Ultrarapid Purification of Collagen Type I for Tissue Engineering Applications

Three-dimensional (3D) highly porous poly (DL-lactic-co-glycolic acid)/tricalcium phosphate (PLGA/TCP) scaffolds were fabricated using a rapid prototyping technique (RP). The biopolymer carriers (4mm×4mm×4mm) subsequently were coated with collagen type I (Col) to produce PLGA/TCP/Col composites and utilized as an extracellular matrix for a cell-based strategy of bone tissue engineering. Autologous bone marrow stromal cells (BMSCs) harvested from New Zealand white rabbits were cultured under an osteogenic condition (BMSCs-OB) followed by seeding into the structural highly porous PLGA/TCP/Col composites (i.e. PLGA/TCP/Col/BMSCs-OB). Scanning electron microscopy observation found that the RP-based scaffolds had appropriate microstructure, controlled interconnectivity and high porosity. Modification of the scaffolds with collagen type I (PLGA/TCP/Col) essentially increased the affinity of the carriers to seeding cells, and PLGA/TCP/Col composites were well biocompatible with BMSCs-OB. The PLGA/TCP/Col/BMSCs-OB constructs were then subcutaneously implanted in the back of rabbits compared to controls with autologous BMSCs suspension and carriers alone. As a result, histological new bone formation was observed only in the experimental group with PLGA/TCP/Col/BMSCs-OB constructs 8 weeks after implantation. In the control group with scaffold alone only biodegradation of the carriers was found. Therefore, these results validate our bio-manufacturing methods for a new bone graft substitute.

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Characterization of Collagen Type I and II Blended Hydrogels for Articular Cartilage Tissue Engineering

Biomacromolecules ◽

10.1021/acs.biomac.6b00684 ◽

2016 ◽

Vol 17 (10) ◽

pp. 3145-3152 ◽

Cited By ~ 20

Author(s):

Nelda Vázquez-Portalatı́n ◽

Claire E. Kilmer ◽

Alyssa Panitch ◽

Julie C. Liu

Keyword(s):

Tissue Engineering ◽

Articular Cartilage ◽

Collagen Type ◽

Cartilage Tissue Engineering ◽

Collagen Type I ◽

Cartilage Tissue ◽

Type I

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Labeling of Collagen Type I Templates with a Naturally Derived Contrast Agent for Noninvasive MR Imaging in Soft Tissue Engineering

Advanced Healthcare Materials ◽

10.1002/adhm.201800605 ◽

2018 ◽

Vol 7 (18) ◽

pp. 1800605 ◽

Cited By ~ 3

Author(s):

Heinz P. Janke ◽

Nihan Güvener ◽

Weiqiang Dou ◽

Dorien M. Tiemessen ◽

Anglita YantiSetiasti ◽

...

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Mr Imaging ◽

Contrast Agent ◽

Collagen Type ◽

Collagen Type I ◽

Type I ◽

Soft Tissue Engineering

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Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering

Materials Science and Engineering C ◽

10.1016/j.msec.2013.09.043 ◽

2014 ◽

Vol 34 ◽

pp. 402-409 ◽

Cited By ~ 120

Author(s):

Sneh Gautam ◽

Chia-Fu Chou ◽

Amit K. Dinda ◽

Pravin D. Potdar ◽

Narayan C. Mishra

Keyword(s):

Tissue Engineering ◽

Surface Modification ◽

Collagen Type ◽

Composite Scaffold ◽

Collagen Type I ◽

Skin Tissue ◽

Type I ◽

Skin Tissue Engineering

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Collagen Type I Biomaterials as Scaffolds for Bone Tissue Engineering

Polymers ◽

10.3390/polym13040599 ◽

2021 ◽

Vol 13 (4) ◽

pp. 599

Author(s):

Gustavo A. Rico-Llanos ◽

Sara Borrego-González ◽

Miguelangel Moncayo-Donoso ◽

José Becerra ◽

Rick Visser

Keyword(s):

Tissue Engineering ◽

Extracellular Matrix ◽

Mechanical Strength ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Collagen Type ◽

