Allogenic bone grafts in post-traumatic juxta-articular defects: Need for allogenic bone banking

Bone grafts have been used to fill bone defects caused by disease or trauma. The amount of autografts is limited and allogenic bone grafts may transmit diseases and cause immune responses. Numerous materials have been proposed and used as scaffolds for bone tissue reconstruction. In this study, we tested nanophase PLGA/HA composite with mesenchymal stem cells in vitro to examine its biological response and cellular activity. The nanophase composite was compared to conventional polystyrene on cytocompatibility by cell attachment, proliferation, alkaline phosphotase activity test and scanning electron microscopy (SEM) analysis. The results demonstrated that human mesenchymal cells showed more cell attachment and higher cell proliferation rate when growing on nanophase PLGA/HA composite than those growing on polystyrene alone. And the composite also promoted MSC cells differentiate to osteoblast cells as compared with control. It was suggested that the combination of bone marrow mesenchymal cells with artificial materials or differentiation factors may enhance bone formation and regeneration, nanophase PLGA/HA composite might therefore be a promising scaffold material for bone tissue substitute in clinical application.

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Quantification of growth factors in allogenic bone grafts extracted with three different methods

Cell and Tissue Banking ◽

10.1007/s10561-006-9021-0 ◽

2006 ◽

Vol 8 (2) ◽

pp. 107-114 ◽

Cited By ~ 47

Author(s):

B. Wildemann ◽

A. Kadow-Romacker ◽

A. Pruss ◽

N. P. Haas ◽

G. Schmidmaier

Keyword(s):

Growth Factors ◽

Bone Grafts ◽

Allogenic Bone

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Osteochondral reconstruction for post-traumatic coronoid deficiency

Journal of Orthopaedic Surgery ◽

10.1177/2309499020968606 ◽

2020 ◽

Vol 28 (3) ◽

pp. 230949902096860

Author(s):

Erica Kholinne ◽

Jae-Man Kwak ◽

Hyojune Kim ◽

Yucheng Sun ◽

Kyoung-Hwan Koh ◽

...

Keyword(s):

Clinical Outcome ◽

Bone Graft ◽

Graft Survival ◽

Bone Grafts ◽

Secondary Osteoarthritis ◽

Osteochondral Grafts ◽

Radiologic Evaluation ◽

Post Traumatic ◽

The Mean

Purpose: This study aimed to evaluate the clinical outcome and graft survival following coronoid reconstruction with osteochondral bone grafts for post-traumatic coronoid deficiency treatment. We hypothesized that coronoid reconstruction using an osteochondral bone graft will provide favorable results in treating post-traumatic coronoid deficiency. Methods: A retrospective review was performed on eight patients (mean age = 45.8 years) who underwent osteochondral bone graft reconstruction indicated for post-traumatic coronoid deficiency. The osteochondral bone grafts were obtained from the radial head remnant (four patients), olecranon tip (two patients), and iliac crest (two patients). All the injuries were terrible triad. The mean duration from injury to surgery was 79.3 weeks. The visual analog scale (VAS) for pain, motion arc, and Mayo elbow performance score (MEPS) were used to evaluate the clinical outcome. Radiologic evaluation of graft healing and integrity was performed using computed tomography at 19 months and plain elbow radiography at 24.1 months after reconstruction. The immediate graft height was measured. Results: VAS and MEPS values improved from 4.1 ± 1.2 to 1.1 ± 0.3 and 34.2 ± 16.9 to 85.0 ± 7.1, respectively ( p = 0.018, p = 0.018) after reconstruction. The motion arc significantly improved from 84.2° ± 16.1° to 102.1° ± 18.2° at the final follow-up of 39.1 ± 18.8 months ( p = 0.048). All the osteochondral grafts survived, with nonunion in two patients (25%). The mean immediate graft height was 15.4 ± 2.6 mm. Among the eight patients, three (37.5%) developed secondary osteoarthritis of the ulnohumeral joint. Conclusions: Coronoid reconstruction with osteochondral bone graft may serve as an option to salvage post-traumatic coronoid deficiency. Sufficient graft height was required for graft survival. Secondary osteoarthritis of the ulnohumeral joint should not be underestimated during follow-up.

