Extraction, isolation and characterization of bioactive compounds from chloroform extract of Carica papaya seed and it's in vivo antibacterial potentiality in Channa punctatus against Klebsiella PKBSG14

Aims: This study presents baseline data on the In-vitro and In-vivo evaluation of anti-trypanosomal activity of Carica papaya seed extracts and fractions in Albino Wister rats. Study Design: Mention the design of the study here. Place and Duration of Study Sample: Department of Biochemistry, Modibbo Adama University of Technology, Yola, between June 2009 and July 2010. Methodology: 56 Wister rats of both sexes were randomly divided into 8 groups (I – VIII) of 7 rats each were used for this study. Four concentrations (100, 200, 400 and 800 mg/kg) of different extracts of seed carica papaya were screened for trypanocidal activity against Trypanosoma brucei In vitro and In vivo. The effect of the extracts was evaluated for trypanocidal activity in rats infected and not infected with the parasite. Administration of the extract and the drugs was orally daily for 5 consecutive days from day 7 of post infection. Level of parasitemia and body weight was taken daily for 21 days. Results: The extracts inhibited parasite motility and totally eliminated the organisms at the concentrations used In vitro. The extract also showed promising in vivo trypanocidal activity. The observed In vitro and In vivo trypanocidal activities may be due to the presence of bioactive compounds present in the extracts as seen in this study. The extract also improved the observed decreases in haematological parameters of the treated rats, which may be due to their ability to decrease parasite load. The LD50 was estimated to be ≥2,000 mg/Kg (v/v) for acute oral toxicity test (because all the rats survived at the end of the 14-day observation period). This is an indication of very low toxicity, implying that the extract could be administered with some degree of safety. A significant decreased (p<0.05) were observed in weight of rats at 100 mg/kg, 200 mg/kg 400 mg/kg 800 mg/kg negative control and prophylactic at four to eight days of infections, while significantly increased (p<0.05) were observed in weight of rats for berenil control and normal control. Conclusion: The decrease in weight of rats could be as a result of loss of appetite due to severe fever and also the trypanosome.

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Proximate Composition and Phytochemical Constituents of Matured Carica papaya Seed Extracts

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2021/v9i130193 ◽

2021 ◽

pp. 28-33

Author(s):

M. A. Kanadi ◽

R. Yila ◽

M. P. Ibrahim ◽

A. I. Yaradua ◽

A. Nasir

Keyword(s):

Ethyl Acetate ◽

Proximate Composition ◽

Carica Papaya ◽

Chloroform Extract ◽

Phytochemical Analysis ◽

Crude Fibre ◽

Phytochemical Constituents ◽

Carica Papaya Seed ◽

Papaya Seed ◽

Seed Extracts

Aim: To investigate the proximate composition and phytochemical constituents of matured Carica papaya seed. Study Design: Proximate composition was determined on Carica papaya seed powder while extracts for phytochemical analysis were obtained, in turn using five different solvents of varying polarity, namely n-hexane, chloroform, ethyl acetate, methanol and water. Place and Duration of Study: Department of Biochemistry, Faculty of Basic Medical sciences, Bayero University Kano, Nigeria. The study was carried out between March- April, 2019. Results: The proximate composition of matured Carica papaya seed showed it contained crude fat (27.72%), carbohydrate (23.34%), crude fibre (21.25%), ash (10.25%), crude protein (9.65%) and moisture (7.34%). Qualitative phytochemical screening of Carica papaya seed extracts detected major phytochemicals except anthraquinones and quantitative analyses of these phytochemicals in all the five extracts showed that flavonoids was the most abundant phytochemical with 38.68%, 35.85%, 36.76%, 34.04% and 23.50% for methanol, aqueous, chloroform, n-hexane and ethyl acetate fractions respectively while tannins was the least abundant phytochemical with 0.03% for methanol extract, 0.04% for n-hexane extract, 0.09% for aqueous and ethyl acetate extracts, and 0.14% for the chloroform extract. Conclusion: Seeds of matured Carica papaya contain major nutrients that may be useful in nutrition. The presence of phytochemicals such as flavonoids, alkaloids and saponins in considerable quantities explained the medicinal activity of the plant material as encountered in its therapeutic uses.

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Effect of Aqueous extract on carica papaya seed and back on the Testes and sperm morphology of male wister rats

International Journal of Scientific and Research Publications (IJSRP) ◽

10.29322/ijsrp.9.09.2019.p9389 ◽

2019 ◽

Vol 9 (9) ◽

pp. p9389

Author(s):

Ovie F.O ◽

Ndukwe G.U ◽

Oliver N.L ◽

Obi K.C ◽

Aguwa U.S ◽

...

