Plant mitochondria under a variety of temperature stress conditions

Mitochondrion ◽  
2014 ◽  
Vol 19 ◽  
pp. 289-294 ◽  
Author(s):  
Michał Rurek
Keyword(s):  
Temperature Stress ◽  
Plant Mitochondria ◽  
Stress Conditions

scholarly journals Rotating tomography Paris–Edinburgh cell: a novel portable press for micro-tomographic 4-D imaging at extreme pressure/temperature/stress conditions

2016 ◽  
Vol 36 (4) ◽  
pp. 512-532 ◽  
Author(s):  
J. Philippe ◽  
Y. Le Godec ◽  
M. Mezouar ◽  
M. Berg ◽  
G. Bromiley ◽  
...  
Keyword(s):  
Temperature Stress ◽  
Stress Conditions ◽  
Extreme Pressure

scholarly journals Transcriptome-based identification and validation of optimal reference genes for quantitative real-time PCR normalization in Psathyrostachys huashanica

2020 ◽  
Author(s):  
Chuan Shen ◽  
Jingyuan Li ◽  
Caiyan Wei ◽  
Xudong Zhang ◽  
Yunfeng Wu
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Real Time ◽  
Temperature Stress ◽  
Reference Genes ◽  
18S Rrna ◽  
Stress Conditions ◽  
Psathyrostachys Huashanica ◽  
Biotic And Abiotic Stress ◽  
Ubiquitin Conjugating Enzyme

Abstract Background: P. huashanica ( Psathyrostachys huashanica ), known as an important resistance resource reservoir, is a rare and endangered plant growing suitably in Huashan mount region and would be urgently exploited in wheat genetic improvements sooner. During the utilization process, different IRGs (internal reference genes) need to be appropriately selected as standards based on biotic and abiotic stress conditions. It is crucial that Real-time RT-qPCR with combination of bioinformatics were adopted to explore the reliable IRGs from transcriptome of P . huashanica.Results: The present work reported new 3 species of IRGs, UBC2 , UBC17, 18S rRNA , which were screened from transcriptome of P. huashanica under biotic and abiotic stress conditions, using RT-qPCR and four algorithms, including geNorm, NormFinder, BestKeeper, and RefFinder, to analyse expression of sixteen candidate reference genes. These genes appear as following 18S rRNA (18S ribosomal RNA), EF1-α (eukaryotic elongation factor 1 alpha), UBC2 (ubiquitin-conjugating enzyme E2-2), UBC17 (ubiquitin-conjugating enzyme E2-17), α-TUB2A (alpha tubulin-2A), β-TUB3 (beta tubulin 3), ADF4 (Actin-depolymerising factor 4), ACTIN (actin), GAPDH (Glyceraldehyde-3-phosphate dehydrogenase), 60SARP (60S acidic ribosomal protein), UBQ (polyubiquitin), SamDC (S-Adenosylmethionine decarboxylase), EIF4A (eukaryotic initiation factor 4A), ARF (ADP-ribosylation factor), HIS1 (histone H1), and HIS2B (histone H2B). Analysis of gene expression demonstrated that the expression of UBC2 gene was most stable under ABA hormone stress, low temperature stress and high temperature stress, similarly, UBC17 gene under IAA hormone stress, salinity stress and drought stress, both UBC17 genes and 18S rRNA genes under abiotic and biotic stress, respectively. The most stable gene was UBC2 gene in the root, UBC17 gene in stem and leaf. In this study, α-TUB2A , UBC and ACTIN genes were verified as the suitable reference genes across all tested samples. To further validate the suitability of the selected reference genes, we evaluated the relative expression of PsaCPK3 (Calcium-dependent protein kinase) and PsaHSP70-1 (heat shock protein 70-1), which are stress-related genes that may be involved in response to adversity.Conclusions: This study has identified a set of the most stable IRGs suiting for RT-qPCR detection of a few target gene expressions from P . huashanica in different experimental conditions. In addition, this study should provide the accuracy information for gene expression analysis in P . huashanica .

scholarly journals Vermicompost priming stimulated germination and seedling emergence of onion seeds against abiotic stresses

2020 ◽  
Vol 48 (2) ◽  
pp. 153-157
Author(s):  
S.H. Muhie ◽  
E. Yildirim ◽  
N. Memis ◽  
I. Demir
Keyword(s):  
Superoxide Dismutase ◽  
Temperature Stress ◽  
Seedling Growth ◽  
Ascorbate Peroxidase ◽  
Abiotic Stresses ◽  
Seedling Emergence ◽  
Stress Conditions ◽  
Onion Seed ◽  
Different Levels

