RETRACTED: Role of mammalian sirtuin 1 (SIRT1) in lipids metabolism and cell proliferation of goose primary hepatocytes

2014 ◽  
Vol 382 (1) ◽  
pp. 282-291 ◽  
Author(s):  
Chunchun Han ◽  
Huofu Wan ◽  
Shuang Ma ◽  
Dandan Liu ◽  
Fang He ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Sirtuin 1 ◽  
Primary Hepatocytes ◽  
Lipids Metabolism

scholarly journals Role of forkhead box protein O1 and insulin on cell proliferation mediated by sirtuin 1 in goose primary hepatocytes

2021 ◽  
Vol 30 (2) ◽  
pp. 100144
Author(s):  
Rongxue Wei ◽  
Chunchun Han ◽  
Fang He ◽  
Xiangping Xiong ◽  
Fengjiang Ye ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Sirtuin 1 ◽  
Primary Hepatocytes ◽  
Forkhead Box

scholarly journals Sirtuin 1 and Sirtuin 3 in Granulosa Cell Tumors

2021 ◽  
Vol 22 (4) ◽  
pp. 2047
Author(s):  
Nina Schmid ◽  
Kim-Gwendolyn Dietrich ◽  
Ignasi Forne ◽  
Alexander Burges ◽  
Magdalena Szymanska ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Drug Targets ◽  
Growth Regulation ◽  
Sirtuin 1 ◽  
Ki 67 ◽  
Granulosa Cell Tumors ◽  
Novel Drug

Sirtuins (SIRTs) are NAD+-dependent deacetylases that regulate proliferation and cell death. In the human ovary, granulosa cells express sirtuin 1 (SIRT1), which has also been detected in human tumors derived from granulosa cells, i.e., granulosa cell tumors (GCTs), and in KGN cells. KGN cells are an established cellular model for the majority of GCTs and were used to explore the role of SIRT1. The SIRT1 activator SRT2104 increased cell proliferation. By contrast, the inhibitor EX527 reduced cell numbers, without inducing apoptosis. These results were supported by the outcome of siRNA-mediated silencing studies. A tissue microarray containing 92 GCTs revealed nuclear and/or cytoplasmic SIRT1 staining in the majority of the samples, and also, SIRT2-7 were detected in most samples. The expression of SIRT1–7 was not correlated with the survival of the patients; however, SIRT3 and SIRT7 expression was significantly correlated with the proliferation marker Ki-67, implying roles in tumor cell proliferation. SIRT3 was identified by a proteomic analysis as the most abundant SIRT in KGN. The results of the siRNA-silencing experiments indicate involvement of SIRT3 in proliferation. Thus, several SIRTs are expressed by GCTs, and SIRT1 and SIRT3 are involved in the growth regulation of KGN. If transferable to GCTs, these SIRTs may represent novel drug targets.

scholarly journals Forkhead box protein O1 (FoxO1) regulates lipids metabolism and cell proliferation mediated by insulin and PI3K-Akt-mTOR pathway in goose primary hepatocytes

BIOCELL ◽  
2022 ◽  
Vol 46 (1) ◽  
pp. 171-183
Author(s):  
RONGXUE WEI ◽  
CHUNCHUN HAN ◽  
FENGJIANG YE ◽  
SHOUHAI WEI ◽  
FANG HE ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mtor Pathway ◽  
Primary Hepatocytes ◽  
Forkhead Box ◽  
Lipids Metabolism

scholarly journals ROLE OF SIRTUIN 1 IN REGULATION OF MELANOMA CELL PROLIFERATION

2020 ◽  
Vol 18 (6) ◽  
pp. 82-89
Author(s):  
I. Yu. Dubovtseva ◽  
M. V. Aksenenko ◽  
T. G. Ruksha
Keyword(s):  
Cell Proliferation ◽  
Melanoma Cell ◽  
Sirtuin 1 ◽  
Melanoma Cell Proliferation

scholarly journals Sirtuin 1 Protects the Mitochondria in Hepatocellular Carcinoma Cells via Suppressing Hypoxia-induced Factor-1 Alpha Expression

