<p>Microbial metabolic functions and biogeochemical pathways of the complex rhizosphere-soil-microbe interactions change with aboveground vegetation and the ecosystem response to environmental changes. Soil trace gases and current genomic approaches have been valuable to characterize in-situ microbial activity. However, there is a lack of understanding of the complexity of the belowground processes, the time frame of microbial community responses to environmental changes and the degree to which microbial activity can be inferred current -omics approaches. In the nitrogen cycling at a field scale, microbial diversity or gene abundance sometimes does not explain N<sub>2</sub>O emissions or even gene expression, there some bacteria that cannot be cultivated, and in general &#8211;omics involve destructive soil sampling that is prone to changes of the in-situ soil conditions. Additionally, field soil sampling may not capture the heterogeneity of the soil or specific area of study.</p><p>Volatile Organic Compounds (VOCs) produced in the rhizosphere play an important role in microbial nutrient cycling. VOCs are produced by plants and microorganisms as a response to biotic or biotic stressors or the type of carbon sources available.</p><p>Here, we present how subsurface soil gas measurements in an enclosed ecosystem during the Water, Atmosphere, and Life Dynamics experiment (B2-WALD) at the Tropical Rainforest biome of Biosphere 2 (Arizona, USA) during an induced controlled drought. We present initial results of a unique non-destructive approach that simultaneously couples a) new hydrophobic-porous subsurface soil probes, b) high-resolution Tunable Infrared Laser Direct Absorption Spectrometers (TILDAS) to analyze in situ trace gas isotopomers, and c) a proton transfer reaction mass spectrometer (VOCUS, high resolution volatile organic compound gas analyzer) for VOC quantification. We measured soil gas isotopic composition of N<sub>2</sub>O and VOCs-- comparing rhizosphere and control areas before and during the drought. We will focus our discussion on VOCs and its potential as makers of microbial interactions and signaling as a response to an environmental stressor like drought.</p><p>In this project, we demonstrate the feasibility of online coupling of soil probes with high-resolution instrumentation to measure products from nitrogen cycling and nonmethane VOC production in soils as a response to soil-plant microbe interactions. In addition, this approach could be a potential tool to constraint inferences derived from different &#8211;omics approaches.</p>
Spatial metabolomics of in situ, host-microbe interactions