scholarly journals New species within Candida parapsilosis and Candida glabrata

2019 ◽  
pp. 51-57
Author(s):  
Dariusz Domański ◽  
Magdalena Anna Sikora ◽  
Robert Tomasz Kuthan ◽  
Ewa Augustynowicz-Kopeć ◽  
Ewa Swoboda-Kopeć
Keyword(s):  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Secondary Alcohol ◽  
Sensu Stricto ◽  
Diagnostic Methods ◽  
D2 Domain ◽  
Dehydrogenase Gene ◽  
Pcr Rflp ◽  
Secondary Alcohol Dehydrogenase ◽  
Candida Nivariensis

Introduction: Candida parapsilosis and Candida glabrata are another yeasts that form complexes of crypospecies. Although these species have been described more than a decade ago, knowledge about them is still limited. The reason for this is the large phenotypic similarity that unables them from being differentiated by classical diagnostic methods. The aim of the study was to identify species of clinical strains within C. glabrata and C. parapsilosis complexes. Material and methods: Standard PCR-RFLP of the secondary alcohol dehydrogenase gene (SADH) with BanI restriction enzyme served to determine species affiliation within the C. parapsilosis complex. The internal transcribed spacer was used to confirm the identification of C. glabrata sensu stricto. The D1/D2 domain of the 26S rDNA gene was sequenced in order to identify C. nivariensis and C. bracarensis strains. Results: As a result of the molecular analysis, 24 Candida nivariensis isolates and 4 C. metapsilosis strains and 9 C. orthopsilosis strains were detected. Conclusions: Prevalence of new cryptic species was relatively low.

scholarly journals Genome Editing of a Secondary Alcohol Dehydrogenase Gene by CRISPR‐Cas9 Technology

2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Jenq‐Kuen Huang ◽  
Kadidia Samassekou ◽  
Shawn McClenahan ◽  
Jacob Seiver ◽  
Scott Holt ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Genome Editing ◽  
Secondary Alcohol ◽  
Dehydrogenase Gene ◽  
Secondary Alcohol Dehydrogenase

scholarly journals Candidemia and its Risk Factors in Neonates and Children

2020 ◽  
Vol 8 (4) ◽  
Author(s):  
Azam Fattahi ◽  
Ensieh Lotfali ◽  
Hossein Masoumi-Asl ◽  
Shirin Sayyahfar ◽  
Majid Kalani ◽  
...  
Keyword(s):  
Risk Factors ◽  
Candida Albicans ◽  
Blood Culture ◽  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Species Level ◽  
Candida Spp ◽  
Pcr Rflp ◽  
Candida Parapsilosis Complex ◽  
Isolated Species

Objectives: The present study was conducted to raise attention to the frequency of Candida spp. and evaluation of risk factors of candidemia in hospitalized neonates and children. Methods: Identification of Candida at species level was done using the PCR-RFLP method. The Candida albicans complex and Candida parapsilosis complex were differentiated using the HWP1 gene amplification and PCR-RFLP with NlaIII restriction enzyme, respectively. Results: Out of 75 blood culture specimens, 42 (84%) cases were positive for Candida spp. of whom 30 (71.42%) and 12 (28.57%) cases were female and male, respectively. Thirty-two (76%) candidemia were presented in pediatrics with 6 years up to 12 years, 10 (23.80%) in neonates of one month or less. In the present study, Candida parapsilosis (n =25; 59.52%) was the most prevalent isolated species followed by C. albicans (n =11; 26.19%), C. tropicalis (n =4; 9.52%), and Candida glabrata (n =2; 4.76%). Conclusions: According to potentially dangerous complications of bloodstream infection by Candida spp. in neonates and children, it is necessary to identify and eliminate the underlying conditions and risk factors of this disease.

scholarly journals Molecular screening for Candida orthopsilosis and Candidametapsilosis among Danish Candida parapsilosis group blood cultureisolates: proposal of a new RFLP profile for differentiation

2010 ◽  
Vol 59 (4) ◽  
pp. 414-420 ◽  
Author(s):  
Hossein Mirhendi ◽  
Brita Bruun ◽  
Henrik Carl Schønheyder ◽  
Jens Jørgen Christensen ◽  
Kurt Fuursted ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Profile Analysis ◽  
Secondary Alcohol ◽  
Pcr Amplification ◽  
Rrna Gene ◽  
Rflp Profile ◽  
Pcr Rflp ◽  
Invasive Infections ◽  
Candida Orthopsilosis ◽  
26S Rrna

Candida orthopsilosis and Candida metapsilosis are recentlydescribed species phenotypically indistinguishable from Candida parapsilosis. We evaluated phenotyping and molecular methods for the detection ofthese species among 79 unique blood culture isolates of the C. parapsilosis group obtained during the years 2004–2008. The isolates were screenedby PCR amplification of the secondary alcohol dehydrogenase-encoding gene (SADH) followed by digestion with the restriction enzyme BanI, using C. parapsilosis ATCC 22019, C. orthopsilosisATCC 96139 and C. metapsilosis ATCC 96144 as controls. Isolates withRFLP patterns distinct from C. parapsilosis were characterized bysequence analysis of the ITS1–ITS2, 26S rRNA (D1/D2) and SADH regions. Restriction patterns for the 3 species with each of 610restriction enzymes were predicted in silico using 12 available sequences.By PCR-RFLP of the SADH gene alone, four isolates (5.1 %)had a pattern identical to the C. orthopsilosis reference strain.Sequence analysis of SADH and ITS (internal transcribed spacer)regions identified two of these isolates as C. metapsilosis. Theseresults were confirmed by creating a phylogenetic tree based on concatenatedsequences of SADH, ITS and 26S rRNA gene sequence regions. Optimaldifferentiation between C. parapsilosis, C. metapsilosisand C. orthopsilosis was predicted using digestion with NlaIII,producing discriminatory band sizes of: 131 and 505 bp; 74, 288 and 348 bp;and 131, 217 and 288 bp, respectively. This was confirmed using the referencestrains and 79 clinical isolates. In conclusion, reliable discrimination wasobtained by PCR-RFLP profile analysis of the SADH gene after digestionwith NlaIII but not with BanI. C. metapsilosisand C. orthopsilosis are involved in a small but significant numberof invasive infections in Denmark.

