Optimization of pH, time, temperature, variety and concentration of the added fatty acid and the initial count of added lactic acid Bacteria strains to improve microbial conjugated linoleic acid production in fermented ground beef

Meat Science ◽  
2021 ◽  
Vol 171 ◽  
pp. 108303
Author(s):  
Cem Okan Özer ◽  
Birol Kılıç
Author(s):  
Natalia Taboada ◽  
Carina Van Nieuwenhove ◽  
Roxana Medina ◽  
Soledad López Alzogaray

In this study the physicochemical, microbiological, and fatty acid compositions together with the specific esterase activities of semi-hard goat cheeses made from native strains as starter and adjunct cultures were evaluated and compared against those of manufactured using commercial culture cheeses. The physicochemical composition was similar among cheeses, while the lactic acid bacteria were the predominant microbiota in all samples. The highest specific esterase activities were detected in cheeses with native strains. The fatty acid profile was significantly affected by native strains during the ripening time (60 days) since the conjugated linoleic acid (CLA) level increased from 0.60 to 1.03 g 100 g-1 of fatty acids, whereas cheeses with commercial starter showed a CLA content of about 0.60 g of fatty acids. In cheeses with native strains, it was detected the highest desirable fatty acids, Δ9-desaturase and CLA desaturase indexes and the lowest atherogenicity index. The native strains inoculated as starter and adjunct cultures, grew conveniently in the cheese, developed their full potential as reflected by the profile of the metabolites released during ripening and in the global sensory perception of cheeses, and contributed thus to the development of a healthier food.


2018 ◽  
Vol 5 (5) ◽  
pp. 321-327
Author(s):  
Anas Ejaz Shaikh ◽  
Ankita Pawar ◽  
Hemant Parmar ◽  
Rajeshkumar N Vadgama ◽  
Arvind M Lali ◽  
...  

2002 ◽  
Vol 79 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Shigenobu Kishino ◽  
Jun Ogawa ◽  
Yoriko Omura ◽  
Kenji Matsumura ◽  
Sakayu Shimizu

2017 ◽  
Vol 6 (4) ◽  
pp. 44 ◽  
Author(s):  
Gabriela Christina Kuhl ◽  
Ana Paula Gusso ◽  
Brenda Lee Simas Porto ◽  
Carmen Maria Olivera Müller ◽  
Ricardo Ruiz Mazzon ◽  
...  

Thirty-five different lactic acid bacteria (LAB) strains were screened for their conjugated linoleic acid (CLA) isomers (C18:2 cis-9, trans-11 and C18:2 trans-10, cis-12) producing ability from linoleic acid (LA) in sheep’s milk. Preliminary experiments revealed that Lactobacillus delbrüeckii ssp. bulgaricus 2230 and Streptococcus thermophilus St 360 among the screened strains had the highest CLA-producing ability. This two strains were assayed in an 11-run fractional factorial design to investigate the effect of four variables included glucose, powdered sheep milk, LA and inoculum ratio on CLA production in a sheep’s milk yogurt. The optimum conditions for producing the highest levels of CLA (42.86%) were obtained by adding 10.00 mg/mL of glucose, 30.00 mg/mL of powdered sheep milk, 0.90 mg/mL of LA and a 1:2 (St:Lb) ratio of bacterial strains in the inoculum. This CLA-rich sheep’s milk yogurt could be an important supplementary food source for increasing the CLA in the human diet. 


2010 ◽  
Vol 63 (1) ◽  
pp. 62-69 ◽  
Author(s):  
MOHAMED H ABD EL-SALAM ◽  
KAWTHER EL-SHAFEI ◽  
OSAMA M SHARAF ◽  
BAHER A EFFAT ◽  
FAIZA M ASEM ◽  
...  

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