scholarly journals Diagnosis and management of a fatal case of sepsis caused by Candida parapsilosis sensu stricto in a neonate with omphalocele

2018 ◽  
Vol 20 ◽  
pp. 10-14
Author(s):  
Simone Santana ◽  
Tania Salci ◽  
Patricia Andriato ◽  
Patricia Bonfim-Mendonça ◽  
Silvana Caparroz-Assef ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Fatal Case ◽  
Sensu Stricto ◽  
Diagnosis And Management

scholarly journals A case series of medically managed Candida parapsilosis complex prosthetic valve endocarditis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Penghao Guo ◽  
Yuting He ◽  
Rui Fan ◽  
Zhongwen Wu ◽  
Yili Chen ◽  
...  
Keyword(s):  
Prosthetic Valve ◽  
Candida Parapsilosis ◽  
Case Series ◽  
Prosthetic Valve Endocarditis ◽  
Sensu Stricto ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Tof Ms

Abstract Background In recent years, Candida parapsilosis is recognized as a species complex and is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis. Candida parapsilosis complex prosthetic valve endocarditis (PVE) is rare and the survival rate is still low despite of optimal therapeutic strategies. In our report, it is novel to report cases as Candida parapsilosis complex PVE at species and identify Candida parapsilosis using MALDI-TOF MS. Case presentation A series of 4 cases of Candida parapsilosis complex PVE from our institution was reported. Three were infected by Candida parapsilosis sensu stricto and one was infected by Candida metapsilosis. The condition of two cases got better and the other died. Conclusions More attention should be paid to Candida parapsilosis complex PVE and early diagnosis and prompt antibiotic therapy may play a role in the treatment for Candida parapsilosis complex PVE. It is recommended to identify Candida parapsilosis complex at species level and MALDI-TOF MS as an easy, fast and efficient identification method is worth promoting in clinical microbiology

scholarly journals Rapid Detection of Echinocandins Resistance by MALDI-TOF MS in Candida parapsilosis Complex

2020 ◽  
Vol 8 (1) ◽  
pp. 109
Author(s):  
Ana Emília M. Roberto ◽  
Danilo E. Xavier ◽  
Esteban E. Vidal ◽  
Cláudia Fernanda de L. Vidal ◽  
Rejane P. Neves ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Incubation Time ◽  
Candida Parapsilosis ◽  
Clinical Laboratory ◽  
Antifungal Susceptibility ◽  
Reference Method ◽  
Sensu Stricto ◽  
Maldi Tof ◽  
Separation Parameter ◽  
Candida Parapsilosis Complex

Mass spectrometry by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) was used to identify and differentiate the pattern of susceptibility of clinical isolates of Candida parapsilosis complex. 17 C. parapsilosis sensu stricto, 2 C. orthopsilosis, and 1 C. metapsilosis strains were obtained from blood cultures, and three different inocula (103, 105, and 107 CFU/mL) were evaluated against three echinocandins at concentrations ranging from 0.03 to 16 µg/mL after incubation of 1 h, 2 h, and 3 h. Drug-free control was used. The spectra obtained at these concentrations were applied to generate composite correlation index (CCI) matrices for each yeast individually. After cross correlations and autocorrelations of each spectra with null (zero) and maximal (16) concentrations, the CCI was used as separation parameter among spectra. Incubation time and inoculum were critical factors to reach higher precision and reliability of this trial. With an incubation time of 3 h and inoculum of 107 CFU/mL, it was possible to determine the breakpoint of the clinical yeasts by MALDI-TOF that presented high agreement with the clinical laboratory standard institute (CLSI) reference method. Herein, we show that mass spectrometry using the MALDI-TOF technique is powerful when it exploits antifungal susceptibility testing assays.

scholarly journals New species within Candida parapsilosis and Candida glabrata

2019 ◽  
pp. 51-57
Author(s):  
Dariusz Domański ◽  
Magdalena Anna Sikora ◽  
Robert Tomasz Kuthan ◽  
Ewa Augustynowicz-Kopeć ◽  
Ewa Swoboda-Kopeć
Keyword(s):  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Secondary Alcohol ◽  
Sensu Stricto ◽  
Diagnostic Methods ◽  
D2 Domain ◽  
Dehydrogenase Gene ◽  
Pcr Rflp ◽  
Secondary Alcohol Dehydrogenase ◽  
Candida Nivariensis

Introduction: Candida parapsilosis and Candida glabrata are another yeasts that form complexes of crypospecies. Although these species have been described more than a decade ago, knowledge about them is still limited. The reason for this is the large phenotypic similarity that unables them from being differentiated by classical diagnostic methods. The aim of the study was to identify species of clinical strains within C. glabrata and C. parapsilosis complexes. Material and methods: Standard PCR-RFLP of the secondary alcohol dehydrogenase gene (SADH) with BanI restriction enzyme served to determine species affiliation within the C. parapsilosis complex. The internal transcribed spacer was used to confirm the identification of C. glabrata sensu stricto. The D1/D2 domain of the 26S rDNA gene was sequenced in order to identify C. nivariensis and C. bracarensis strains. Results: As a result of the molecular analysis, 24 Candida nivariensis isolates and 4 C. metapsilosis strains and 9 C. orthopsilosis strains were detected. Conclusions: Prevalence of new cryptic species was relatively low.