scholarly journals A case series of medically managed Candida parapsilosis complex prosthetic valve endocarditis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Penghao Guo ◽  
Yuting He ◽  
Rui Fan ◽  
Zhongwen Wu ◽  
Yili Chen ◽  
...  
Keyword(s):  
Prosthetic Valve ◽  
Candida Parapsilosis ◽  
Case Series ◽  
Prosthetic Valve Endocarditis ◽  
Sensu Stricto ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Tof Ms

Abstract Background In recent years, Candida parapsilosis is recognized as a species complex and is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis. Candida parapsilosis complex prosthetic valve endocarditis (PVE) is rare and the survival rate is still low despite of optimal therapeutic strategies. In our report, it is novel to report cases as Candida parapsilosis complex PVE at species and identify Candida parapsilosis using MALDI-TOF MS. Case presentation A series of 4 cases of Candida parapsilosis complex PVE from our institution was reported. Three were infected by Candida parapsilosis sensu stricto and one was infected by Candida metapsilosis. The condition of two cases got better and the other died. Conclusions More attention should be paid to Candida parapsilosis complex PVE and early diagnosis and prompt antibiotic therapy may play a role in the treatment for Candida parapsilosis complex PVE. It is recommended to identify Candida parapsilosis complex at species level and MALDI-TOF MS as an easy, fast and efficient identification method is worth promoting in clinical microbiology

scholarly journals High Frequency of Cryptosporidium hominis Infecting Infants Points to A Potential Anthroponotic Transmission in Maputo, Mozambique

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 293
Author(s):  
Idalécia Cossa-Moiane ◽  
Hermínio Cossa ◽  
Adilson Fernando Loforte Bauhofer ◽  
Jorfélia Chilaúle ◽  
Esperança Lourenço Guimarães ◽  
...  
Keyword(s):  
Public Hospitals ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
P Value ◽  
Cryptosporidium Hominis ◽  
Cryptosporidium Spp ◽  
Pcr Rflp ◽  
Maputo City ◽  
Stool Samples ◽  
Polymerase Chain

Cryptosporidium is one of the most important causes of diarrhea in children less than 2 years of age. In this study, we report the frequency, risk factors and species of Cryptosporidium detected by molecular diagnostic methods in children admitted to two public hospitals in Maputo City, Mozambique. We studied 319 patients under the age of five years who were admitted due to diarrhea between April 2015 and February 2016. Single stool samples were examined for the presence of Cryptosporidium spp. oocysts, microscopically by using a Modified Ziehl–Neelsen (mZN) staining method and by using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) technique using 18S ribosomal RNA gene as a target. Overall, 57.7% (184/319) were males, the median age (Interquartile range, IQR) was 11.0 (7–15) months. Cryptosporidium spp. oocysts were detected in 11.0% (35/319) by microscopy and in 35.4% (68/192) using PCR-RFLP. The most affected age group were children older than two years, [adjusted odds ratio (aOR): 5.861; 95% confidence interval (CI): 1.532–22.417; p-value < 0.05]. Children with illiterate caregivers had higher risk of infection (aOR: 1.688; 95% CI: 1.001–2.845; p-value < 0.05). An anthroponotic species C. hominis was found in 93.0% (27/29) of samples. Our findings demonstrated that cryptosporidiosis in children with diarrhea might be caused by anthroponomic transmission.

scholarly journals Genome and transcriptome of a pathogenic yeast, Candida nivariensis

2021 ◽  
Author(s):  
Yunfan Fan ◽  
Andrew N Gale ◽  
Anna Bailey ◽  
Kali Barnes ◽  
Kiersten Colotti ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Glabrata ◽  
Nanopore Sequencing ◽  
Cdna Sequencing ◽  
Pathogenic Yeast ◽  
Mitochondrial Sequence ◽  
Dna And Rna ◽  
Oxford Nanopore ◽  
Candida Nivariensis ◽  
Oxford Nanopore Technologies

Abstract We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies (ONT) and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.

scholarly journals Antifungal Activity of SCY-078 and Standard Antifungal Agents against 178 Clinical Isolates of Resistant and Susceptible Candida Species

2017 ◽  
Vol 61 (11) ◽  
Author(s):  
Wiley A. Schell ◽  
A. M. Jones ◽  
Katyna Borroto-Esoda ◽  
Barbara D. Alexander
Keyword(s):  
Candida Glabrata ◽  
Antifungal Agents ◽  
Candida Parapsilosis ◽  
Candida Krusei ◽  
Wild Type ◽  
Content Type ◽  
Clinical Resistance ◽  
Excellent Activity ◽  
Candida Lusitaniae

ABSTRACT SCY-078 in vitro activity was determined for 178 isolates of resistant or susceptible Candida albicans, Candida dubliniensis, Candida glabrata, Candida krusei, Candida lusitaniae, and Candida parapsilosis, including 44 Candida isolates with known genotypic (FKS1 or FKS2 mutations), phenotypic, or clinical resistance to echinocandins. Results were compared to those for anidulafungin, caspofungin, micafungin, fluconazole, and voriconazole. SCY-078 was shown to have excellent activity against both wild-type isolates and echinocandin- and azole-resistant isolates of Candida species.

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