Porous BMP-2 Immobilized PLGA/Glycol Chitosan Scaffold with Enhanced Hydrophilicity, Mineralization and Osteogenesis

Accelerating wound healing with minimized bacterial infection has become a topic of interest in the development of the new generation of tissue bio-adhesives. In this study, we fabricated a hydrogel system (MGC-g-CD-ic-TCS) consisting of triclosan (TCS)-complexed beta-cyclodextrin (β-CD)-conjugated methacrylated glycol chitosan (MGC) as an antibacterial tissue adhesive. Proton nuclear magnetic resonance (1H NMR) and differential scanning calorimetry (DSC) results showed the inclusion complex formation between MGC-g-CD and TCS. The increase of storage modulus (G’) of MGC-g-CD-ic-TCS after visible light irradiation for 200 s indicated its hydrogelation. The swollen hydrogel in aqueous solution resulted in two release behaviors of an initial burst and sustained release. Importantly, in vitro and in vivo results indicated that MGC-g-CD-ic-TCS inhibited bacterial infection and improved wound healing, suggesting its high potential application as an antibacterial tissue bio-adhesive.

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Effects of Velvet Antler Polypeptide-Collagen/Chitosan Materials on Proliferation of Osteoblast

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.380-384.4295 ◽

2013 ◽

Vol 380-384 ◽

pp. 4295-4298

Author(s):

Wen He Zhu ◽

Jun Jie Xu ◽

Wei Zhang ◽

Yan Li ◽

Xiao Jing Lu ◽

...

Keyword(s):

Drug Treatment ◽

Bone Defect ◽

Three Dimensional ◽

Chitosan Scaffold ◽

Release Drug ◽

Dimensional Network ◽

Velvet Antler ◽

Result Show ◽

Long Time ◽

Velvet Antler Polypeptide

A highly osteogenic hybrid bioabsorbable scaffold was developed for bone reconstruction. Though the use of a bioabsorbable collagen and chitosan scaffold for loading velvet antler polypeptide to repair bone defect and drug treatment. Velvet antler polypeptide and collagen were extracted for developing the compounded material. The SEM results show that the collagen and chitosan scaffold maintain the natural three dimensional network structures. The cell proliferation experiment result show that the can promote the osteoblast proliferation for a long time . These results indicated that this compound scaffold can sustainable to release drug and is a good material in bone defect and drug treatment.

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Theragnostic Glycol Chitosan-Conjugated Gold Nanoparticles for Photoacoustic Imaging of Regional Lymph Nodes and Delivering Tumor Antigen to Lymph Nodes

Nanomaterials ◽

10.3390/nano11071700 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1700

Author(s):

In-Cheol Sun ◽

SeongHoon Jo ◽

Diego Dumani ◽

Wan Su Yun ◽

Hong Yeol Yoon ◽

...

Keyword(s):

Gold Nanoparticles ◽

Lymph Node ◽

Lymph Nodes ◽

Cancer Immunotherapy ◽

Tumor Antigen ◽

Tumor Antigens ◽

Imaging System ◽

Superior Performance ◽

Glycol Chitosan ◽

Cervical Lymph Nodes

Lymph node mapping is important in cancer immunotherapy because the morphology of lymph nodes is one of the crucial evaluation criteria of immune responses. We developed new theragnostic glycol-chitosan-coated gold nanoparticles (GC-AuNPs), which highlighted lymph nodes in ultrasound-guided photoacoustic (US/PA) imaging. Moreover, the ovalbumin epitope was conjugated GC-AuNPs (OVA-GC-AuNPs) for delivering tumor antigen to lymph node resident macrophage. In vitro studies proved the vigorous endocytosis activity of J774A.1 macrophage and consequent strong photoacoustic signals from them. The macrophages also presented a tumor antigen when OVA-GC-AuNPs were used for cellular uptake. After the lingual injection of GC-AuNPs into healthy mice, cervical lymph nodes were visible in a US/PA imaging system with high contrast. Three-dimensional analysis of lymph nodes revealed that the accumulation of GC-AuNPs in the lymph node increased as the post-injection time passed. Histological analysis showed GC-AuNPs or OVA-GC-AuNPs located in subcapsular and medullar sinuses where macrophages are abundant. Our new theragnostic GC-AuNPs present a superior performance in US/PA imaging of lymph nodes without targeting moieties or complex surface modification. Simultaneously, GC-AuNPs were able to deliver tumor antigens to cause macrophages to present the OVA epitope at targeted lymph nodes, which would be valuable for cancer immunotherapy.

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