The serine peptidase inhibitor TPCK induces several morphophysiological changes in the opportunistic fungal pathogen Candida parapsilosis sensu stricto

2019 ◽  
Vol 57 (8) ◽  
pp. 1024-1037 ◽  
Author(s):  
Rafael M Gandra ◽  
Laura N Silva ◽  
Xênia M Souto ◽  
Leandro S Sangenito ◽  
Lucas P S Cruz ◽  
...  
Keyword(s):  
Cell Biology ◽  
Candida Parapsilosis ◽  
Galleria Mellonella ◽  
Neutral Lipids ◽  
Sensu Stricto ◽  
New Drugs ◽  
Candida Spp ◽  
Cytoplasmic Inclusions ◽  
Chloromethyl Ketone ◽  
Serine Peptidase

AbstractCandida parapsilosis sensu stricto (C. parapsilosis) has emerged as the second/third commonest Candida species isolated from hospitals worldwide. Candida spp. possess numerous virulence attributes, including peptidases that play multiple roles in both physiological and pathological events. So, fungal peptidases are valid targets for new drugs development. With this premise in mind, we have evaluated the effect of serine peptidase inhibitors (SPIs) on both cell biology and virulence aspects of C. parapsilosis. First, five different SPIs, phenylmethylsulfonyl fluoride, benzamidine, 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride, N-α-tosyl-L-lysine chloromethyl ketone hydrochloride, and N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) were tested, and TPCK showed the best efficacy to arrest fungal growth. Subsequently, the ability of TPCK to modulate physiopathological processes was investigated. Overall, TPCK was able to (i) inhibit the cell-associated serine peptidase activities, (ii) promote morphometric and ultrastructural alterations, (iii) induce an increase in the intracellular oxidation level, which culminates in a vigorous lipid peroxidation and accumulation of neutral lipids in cytoplasmic inclusions, (iv) modulate the expression/exposition of surface structures, such as mannose/glucose-rich glycoconjugates, N-acetylglucosamine-containing molecules, chitin, polypeptides and surface aspartic peptidases, (v) reduce the adhesion to either polystyrene or glass surfaces as well as to partially disarticulate the mature biofilm, (vi) block the fungal interaction with macrophages, and (vii) protect Galleria mellonella from fungal infection, enhancing larvae survivability. Altogether, these results demonstrated that TPCK induced several changes over fungal biology besides the interference with aspects associated to C. parapsilosis virulence and pathogenesis, which indicates that SPIs could be novel promising therapeutic agents in dealing with candidiasis.

scholarly journals High prevalence of Itraconazole Resistance among Candida parapsilosis isolated from Iran

2019 ◽  
Author(s):  
Fozieh Hassanmoghadam ◽  
Tahereh Shokohi ◽  
Mohammad Taghi Hedayati ◽  
Narges Aslani ◽  
Iman Haghani ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Inhibitory Concentration ◽  
Antifungal Susceptibility ◽  
Reference Method ◽  
Sensu Stricto ◽  
Antifungal Drugs ◽  
High Prevalence ◽  
Invasive Infections ◽  
Prophylaxis Therapy

Background and Purpose: Candida parapsilosis isolates usually have a low minimum inhibitory concentration (MIC) against azoles. Although Candida parapsilosis isolates usually have low MICs against azoles, recent studies candida invasive infections due to azole resistant-C. parapsilosis isolates . Regarding this, the main aim of this study was to determine the susceptibility pattern of Iranian clinical C. parapsilosis against available azole antifungal drugs. Materials and Methods: This study was conducted on 105 previously-identified isolates of C. parapsilosis sensu stricto. For the purpose of the study, the isolates were subjected to antifungal susceptibility testing against fluconazole (FLZ), itraconazole (ITZ), voriconazole (VRZ), and two new azole drugs, namely luliconazole (LUZU) and lanoconazole (LZN). The broth microdilution reference method adopted in this study was according to the Clinical & Laboratory Standards Institute M27-A3 and M27-S4 documents. Results: According to the results, 89% (n=94) of C. parapsilosis isolates showed a MIC of ≥ 1 µg/ml, indicating resistance against ITZ. Multi-azole resistance was observed in 3.8% of the isolates. In addition, LUZU and LZN demonstrated the highest efficacy with the MIC50 values of 0.5 and 1 µg/ml, respectively. Conclusion: The majority of the isolates showed high MIC values against ITZ. This may have been associated with the long-term ITZ prophylaxis/therapy in patients infected with candidiasis. Hence, the adoption of an appropriate antifungal agent is a crucial step for starting the treatment.

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