Energy transfer from fluorescein dye dispersed with CdS nanoparticles as evidenced by emission spectrum measurements

Two probes were synthesized which consist of fluorescent molecules conjugated to saturated hydrocarbon chains, 18 carbons long, to ensure their localization into cellular membranes. There is an overlap between the emission spectrum of one probe (donor) and the absorption spectrum of the other probe (acceptor). By the use of appropriate wavelengths it is possible to specifically excite the donor probe and record the fluorescence of the acceptor probe. Two cell populations, each labelled with one of the probes, were infected with a virus that causes cell fusion, mixed in equal proportions, and the fluorescence of the acceptor probe measured as a function of time after infection. An increase in fluorescence was observed beginning at the time of onset of cell fusion indicating a mixing of the fluorescent membrane molecules. An investigation of the distance dependence indicated that the increase in fluorescence was mainly due to resonance energy transfer and not to photon emission and reabsorption. Resonance energy transfer requires that the 2 probes be close together and that there be an overlap of the emission spectrum of the donor probe and the absorption spectrum of the acceptor probe. The possible application of this assay to other types of membrane fusion is noted.

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New insights into the binding behavior of lomefloxacin and human hemoglobin using biophysical techniques: binary and ternary approaches

New Journal of Chemistry ◽

10.1039/c9nj01048c ◽

2019 ◽

Vol 43 (21) ◽

pp. 8132-8145 ◽

Cited By ~ 41

Author(s):

Parisa Mokaberi ◽

Vida Reyhani ◽

Zeinab Amiri-Tehranizadeh ◽

Mohammad Reza Saberi ◽

Sima Beigoli ◽

...

Keyword(s):

Absorption Spectrum ◽

Energy Transfer ◽

Emission Spectrum ◽

High Probability ◽

Fluorescence Emission ◽

Fluorescence Emission Spectrum ◽

Human Hemoglobin ◽

Binding Behavior ◽

Biophysical Techniques

Demonstrates the overlap that had been induced between the fluorescence emission spectrum of Hb and the absorption spectrum of drugs, which has proved that there is a high probability to the occurrence of energy transfer from Hb and LMF in the absence and presence of NRF.

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Article

Canadian Journal of Physics ◽

10.1139/p98-047 ◽

1998 ◽

Vol 76 (10) ◽

pp. 759-770

Author(s):

M Musso ◽

F Fuso ◽

G De Filippo ◽

M Allegrini ◽

D Gruber ◽

...

Keyword(s):

Energy Transfer ◽

Emission Spectrum ◽

Pulsed Laser ◽

Level Population ◽

Sodium Atoms ◽

Energy Transfer Pathway

Transfer of excitation from cadmium to sodium atoms was investigated in anheat pipe oven illuminated by a pulsed laser tuned on the intercombinationtransition of cadmium. A rich emission spectrum with cadmium as well assodium lines was observed. Intramultiplet mixing distributes the initialexcited level population 5p3P1 to the nearby fine structurelevels 5p3P0,2 of the same multiplet. We have experimentalevidence that the energy transfer pathway is from Cd (5p3PJ) to Na(4p2PJ) which is collisionally rapidly converted toNa(3d2DJ), from which the strongest emission was observed.PACS No.: 34.90+q

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Photoluminescence and Energy Transfer Study of Eu3+ Codoped with CdS Nanoparticles in Silica Glass

Journal of Fluorescence ◽

10.1007/s10895-011-0984-2 ◽

2011 ◽

Vol 22 (1) ◽

pp. 505-510 ◽

Cited By ~ 3

Author(s):

L. Bokatial ◽

S. Rai

Keyword(s):

Energy Transfer ◽

Silica Glass ◽

Cds Nanoparticles ◽

Transfer Study

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Effect of directed energy transfer on the emission-spectrum kinetics of complex-molecule solid solutions

Journal of Applied Spectroscopy ◽

10.1007/bf00614041 ◽

1980 ◽

Vol 33 (6) ◽

pp. 1340-1343

Author(s):

N. A. Nemkovich ◽

I. M. Gulis ◽

V. I. Tomin

Keyword(s):

Energy Transfer ◽

Emission Spectrum ◽

Solid Solutions ◽

Complex Molecule ◽

Directed Energy ◽

Kinetics Of

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The Crab nebula energy origin and its high frequency radiation spectra

Journal of Plasma Physics ◽

10.1017/s0022377816000416 ◽

2016 ◽

Vol 82 (3) ◽

Cited By ~ 4

Author(s):

George Z. Machabeli ◽

A. Rogava ◽

N. Chkheidze ◽

Z. Osmanov ◽

D. Shapakidze

Keyword(s):

Energy Transfer ◽

Theoretical Model ◽

Emission Spectrum ◽

High Frequency ◽

Crab Nebula ◽

Synchrotron Emission ◽

Physical Processes ◽

Radiation Spectra ◽

The Crab Nebula ◽

Frequency Radiation

In the present work there is presented a model describing transfer of the Crab pulsar’s spin-down energy into the powerful synchrotron emission of the nebula. The process of the energy transfer consists of several consecutive stages. The physical processes underlying the theoretical model provide us with the synchrotron emission spectrum, which fits well with the observed one.

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