constraint of melon in continuous monoculture systems.

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