Keyword(s):

Aqueous Extract ◽

Carica Papaya ◽

Sperm Morphology ◽

Carica Papaya Seed ◽

Papaya Seed

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Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore.

The Journal of Cell Biology ◽

10.1083/jcb.121.3.513 ◽

1993 ◽

Vol 121 (3) ◽

pp. 513-519 ◽

Cited By ~ 63

Author(s):

W Jiang ◽

J Lechner ◽

J Carbon

Keyword(s):

Molecular Motors ◽

Cell Biology ◽

Budding Yeast ◽

Isolation And Characterization ◽

Sequence Homologies ◽

Binding Factor ◽

Microtubule Motor

We have cloned and determined the nucleotide sequence of the gene (CBF2) specifying the large (110 kD) subunit of the 240-kD multisubunit yeast centromere binding factor CBF3, which binds selectively in vitro to yeast centromere DNA and contains a minus end-directed microtubule motor activity. The deduced amino acid sequence of CBF2p shows no sequence homologies with known molecular motors, although a consensus nucleotide binding site is present. The CBF2 gene is essential for viability of yeast and is identical to NDC10, in which a conditional mutation leads to a defect in chromosome segregation (Goh, P.-Y., and J. V. Kilmartin, in this issue of The Journal of Cell Biology). The combined in vitro and in vivo evidence indicate that CBF2p is a key component of the budding yeast kinetochore.

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Isolation and characterization of adrenocortical progenitors involved in the adaptation to stress

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1814072115 ◽

2018 ◽

Vol 115 (51) ◽

pp. 12997-13002 ◽

Cited By ~ 15

Author(s):

Charlotte Steenblock ◽

Maria F. Rubin de Celis ◽

Luis F. Delgadillo Silva ◽

Verena Pawolski ◽

Ana Brennand ◽

...

Keyword(s):

Lineage Tracing ◽

Differentiated Cells ◽

Isolation And Characterization ◽

Proliferation And Differentiation ◽

Response To Stress ◽

Steroidogenic Cells ◽

High Degree

The adrenal gland is a master regulator of the human body during response to stress. This organ shows constant replacement of senescent cells by newly differentiated cells. A high degree of plasticity is critical to sustain homeostasis under different physiological demands. This is achieved in part through proliferation and differentiation of adult adrenal progenitors. Here, we report the isolation and characterization of a Nestin+ population of adrenocortical progenitors located under the adrenal capsule and scattered throughout the cortex. These cells are interconnected with progenitors in the medulla. In vivo lineage tracing revealed that, under basal conditions, this population is noncommitted and slowly migrates centripetally. Under stress, this migration is greatly enhanced, and the cells differentiate into steroidogenic cells. Nestin+ cells cultured in vitro also show multipotency, as they differentiate into mineralocorticoid and glucocorticoid-producing cells, which can be further influenced by the exposure to Angiotensin II, adrenocorticotropic hormone, and the agonist of luteinizing hormone-releasing hormone, triptorelin. Taken together, Nestin+ cells in the adult adrenal cortex exhibit the features of adrenocortical progenitor cells. Our study provides evidence for a role of Nestin+ cells in organ homeostasis and emphasizes their role under stress. This cell population might be a potential source of cell replacement for the treatment of adrenal insufficiency.

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Pharmaceutical properties of 'sucupira' (Pterodon spp.)

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502010000400002 ◽

2010 ◽

Vol 46 (4) ◽

pp. 607-616 ◽

Cited By ~ 15

Author(s):

Daiane Hansen ◽

Mitsue Haraguchi ◽

Antonio Alonso

Keyword(s):

Biological Effects ◽

Chemical Compounds ◽

Experimental Models ◽

Popular Medicine ◽

Isolation And Characterization ◽

Pharmaceutical Properties ◽

The Relationship

The plant of the genus Pterodon (Fabaceae, Leguminosae), commonly known as 'sucupira' or 'faveira', are disseminated throughout the central region of Brazil and has frequently been used in popular medicine for its anti-rheumatic, analgesic, and anti-inflammatory properties. In recent years, interest in these plants has increased considerably. The biological effects of different phytoextracts and pure metabolites have been investigated in several experimental models in vivo and in vitro. The literature describes flavonoids, triterpene and steroids, while one paper presented studies with proteins isolated from the genus. This review provides an overview of phytochemical and pharmacological research in Pterodon, showing the main chemical compounds studied to date, and focusing on the relationship between these molecules and their biological activity. Furthermore, this study paves the way for more in-depth investigation, isolation and characterization of the molecules of this plant genus.

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