2022 ◽  
Author(s):  
Zun-Qiang Zhou ◽  
Jiao Guan ◽  
Shi-Geng Chen ◽  
Jian-Hua Sun ◽  
Zheng-Yun Zhang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Warburg Effect ◽  
Sirtuin 1 ◽  
Luciferase Reporter ◽  
Tumor Xenografts ◽  
Mitochondrial Functions ◽  
Hcc Cells ◽  
The Warburg Effect

Abstract Background: We hypothesized that Sirtuin 1 (SIRT1) might attenuate the Warburg effect in tumor cells by modulating hypoxia-induced factor-1 alpha (HIF-1α) expression. This study aimed to explore the role and the underlying mechanism of SIRT1 in protecting the mitochondrial functions in hepatocellular carcinoma (HCC) cells. Methods: Quantitative real-time PCR and western blot analysis were conducted to determine gene expression in HCC cells. Co-immunoprecipitation (co-IP), chromatin immunoprecipitation (ChIP), and luciferase reporter assays were performed to examine DNA-protein interactions. Colony formation and MTT assays were carried out to explore the role of SIRT1 in HCC cell proliferation in vitro. PLC5 and Huh7 tumor xenografts were generated in mice to investigate the role of SIRT1–HIF-1α signaling in HCC development in vivo. Results: In different HCC cell lines, overexpression of SIRT1 promoted oxidative phosphorylation-associated gene expressions, ATP production, cell proliferation, and apoptotic protein expression while attenuating VEGF expression. In mice, overexpression of SIRT1 resulted in significant reductions in the weights of PLC5 and Huh7 tumor xenografts. Knockdown of SIRT1 exhibited opposite effects. Mechanistically, overexpression of SIRT1 promoted HIF-1α deacetylation, VHL-mediated HIF-1α degradation, and AMPK expression. Furthermore, SIRT1 interfered with the HIF-1α–c-Myc interaction to stimulate the transcription of a mitochondrial biogenesis enhancer mitochondrial transcription factor A (TFAM). Overexpression of HIF-1α completely reversed the effects of SIRT1.Conclusions: SIRT1 protects the mitochondria of HCC cells via suppressing HIF-1α expression, suggesting that SIRT1 may exert antitumor activity in HCC by reducing the Warburg effect.

Essential role of Sharpin in TNFα dependent NFκB activation in primary hepatocytes

2012 ◽  
Vol 50 (01) ◽  
Author(s):  
N Lange ◽  
S Sieber ◽  
A Erhardt ◽  
G Sass ◽  
HJ Kreienkamp ◽  
...  
Keyword(s):  
Essential Role ◽  
Primary Hepatocytes

Use of riboflavinum and magnesium citrate in obstetrics and gynecology

GYNECOLOGY ◽  
2018 ◽  
Vol 20 (6) ◽  
pp. 60-66
Author(s):  
O A Gromova ◽  
I Yu Torshin ◽  
N K Tetruashvili
Keyword(s):  
Thrombus Formation ◽  
Sirtuin 1 ◽  
Obstetrics And Gynecology ◽  
Inflammatory Factor ◽  
Vitamin B2 ◽  
Magnesium Citrate ◽  
Fetal Malformations ◽  
Folate Cycle ◽  
Pro Inflammatory Cytokines

Low provision of cells with vitamin B2 and magnesium leads to a decrease in the activity of the sirtuin-1 deacetylase and an increase in the activity of the pro-inflammatory factor NF-kB, a decrease in the levels of glutathione, an increase in the levels of homocysteine, thrombus formation, the activity of mitochondria, the development of migraine, convulsions and miscarriage. The role of riboflavin in the regulation of the folate cycle in the genotype MTHFR 677TT for the prevention of folatresistant fetal malformations, the advantages of an aqueous solution of riboflavin and magnesium citrate is considered. The data on titanium dioxide, which increases the level of pro-inflammatory cytokines IL-1b, IL-4, IL-5, IL-6, G-CSF, CCL-2, CCL-3, CCL-4, are presented.

Sign in / Sign up

Export Citation Format

Share Document