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Prefabrication of Vascularized Allogenic Bone Graft in a Rat by Implanting a Flow-Through Vascular Pedicle and Basic Fibroblast Growth Factor Containing Hydroxyapatite/Collagen Composite

Journal of Reconstructive Microsurgery ◽

10.1055/s-0037-1599077 ◽

2017 ◽

Vol 33 (05) ◽

pp. 367-376 ◽

Cited By ~ 4

Author(s):

Konosuke Yamaguchi ◽

Osamu Nakamura ◽

Sachiko Tobiume ◽

Tetsuji Yamamoto ◽

Yoshio Kaji

Keyword(s):

Growth Factor ◽

Bone Resorption ◽

Bone Formation ◽

Bone Graft ◽

Bone Grafts ◽

Control Group ◽

Vascular Bundles ◽

Fibroblast Growth ◽

Allogenic Bone ◽

Flow Through

Background Basic fibroblast growth factor (bFGF) is known to stimulate bone formation and angiogenesis. Hydroxyapatite/collagen composite (HAp/Col) is also known to have very strong bone conductive activity. In this study, prefabrication of vascularized allogenic bone (allo-bone) graft was attempted in recipients by implanting vascular bundles from recipients into the transplanted allo-bone graft. Furthermore, the effect of bFGF-containing HAp/Col on the prefabricated vascularized allo-bone graft was investigated. Methods In this study, 32 Sprague-Dawley rats were used as donors, and bone grafts were collected from their femora. Thirty-two Wistar rats (recipients) were divided into four groups, and the allo-bone grafts were transplanted into the thigh region. In the experimental groups, one or both of the flow-through saphenous vascular bundles and 100-μg bFGF-containing HAp/Col were implanted into the medullary cavity of the allo-bone grafts. In the control group, neither was implanted. These rats were sacrificed at 4 weeks after transplantation, and bone formation, angiogenesis, and bone resorption in the transplanted allo-bone grafts were evaluated histologically and genetically. Results Bone formation and angiogenesis in the transplanted allo-bone graft were effectively stimulated by implanting vascular bundles or bFGF-containing HAp/Col on both histological and genetic evaluations compared with the control group. The most significant stimulation was observed in the group in which both were implanted. Bone resorption was not stimulated in any group. Conclusion By implanting a flow-through vascular bundle and bFGF-containing HAp/Col, an ideal vascularized allo-bone graft that had high bone formative and angiogenetic activities and did not stimulate bone resorptive activity was prefabricated.

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Bone Grafts in Jaw Cysts- Hydroxyapatite & Allogenic Bone – A Comparative Study

Bangabandhu Sheikh Mujib Medical University Journal ◽

10.3329/bsmmuj.v2i1.3707 ◽

2009 ◽

Vol 2 (1) ◽

Cited By ~ 1

Author(s):

Showkat Mamun ◽

Mahmuda Akhter ◽

Motiur Rahman Molla

Keyword(s):

Comparative Study ◽

Bone Grafts ◽

Jaw Cysts ◽

Allogenic Bone

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Experimental study on allogenic bone grafts

International Orthopaedics ◽

10.1007/bf00179718 ◽

1991 ◽

Vol 15 (2) ◽

Cited By ~ 8

Author(s):

M. Itoman ◽

S. Nakamura

Keyword(s):

Experimental Study ◽

Bone Grafts ◽

Allogenic Bone

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A new method of prefabricated vascularized allogenic bone grafts for maxillary and mandibular reconstruction: The prefabricated alloqenic osteo-fascio-cutaneous flap

International Journal of Oral and Maxillofacial Surgery ◽

10.1016/s0901-5027(99)81017-0 ◽

1999 ◽

Vol 28 ◽

pp. 131

Author(s):

P. Choung

Keyword(s):

Bone Grafts ◽

Mandibular Reconstruction ◽

New Method ◽

Allogenic Bone ◽

Cutaneous Flap

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A partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models

Scientific Reports ◽

10.1038/s41598-021-84039-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Pierre Tournier ◽

Jérôme Guicheux ◽

Arnaud Paré ◽

Aymeric Maltezeanu ◽

Thibaut Blondy ◽

...

Keyword(s):

Bone Graft ◽

Bone Regeneration ◽

Bone Healing ◽

Bone Grafts ◽

Ease Of Use ◽

Osteogenic Potential ◽

Allogeneic Bone ◽

Promising Alternative ◽

Allogenic Bone

AbstractIn skeletal surgical procedures, bone regeneration in irregular and hard-to-reach areas may present clinical challenges. In order to overcome the limitations of traditional autologous bone grafts and bone substitutes, an extrudable and easy-to-handle innovative partially demineralized allogenic bone graft in the form of a paste has been developed. In this study, the regenerative potential of this paste was assessed and compared to its clinically used precursor form allogenic bone particles. Compared to the particular bone graft, the bone paste allowed better attachment of human mesenchymal stromal cells and their commitment towards the osteoblastic lineage, and it induced a pro-regenerative phenotype of human monocytes/macrophages. The bone paste also supported bone healing in vivo in a guide bone regeneration model and, more interestingly, exhibited a substantial bone-forming ability when implanted in a critical-size defect model in rat calvaria. Thus, these findings indicate that this novel partially demineralized allogeneic bone paste that combines substantial bone healing properties and rapid and ease-of-use may be a promising alternative to allogeneic bone grafts for bone regeneration in several clinical contexts of oral and maxillofacial bone grafting.

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