The present study elucidates the effect of matrix priming with vermicompost (VCP) on germination and seedling emergence of two onion seed lots under drought, salt and temperature stress conditions. Seeds treated with water was considered as hydropriming (HP) and untreated (NP) seeds were taken as control. Treated and untreated seeds were exposed to different levels of drought (10 and 15% PEG), salt (50 and 100 mM NaCl) and temperature (30 and 35°C) stresses. Priming with vermicompost improved the performance of onion seed as indicated by higher germination and seedling growth under stress conditions. Catalase, superoxide dismutase and ascorbate peroxidase activities were found to be significantly (P < 0.05) higher in the vermicompost treated seeds than hydroprimed and untreated seeds.

scholarly journals Morphological, Physiological and Biochemical Response to Low Temperature Stress in Tomato (Solanum lycopersicum L.): A Review

2021 ◽  
Vol 12 (6) ◽  
pp. 706-712
Author(s):  
D. K. Yadav ◽  
◽  
Yogendra K. Meena ◽  
L. N. Bairwa ◽  
Uadal Singh ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Low Temperature ◽  
Temperature Stress ◽  
Tomato Plant ◽  
Reactive Oxygen ◽  
Pollen Sterility ◽  
Low Temperature Stress ◽  
Stress Conditions ◽  
Oxygen Species ◽  
Physiological And Biochemical

Growth and productivity are traumatized by the low temperature that triggers a series of physiological, morphological, molecular and biochemical changes in plants that eventually disturb plant life. Most of the cultivable lands of the world are adversely affected by temperature stress conditions which have an adverse impact on global tomato productivity. Plants undergo several water related metabolic activities for their survival during cold stress conditions. Understanding the morphological, physiological and biochemical reactions to low temperature is essential for a comprehensive view of the perception of tomato plant tolerant mechanism. This review reports some aspects of low temperature inflated changes in physiological and biochemical in the tomato plant. Low temperature stress influences the reproductive phases of plants with delayed flowering which enhance pollen sterility resultant drastically affects the harvest yield. It also decreases the capacity and efficiency of photosynthesis through changes in gas exchange, pigment content, chloroplast development and decline in chlorophyll fluorescence photosynthetic attributes. Amassing of osmoprotectant is another adaptive mechanism in plants exposed to low temperatures stress, as essential metabolites directly participate in the osmotic adjustment. Furthermore, low temperature stress enhanced the production of reactive oxygen species (ROS) which may oxidize lipids, proteins and nucleic acids which bring in distortion at the level of the cell. At the point when extreme reactive oxygen species produced, plants synthesize antioxidant enzymes and osmoprotectants that quench the abundance of reactive oxygen species. These reviews focus on the capacity and techniques of the tomato plant to react low temperature stress.

Identification of regulatory regions and regulatory protein complexes of the Spirulina desD gene under temperature stress conditions: Role of thioredoxin as an inactivator of a transcriptional repressor GntR under low-temperature stress

2012 ◽  
Vol 90 (5) ◽  
pp. 621-635 ◽  
Author(s):  
Pavinee Kurdrid ◽  
Phuttawadee Phuengcharoen ◽  
Rayakorn Yutthanasirikul ◽  
Sittiruk Roytrakul ◽  
Atchara Paemanee ◽  
...  
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Protein Complex ◽  
Protein Complexes ◽  
Regulatory Protein ◽  
Regulatory Elements ◽  
Stress Conditions ◽  
Upstream Region ◽  
Regulatory Regions ◽  
Temperature Responsive

In the present study, electrophoretic mobility shift assays were used to identify temperature responsive elements in the 5′ upstream region (5′ UTR) of the Spirulina desD gene. Overlapping, synthetic oligonucleotides of both sense and anti-sense strands that spanned the entire 5′ UTR of the gene were analyzed. The responsive DNA-binding protein complexes were identified using liquid chromatography–tandem mass spectrometry. The results indicated that the cold-responsive elements were located at –453 to –247, –197 to –151, –105 to –76, and –50 to –1, whereas the low-temperature specific regulatory regions were located at –372 to –352. Moreover, the heat-responsive elements were located at –347 to –243, –197 to –151, and –124 to –1, whereas the high-temperature specific elements were located between –130 to –101 and –30 to –1. In terms of regulatory protein complexes under the two stress conditions, Trx was only detected in the low-temperature responsive protein complex, and divalent cations were essential for the binding of the protein complex to the regulatory elements. Furthermore, Trx was shown to play a critical role as a reducing agent that inactivates the Spirulina desD repressor, GntR. Consequently, the desD gene expression is induced under the low-temperature condition.

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