Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering

Three-dimensional (3D) highly porous poly (DL-lactic-co-glycolic acid)/tricalcium phosphate (PLGA/TCP) scaffolds were fabricated using a rapid prototyping technique (RP). The biopolymer carriers (4mm×4mm×4mm) subsequently were coated with collagen type I (Col) to produce PLGA/TCP/Col composites and utilized as an extracellular matrix for a cell-based strategy of bone tissue engineering. Autologous bone marrow stromal cells (BMSCs) harvested from New Zealand white rabbits were cultured under an osteogenic condition (BMSCs-OB) followed by seeding into the structural highly porous PLGA/TCP/Col composites (i.e. PLGA/TCP/Col/BMSCs-OB). Scanning electron microscopy observation found that the RP-based scaffolds had appropriate microstructure, controlled interconnectivity and high porosity. Modification of the scaffolds with collagen type I (PLGA/TCP/Col) essentially increased the affinity of the carriers to seeding cells, and PLGA/TCP/Col composites were well biocompatible with BMSCs-OB. The PLGA/TCP/Col/BMSCs-OB constructs were then subcutaneously implanted in the back of rabbits compared to controls with autologous BMSCs suspension and carriers alone. As a result, histological new bone formation was observed only in the experimental group with PLGA/TCP/Col/BMSCs-OB constructs 8 weeks after implantation. In the control group with scaffold alone only biodegradation of the carriers was found. Therefore, these results validate our bio-manufacturing methods for a new bone graft substitute.

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Ultrarapid Purification of Collagen Type I for Tissue Engineering Applications

Tissue Engineering Part C Methods ◽

10.1089/ten.tec.2010.0720 ◽

2011 ◽

Vol 17 (9) ◽

pp. 879-885 ◽

Cited By ~ 12

Author(s):

Christina A. Pacak ◽

Jared M. Powers ◽

Douglas B. Cowan

Keyword(s):

Tissue Engineering ◽

Collagen Type ◽

Collagen Type I ◽

Type I ◽

Engineering Applications

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Characterization of Collagen Type I and II Blended Hydrogels for Articular Cartilage Tissue Engineering

Biomacromolecules ◽

10.1021/acs.biomac.6b00684 ◽

2016 ◽

Vol 17 (10) ◽

pp. 3145-3152 ◽

Cited By ~ 20

Author(s):

Nelda Vázquez-Portalatı́n ◽

Claire E. Kilmer ◽

Alyssa Panitch ◽

Julie C. Liu

Keyword(s):

Tissue Engineering ◽

Articular Cartilage ◽

Collagen Type ◽

Cartilage Tissue Engineering ◽

Collagen Type I ◽

Cartilage Tissue ◽

Type I

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PLGA-collagen-BPNS Bifunctional Composite Mesh for Photothermal Therapy of Melanoma and Skin Tissue Engineering

Journal of Materials Chemistry B ◽

10.1039/d1tb02366g ◽

2021 ◽

Author(s):

Linawati Sutrisno ◽

Huajian Chen ◽

Toru Yoshitomi ◽

Naoki Kawazoe ◽

Yingnan Yang ◽

...

Keyword(s):

Tissue Engineering ◽

Skin Cancer ◽

Cancer Cells ◽

Photothermal Therapy ◽

Glycolic Acid ◽

Composite Scaffold ◽

Skin Regeneration ◽

Skin Tissue ◽

Composite Mesh ◽

Skin Tissue Engineering

Treatment of melanoma requires not only the elimination of skin cancer cells but also skin regeneration to heal defects. To achieve this goal, bifunctional composite scaffold of poly(DL-lactic-co-glycolic acid) (PLGA),...

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Labeling of Collagen Type I Templates with a Naturally Derived Contrast Agent for Noninvasive MR Imaging in Soft Tissue Engineering

Advanced Healthcare Materials ◽

10.1002/adhm.201800605 ◽

2018 ◽

Vol 7 (18) ◽

pp. 1800605 ◽

Cited By ~ 3

Author(s):

Heinz P. Janke ◽

Nihan Güvener ◽

Weiqiang Dou ◽

Dorien M. Tiemessen ◽

Anglita YantiSetiasti ◽

...