Collagen Type I ◽

Current Status ◽

Type I ◽

Organic Constituent

Collagen type I is the main organic constituent of the bone extracellular matrix and has been used for decades as scaffolding material in bone tissue engineering approaches when autografts are not feasible. Polymeric collagen can be easily isolated from various animal sources and can be processed in a great number of ways to manufacture biomaterials in the form of sponges, particles, or hydrogels, among others, for different applications. Despite its great biocompatibility and osteoconductivity, collagen type I also has some drawbacks, such as its high biodegradability, low mechanical strength, and lack of osteoinductive activity. Therefore, many attempts have been made to improve the collagen type I-based implants for bone tissue engineering. This review aims to summarize the current status of collagen type I as a biomaterial for bone tissue engineering, as well as to highlight some of the main efforts that have been made recently towards designing and producing collagen implants to improve bone regeneration.

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In Vitro Osteogenic, Angiogenic, and Inflammatory Effects of Copper in β-Tricalcium Phosphate

MRS Advances ◽

10.1557/adv.2018.686 ◽

2019 ◽

Vol 4 (21) ◽

pp. 1253-1259

Author(s):

Weiguo Han ◽

Haley Cummings ◽

Murali Krishna Duvuuru ◽

Sarah Fleck ◽

Sahar Vahabzadeh ◽

...

Keyword(s):

Tissue Engineering ◽

Tricalcium Phosphate ◽

Physical And Mechanical Properties ◽

Early Time ◽

Collagen Type I ◽

Type I ◽

Dependent Manner ◽

Dental Tissue ◽

Engineering Applications

AbstractTricalcium phosphate (TCP) is a promising candidate in bone and dental tissue engineering applications. Though osteoconductive, its low osteoinductivity is a major concern. Trace elements addition at low concentrations are known for their impact on not only the osteoinductivity, but also physical and mechanical properties of TCP. Copper (Cu) is known for its role in vascularization and angiogenesis in biological systems. Here, we studied the effects of Cu addition on phase composition, porosity, microstructure and in vitro interaction with osteoblast (OB) cells. Our results showed that Cu stabilized the TCP structure, while no significant effect of microstructure and porosity was found. Cu at concentrations less than 1 wt.% did not have any cytotoxic effect while decreased proliferation of OBs were observed at 1 wt.% Cu doped TCP. Addition of Cu upregulated collagen type I and vascular endothelial growth factor expression in a dose dependent manner at early time-point. Furthermore, Cu reduced inflammatory gene expression by human osteoblasts. These findings show that addition of Cu to TCP may provide a therapeutic strategy that can be applied in bone tissue engineering applications.

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A 3D-Printed PLCL Scaffold Coated with Collagen Type I and Its Biocompatibility

BioMed Research International ◽

10.1155/2018/5147156 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Yong He ◽

Wei Liu ◽

Lianxiong Guan ◽

Jielin Chen ◽

Li Duan ◽

...

Keyword(s):

Tissue Engineering ◽

Collagen Type ◽

Articular Chondrocytes ◽

Mechanical Test ◽

Great Influence ◽

Collagen Type I ◽

Blue Staining ◽

Type I ◽

3D Printed ◽

Better Than

Scaffolds play an important role in tissue engineering and their structure and biocompatibility have great influence on cell behaviors. In this study, poly(l-lactide-co-ε-caprolactone) (PLCL) scaffolds were printed by a 3D printing technology, low-temperature deposition manufacturing (LDM), and then PLCL scaffolds were treated by alkali and coated with collagen type I (COLI). The scaffolds were characterized by scanning electron microscopy (SEM), porosity test, mechanical test, and infrared spectroscopy. The prepared PLCL and PLCL-COLI scaffolds had three-dimensional (3D) porous structure and they not only have macropores but also have micropores in the deposited lines. Although the mechanical property of PLCL-COLI was slightly lower than that of PLCL scaffold, the hydrophilicity of PLCL-COLI was significantly enhanced. Rabbit articular chondrocytes were extracted and were identified as chondrocytes by toluidine blue staining. To study the biocompatibility, the chondrocytes were seeded on scaffolds for 1, 3, 5, 7, and 10 days. MTT assay showed that the proliferation of chondrocytes on PLCL-COLI scaffold was better than that on PLCL scaffold. And the morphology of cells on PLCL-COLI after 1-day culture was much better than that on PLCL. This 3D-printed PLCL scaffold coated with COLI shows a great potential application in tissue engineering.

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