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Mr Imaging ◽

Contrast Agent ◽

Collagen Type ◽

Collagen Type I ◽

Type I ◽

Soft Tissue Engineering

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Fullerene-C60/liposome complex: Defensive effects against UVA-induced damages in skin structure, nucleus and collagen type I/IV fibrils, and the permeability into human skin tissue

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2009.11.010 ◽

2010 ◽

Vol 98 (1) ◽

pp. 99-105 ◽

Cited By ~ 22

Author(s):

Shinya Kato ◽

Hisae Aoshima ◽

Yasukazu Saitoh ◽

Nobuhiko Miwa

Keyword(s):

Human Skin ◽

Collagen Type ◽

Collagen Type I ◽

Fullerene C60 ◽

Skin Tissue ◽

Type I ◽

Skin Structure ◽

Human Skin Tissue

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Collagen Type I Biomaterials as Scaffolds for Bone Tissue Engineering

Polymers ◽

10.3390/polym13040599 ◽

2021 ◽

Vol 13 (4) ◽

pp. 599

Author(s):

Gustavo A. Rico-Llanos ◽

Sara Borrego-González ◽

Miguelangel Moncayo-Donoso ◽

José Becerra ◽

Rick Visser

Keyword(s):

Tissue Engineering ◽

Extracellular Matrix ◽

Mechanical Strength ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Collagen Type ◽

Collagen Type I ◽

Current Status ◽

Type I ◽

Organic Constituent

Collagen type I is the main organic constituent of the bone extracellular matrix and has been used for decades as scaffolding material in bone tissue engineering approaches when autografts are not feasible. Polymeric collagen can be easily isolated from various animal sources and can be processed in a great number of ways to manufacture biomaterials in the form of sponges, particles, or hydrogels, among others, for different applications. Despite its great biocompatibility and osteoconductivity, collagen type I also has some drawbacks, such as its high biodegradability, low mechanical strength, and lack of osteoinductive activity. Therefore, many attempts have been made to improve the collagen type I-based implants for bone tissue engineering. This review aims to summarize the current status of collagen type I as a biomaterial for bone tissue engineering, as well as to highlight some of the main efforts that have been made recently towards designing and producing collagen implants to improve bone regeneration.

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Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits

Stem Cell Research & Therapy ◽

10.1186/s13287-021-02638-6 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Jiyuan Yan ◽

Chaoxu Liu ◽

Chang Tu ◽

Ruizhuo Zhang ◽

Xiangyu Tang ◽

...

Keyword(s):

Chondrogenic Differentiation ◽

Collagen Type ◽

Composite Scaffold ◽

Low Frequency ◽

Collagen Type I ◽

Type I ◽

Osteochondral Defects ◽

Osteochondral Repair

Abstract Background Cartilage damage is a common medical issue in clinical practice. Complete cartilage repair remains a significant challenge owing to the inferior quality of regenerative tissue. Safe and non-invasive magnetic therapy combined with tissue engineering to repair cartilage may be a promising breakthrough. Methods In this study, a composite scaffold made of Hydroxyapatite-Collagen type-I (HAC) and PLGA-PEG-PLGA thermogel was produced to match the cartilage and subchondral layers in osteochondral defects, respectively. Bone marrow mesenchymal stem cells (BMSC) encapsulated in the thermogel were stimulated by an electromagnetic field (EMF). Effect of EMF on the proliferation and chondrogenic differentiation potential was evaluated in vitro. 4 mm femoral condyle defect was constructed in rabbits. The scaffolds loaded with BMSCs were implanted into the defects with or without EMF treatment. Effects of the combination treatment of the EMF and composite scaffold on rabbit osteochondral defect was detected in vivo. Results In vitro experiments showed that EMF could promote proliferation and chondrogenic differentiation of BMSCs partly by activating the PI3K/AKT/mTOR and Wnt1/LRP6/β-catenin signaling pathway. In vivo results further confirmed that the scaffold with EMF enhances the repair of osteochondral defects in rabbits, and, in particular, cartilage repair. Conclusion Hydrogel-Hydroxyapatite-Monomeric Collagen type-I scaffold with low-frequency EMF treatment has the potential to enhance osteochondral repair.

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